• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
SECTION 2 - CELL FUNCTION AND BIOCHEMICAL MEASUREMENT
SECTION 2 - CELL FUNCTION AND BIOCHEMICAL MEASUREMENT

... 11. Plasma proteins contribute to the colloid osmotic pressure of the blood, which is needed for fluid balance. Plasma proteins also serve as circulating enzymes, hormones, transport molecules, and antibodies. Although the liver is a major source of plasma proteins, these molecules can originate fro ...
Functional analysis of a type-2C protein phosphatase (AtPP2C52) in
Functional analysis of a type-2C protein phosphatase (AtPP2C52) in

... Therefore, the activities of PP2Cs may controlled predominantly by their tissue- or cell type-specific expression, subcellular compartmentalization,post-translational modification, or/and degradation (Lammers and Lavi, 2007). In Arabidopsis, seventy-six PP2C genes were identified (Kerk et al., 2002) ...
Proteins
Proteins

Improving protein fold recognition with hybrid
Improving protein fold recognition with hybrid

... consequently, comparative modeling (Müller et al., 1999; Park et al., 1998). Fold recognition was later taken to a new level with the FFAS method (Rychlewski et al., 2000), which was based on profile–profile alignment. By using profiles for both target and template, such methods fully exploit the t ...
Document
Document

... Annotations ...
What makes cell membranes work? 9/23
What makes cell membranes work? 9/23

... Raise Tm ...
The effect of pH on the digestion of proteins in vitro by pepsin
The effect of pH on the digestion of proteins in vitro by pepsin

... peptides from any of the digests of the soya-protein samples by either electrophoretic or chromatographic methods. DISCUSSION ...
The Cytoskeleton
The Cytoskeleton

... - Regulation of subunit synthesis and degradation - Regulation of nucleation - Regulation by changing environmental conditions - Regulation by modifying subunits - Regulation using accessory proteins ...
lecture 3
lecture 3

... These molecules are called amphiphilic because they are composed of two parts which differ by their solubility in water: (1) polar «head» possessing high affinity for water, i.e. hydrophilic, and (2) .tail» that is formed by non-polar carbohydrate chains of fatty acids; this part of the molecule has ...
Document
Document

... • DNA contains genes, sequences of nucleotide bases • These Genes code for polypeptides (proteins) • Proteins are used to build cells and do much of the work inside cells copyright cmassengale ...
Structure-function of the ADP/ATP carrier
Structure-function of the ADP/ATP carrier

... [ 1 1. Thus. any information gained about the structure-function relationships of the (Ca’+-Mg”)ATPase mill provide important insights into the working of other members of this family of transporters. I he series of events which lead t o cation translocation has been elucidated in some detail for a ...
“Beneficial” Metals and Microbes: Interactions involving
“Beneficial” Metals and Microbes: Interactions involving

... catalysis, redox centers, structure. ...
Characterisation of the Aspergillus niger dapB gene, which encodes
Characterisation of the Aspergillus niger dapB gene, which encodes

... genomic DNA of A. niger. The 144-bp fragment was then used as a probe to screen a genomic library of A. niger constructed in bacteriophage k. Two positive clones were identified and subsequently purified. Southern analysis of the DNAs from these phages with the 144-bp fragment resulted in the identific ...
Macromolecules: Their Structure and Function A. Lipids: Water
Macromolecules: Their Structure and Function A. Lipids: Water

... of Amino Acids • The tertiary structure is generated by bending and folding of the polypeptide chain. • The quaternary structure is the arrangement of polypeptides in a single functional unit consisting of more than one polypeptide subunit. Figure 3.5 – Part 2 ...
myosinActivity.pdf
myosinActivity.pdf

... occasionally use genotype information in deciding how to treat people with this disorder. People who have serious symptoms and active disease are usually treated for symptom relief regardless of genotype. However, in some cases, individuals remain asymptomatic until sudden heart failure during exerc ...
Perspectives
Perspectives

... dimer. Each ␣-chain active site is connected to a ␤-chain active site by an intraenzyme tunnel, and indole, enzymatically generated from indoleglycerol phosphate in the ␣-active site, traverses through this tunnel to the ␤-active site, where it is combined with l-serine to form l-tryptophan. In the ...
Max1
Max1

... protein where a ligand could potentially bind  Funnel of Attraction – all ligand conformations within 10 Å RMSD of a binding site conformation ...
GENE NOMENCLATURE OF DIFFERENT TYPES OF SPINOCEREBELLAR ATAXIA (SCA) AND
GENE NOMENCLATURE OF DIFFERENT TYPES OF SPINOCEREBELLAR ATAXIA (SCA) AND

... distinct genetic causes of SCA are known and each of which could be considered a disease in its own right. The nomenclature of 36 types of Spinocerebellar ataxia and a computational work on Spinocerebellar ataxia type 1 (SCA1) are done in this study. SCA1 is a progressive, degenerative and often fat ...
Early Cleavage Media
Early Cleavage Media

... ASSISTED REPRODUCTIVE TECHNOLOGY ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... Hydrolysis: Chemical reaction that uses water to separate polymers into monomers. (Break apart) - Exactly the opposite of Dehydration synthesis ...
wt - EMBO
wt - EMBO

... Rotating disc has thousands of pinholes field of view is scanned in single exposure, thus allowing the capture of confocal images at high speed (great for z-stacks) multiple points are collected simultaneously, sample receives less laser light spinning disk confocal microscopy is well suited to high ...
Autism
Autism

... exchange with the zinc in the blood plasma. Zinc is also part of the make-up of over 300 enzymes and hundreds of zinc binding proteins. It is used in many of the body’s systemic protein interactions as well as in the synthesis of the hormone melatonin and its precursor, seratonin, and it facilitates ...
Protein
Protein

... Other foods consumed at the same time can change the digestibility ...
Chapter 3 The Molecules of Cells
Chapter 3 The Molecules of Cells

... can regulate enzyme activity in a cell ▪ Enzyme inhibitors are important in regulating cell metabolism – Often the product of a metabolic pathway can serve as an inhibitor of one enzyme in the pathway, a mechanism called feedback inhibition – The more product formed, the greater the inhibition, and ...
Honors Biology Unit 1 Objectives: The Chemistry of Life
Honors Biology Unit 1 Objectives: The Chemistry of Life

... triglyceride, protein, amino acid, polypeptide, peptide bond, primary through quaternary protein structure, polar, nonpolar, nucleic acid, nucleotide, RNA, DNA, gene, double helix. 2. Explain the relationship(s) among atoms, molecules, elements, and compounds. 3. Describe the three types of chemical ...
< 1 ... 209 210 211 212 213 214 215 216 217 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report