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antisense orfs, codon bias and the evo lu tion of the ge netic code
antisense orfs, codon bias and the evo lu tion of the ge netic code

... These and other data suggest that the SCOR families of enzymes diverged from a common ancestor that evolved before the AT-rich half of the genetic code was defined. This work is supported in part by NIH Grant No DK26546. ...
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No Slide Title

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Life and Chemistry: Large molecules: Proteins

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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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