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PowerPoint Format - Kinexus Bioinformatics Corporation
PowerPoint Format - Kinexus Bioinformatics Corporation

...  Identifies differentially expressed genes in 50 types of human cancers  Identifies genes that are highly co-expressed  Shows tissue and cell relatedness ...
1 Irreversible Enzyme Inhibition Affinity labels for studying enzyme
1 Irreversible Enzyme Inhibition Affinity labels for studying enzyme

... • DFP reacts with the active site serine (Ser-195) of chymotrypsin to form DFP-chymotrypsin • Such organophosphorous inhibitors are used as insecticides or for enzyme research • These inhibitors are toxic because they inhibit acetylcholinesterase (a serine protease that hydrolyzes the neurotransmitt ...
Alignment
Alignment

... • PAM was developed by Margaret Dayhoff and co-workers in 1978 by examining 1572 mutations between 71 families of closely related proteins • BLOSUM is more recent and computed from blocks of sequences with sufficient ...
Viral Antigens Recombinant Proteins
Viral Antigens Recombinant Proteins

... Meridian Life Science, (MLS) is an industry leader in the commercial production of critical raw materials for the diagnostic market. MLS has a proven track record gained from over 25 years of cell culture, viral propagation, and protein purification experience. MLS has the expertise to develop and p ...
Biomolecules
Biomolecules

... classified as α, β, γ, δ and so on. Only α-amino acids are obtained on hydrolysis of proteins. They NH2 may contain other functional groups also. a-amino acid All α-amino acids have trivial names, which (R = side chain) usually reflect the property of that compound or its source. Glycine is so named ...
Chapter 12 Pathways to biomolecules
Chapter 12 Pathways to biomolecules

Slide 1
Slide 1

The significance of biochemical and molecular sample integrity in
The significance of biochemical and molecular sample integrity in

Bovine Peptidoglycan Recognition Protein
Bovine Peptidoglycan Recognition Protein

... bovine PGRP-S under conditions approximating the extracellular milieu by modulating cations, ionic strength, osmolality, and serum concentration. The complete set of molecular targets for PGRPs has yet to be elucidated. Our previous results provided evidence that bPGRP-S could act in a PGN-independe ...
Planta
Planta

... proteins to the C terminus of VvCTr1, the stop codon was omitted in the reverse primer. For subcellular localization studies, the entry clone carrying VvCTr1 was recombined into pH7FWG2 for green fluorescent protein (GFP) fusion and pH7RWG2 for red fluorescent protein (RFP) fusion (Karimi et al. 200 ...
Protein synthesis (Primer)
Protein synthesis (Primer)

... Central Dogma of Molecular Biology states that information flow from DNA Î RNA Î protein DNA is made of phosphonucleotides (phosphoate + sugar + base) Genetic information is encoded in a string of DNA bases (A,C,G,T) Hydrogen bond mediated base pairing (A**T, C**G) protects DNA bases from chemical d ...
Expt. 2 Bioinformatics
Expt. 2 Bioinformatics

TD3 5.08 – PKS/NRPS pathways and mass
TD3 5.08 – PKS/NRPS pathways and mass

... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c
EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c

... Phe is 4.7 nM. 8% of the total sample was used for GC analysis or approximately 0.4 nM of Phe. This amount of material is close to the confidence limits of the assay. Thus, any other amino acids present at 50% or less of the major residue will likely be undetected. Heterogeneity in the early portion ...
urea cycle
urea cycle

... • Describe digestion of proteins, absorption of amino acids in intestine and transport of through blood • Describe some compounds made from amino acids • Describe role of intracellular proteases, proteasome in recycling proteins • Explain the essentials of the urea cycle for elimination of nitrogen ...
View Full PDF - Biochemical Society Transactions
View Full PDF - Biochemical Society Transactions

... produce a secondary metabolite, now known as rapamycin, with potent antifungal activity [1,2]. Not long after its initial characterization as an antifungal agent, rapamycin was found to possess cytostatic activity not only against lower eukaryotes but also against mammalian cells, particularly immun ...
Intermediary metabolism
Intermediary metabolism

... (irreverzible)  low concentration within a cell ...
Immunoinformatics Study of gp120 of Human Immunodeficiency
Immunoinformatics Study of gp120 of Human Immunodeficiency

... there are noticeable data available for other subtypes. Envelope region of different HIV-1 isolates showed high variability (approximately 30%), and were mainly located in five hypervariable regions (HVRs), V1 to V5. These variations are related to the infidelity of reverse transcriptase enzyme that ...
Vll. Nitrogen metabolism:
Vll. Nitrogen metabolism:

... Vll. Metabolism Glucose, Fatty acids, amino acids: ...
t_tlusty_nodalweek
t_tlusty_nodalweek

... • Messages are written in molecular words that are read and interpreted by other molecules, which calculate the response etc… • Typical energy scale ~ a few kBT. • Thermal noise → errors. • Information channels adapt to errors through evolutionary of selection-mutation • Some errors = mutations are ...
Open access - Bioinformation
Open access - Bioinformation

... Aromatase (CYP19A1) the key enzyme of estrogen biosynthesis, is often deregulated in breast cancer patients. It catalyzes the conversion of androgen to estrogen, thus responsible for production of estrogen in human body. However, it causes over-production of estrogen which eventually leads to prolif ...
L-Arginine A Versatile, Conditionally Essential Amino Acid
L-Arginine A Versatile, Conditionally Essential Amino Acid

MD Simulations of the P53 oncoprotein structure
MD Simulations of the P53 oncoprotein structure

lecture04_05
lecture04_05

... • For aligning amino acids, we need a scoring matrix of 20 rows  20 columns • Matrices represent biological processes – Mutation causes changes in sequence – Evolution tends to conserve protein function – Similar function requires similar amino acids ...
1. The table below refers to some disaccharides, their constituent
1. The table below refers to some disaccharides, their constituent

... Pentose sugar present Is single stranded (Total 4 marks) ...
< 1 ... 132 133 134 135 136 137 138 139 140 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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