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Actin
Actin

... Passes from myofibril to myofibril Attaching the myofilaments to each other across the muscle fiber ...
Theranostics Evolution- and Structure
Theranostics Evolution- and Structure

chapter 11
chapter 11

ppt - pedagogix
ppt - pedagogix

... Some domains are found in many proteins. This does not mean that these proteins have the same function. The width of the alignment should thus be analyzed to assess whether the alignment covers most of the sequence length, or only a small segment. ...
Chapter 2 - University of the Free State
Chapter 2 - University of the Free State

... •At higher salt concentrations, weak interactions between the disordered water molecules are further disrupted, i.e., the molecules become even less ordered •transfer of a water molecule from the ordered environment next to the hydrophobic droplet to the disordered state, will have a larger ∆S compo ...
lec4-5-biosynthesis_specificity
lec4-5-biosynthesis_specificity

... • transported to appropriate cellular location • degraded when no longer needed • Research in ribosome structure reveals that proteins are synthesized by a gigantic RNA enzyme (ribozyme) ...
Topic guide 12.4: Analysis methods
Topic guide 12.4: Analysis methods

... share all of the pinpointed motifs. The challenge she then faces is to discern from the results of these scans which of the matched sequences are genuine members of the protein family. At a given scoring threshold, she will find many cases where the matched sequences have high scores but are not rel ...
Suppression mechanisms
Suppression mechanisms

... cdc2-3w wee1-50 double mutant. The mik1 gene encodes a protein kinase that directly phosphorylates Cdc2 on Tyr15, inhibiting Cdc2 activity. Phosphorylation is a common regulatory modification in vivo, but other types of modifications have also been identified by suppressors. RAM1, for example, was i ...
The dinitrogenase reductase
The dinitrogenase reductase

... Trp, His, glucosamine-6-P, CTP, and AMP. • The amino groups of most other amino acids are derived from glutamate via transamination. ...
Student PPT Notes
Student PPT Notes

...  as substrate/enzyme levels increase, the rxn rate increases until active sites of all enzymes are being continuously occupied by a new substrate  Genes that code for enzymes can turn ________________(i.e. marathon runners after high-carb pre-competition meals)  Some enzymes only synthesized at _ ...
Pipecleaner Proteins Lab
Pipecleaner Proteins Lab

Pipecleaner Proteins Lab
Pipecleaner Proteins Lab

... Because proteins are smaller than microscopic, we would have a pretty hard time doing a hands-on lab on this topic. However, we can explore proteins in an indirect way through modeling. Everything in science is done with models – the scientific method itself is about modeling complex ideas into simp ...
Lac
Lac

... SERUM ALBUMIN (SA) : Serum albumin comes from the serum; it is not synthesized in the mammary gland. It is presumed to enter the milk via "leakage" by the paracellular pathway, or by uptake with other components such as immunoglobulins. There does not seem to be a more specific mechanism of transpor ...
Translation - clemson.edu
Translation - clemson.edu

... 8. What is a codon? Each group of 3 nucleotides 9. What is the start codon that the ribosome looks for? AUG 10. What brings the amino acids to the mRNA strand and ribosome? A tRNA 11. What is the significance of the start codon? It tells the ribosome where to start translating the mRNA. 12. Draw a p ...
The Arabidopsis chloroplast ribosomal protein L21 is
The Arabidopsis chloroplast ribosomal protein L21 is

... To examine the origin of chloroplast L21 protein in different plant species, we compared the amino acid sequences of the conserved core regions. RPL21c is closer to RPL21m (46% identities) than to the chloroplast-encoded L21 protein of M. polymorpha (31%). Using parsimony, neighbor-joining and Fitch ...
From DNA to Protein
From DNA to Protein

...  A cell’s DNA sequence (genes) contains all the information needed to make the molecules of life  Gene expression • A multistep process including transcription and translation, by which genetic information encoded by a gene is converted into a structural or functional part of a cell or body ...
to linear sequence of 20 amino acids.
to linear sequence of 20 amino acids.

Pipe Cleaner Protein Modeling C. Kohn, Waterford WI Name: Hour
Pipe Cleaner Protein Modeling C. Kohn, Waterford WI Name: Hour

Document
Document

...  A cell’s DNA sequence (genes) contains all the information needed to make the molecules of life  Gene expression • A multistep process including transcription and translation, by which genetic information encoded by a gene is converted into a structural or functional part of a cell or body ...
Types and effects of protein variations. Vihinen
Types and effects of protein variations. Vihinen

... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
2-7 Active-Site Geometry
2-7 Active-Site Geometry

User`s Guide - Structural Bioinformatics Group
User`s Guide - Structural Bioinformatics Group

Probing the conformational changes of the yeast mitochondrial ADP
Probing the conformational changes of the yeast mitochondrial ADP

... binding site is accessible to the cytoplasm, whereas in the matrix state, which can be locked by bongkrekic acid, the substrate binding site is open to the mitochondrial matrix. Access to the substrate binding site is regulated by salt bridge networks on either side of the central cavity, called the ...
Nature
Nature

... biogenic amines, from the synapse to the cytoplasm of neurons and glia. These transporters are the targets of therapeutic and illicit compounds, and their dysfunction has been implicated in multiple diseases of the nervous system. Here we present the crystal structure of a bacterial homologue of the ...
One 2-fold axis of symmetry
One 2-fold axis of symmetry

... The diagram provides an alignment of the primary structures of these two domains (H=heavy, L=light), generated by the sort of computer program used in the DNA Cloning laboratory class. Identities are indicated by vertical bars, and residues of greater and lesser chemical similarity are indicated by ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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