An RNA-binding domain in the viral haemorrhagic septicaemia virus
... blue) was added to each. The mixtures were then separated by electrophoresis on 5 % non-denaturing polyacrylamide gels (60 : 1 acrylamide : bisacrylamide) in 0±5¬ TBE (45 mM Tris– borate ; 45 mM boric acid ; 1 mM EDTA) for 5 h at 50 V and 4 °C. Gels were then dried and exposed to X-ray films. Condit ...
... blue) was added to each. The mixtures were then separated by electrophoresis on 5 % non-denaturing polyacrylamide gels (60 : 1 acrylamide : bisacrylamide) in 0±5¬ TBE (45 mM Tris– borate ; 45 mM boric acid ; 1 mM EDTA) for 5 h at 50 V and 4 °C. Gels were then dried and exposed to X-ray films. Condit ...
DR6000 for Quantitative Determination of Proteins
... Quantitative photometric measurements of proteins The quantitative determination of proteins using photometry is often done on the basis of colour reactions of the functional groups of proteins with the corresponding dye reagents. As with the photometric test for individual parameters, the intensity ...
... Quantitative photometric measurements of proteins The quantitative determination of proteins using photometry is often done on the basis of colour reactions of the functional groups of proteins with the corresponding dye reagents. As with the photometric test for individual parameters, the intensity ...
Exam III Answer Key - Weber State University
... 41. Secondly, how do you expect that this change in extracellular sodium might affect an action potential in this neuron should a depolarizing stimulus be received at the neuron cell body? (2 points) Assuming that the cell is not very permeable to sodium while it’s at rest (similar to a typical cell ...
... 41. Secondly, how do you expect that this change in extracellular sodium might affect an action potential in this neuron should a depolarizing stimulus be received at the neuron cell body? (2 points) Assuming that the cell is not very permeable to sodium while it’s at rest (similar to a typical cell ...
Concept review: Chromatography (applied to protein purification)
... Salting Out. Most proteins are less soluble at high salt concentrations, an effect called salting out. The salt concentration at which a protein precipitates differs from one protein to another. Hence, salting out can be used to fractionate proteins. For example, 0.8 M ammonium sulfate precipitates ...
... Salting Out. Most proteins are less soluble at high salt concentrations, an effect called salting out. The salt concentration at which a protein precipitates differs from one protein to another. Hence, salting out can be used to fractionate proteins. For example, 0.8 M ammonium sulfate precipitates ...
CH 460 Dr. Muccio What are the 4 levels of protein structure and
... What are the 4 levels of protein structure and describe each. Do proteins with similar structures usually have similar functions? Primary - amino acid sequence Secondary – helices, sheets, turns, loops Tertiary – 3d folding Quaternary – organization of 3d subunits Yes usually (not always), similar s ...
... What are the 4 levels of protein structure and describe each. Do proteins with similar structures usually have similar functions? Primary - amino acid sequence Secondary – helices, sheets, turns, loops Tertiary – 3d folding Quaternary – organization of 3d subunits Yes usually (not always), similar s ...
Cell-Based Applications of Living Colors® Proteins
... BD™ Pathway Confocal Bioimager. Panel A. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated into the same well and then imaged at 20X magnification using the BD™ Pathway Bioimager. Chroma Technology filter sets were used to separate the signal o ...
... BD™ Pathway Confocal Bioimager. Panel A. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated into the same well and then imaged at 20X magnification using the BD™ Pathway Bioimager. Chroma Technology filter sets were used to separate the signal o ...
No Slide Title
... Neuropeptides synthesized in cytosol sorted/packaged into vesicles for use. (as is dopamine- but not through translation) Protein 2: Neurotransmitter receptors are proteins. Synthesized in cytosol, inserted into ER membrane and sent to proper location on plasma membrane Broad Hypothesis: Perhaps Bip ...
... Neuropeptides synthesized in cytosol sorted/packaged into vesicles for use. (as is dopamine- but not through translation) Protein 2: Neurotransmitter receptors are proteins. Synthesized in cytosol, inserted into ER membrane and sent to proper location on plasma membrane Broad Hypothesis: Perhaps Bip ...
15N Solid-State NMR Study for the Development of a Membrane
... PISEMA S31N M2 TMD on Glass • Superimposed PISEMA spectra of 15NVal28,Ala30,Ile42 S31N M2-TMD in aligned DMPC bilayers on glass slides, with (red) and without (black) drug present • Strong impacts near and far ...
... PISEMA S31N M2 TMD on Glass • Superimposed PISEMA spectra of 15NVal28,Ala30,Ile42 S31N M2-TMD in aligned DMPC bilayers on glass slides, with (red) and without (black) drug present • Strong impacts near and far ...
Document
... DNA Polymerase – a protein complex that copies DNA to DNA RNA Polymerase – a protein complex that copies DNA to RNA Spliceosome – a protein/RNA complex that removes introns from pre-mRNA Ribosome – a protein/RNA complex that translates mRNA codons to amino acids, making proteins Intron – a non-codin ...
... DNA Polymerase – a protein complex that copies DNA to DNA RNA Polymerase – a protein complex that copies DNA to RNA Spliceosome – a protein/RNA complex that removes introns from pre-mRNA Ribosome – a protein/RNA complex that translates mRNA codons to amino acids, making proteins Intron – a non-codin ...
ans - Gogarten Lab
... negative feedback loops that help maintain homeostasis. D. Mars cooled faster than the Earth and therefore was a more habitable place for life earlier. Life arose first on Mars and traveled to Earth on meteorites E. Large glaciers and ice ages result from a runaway cold-house, where the Earth get ...
... negative feedback loops that help maintain homeostasis. D. Mars cooled faster than the Earth and therefore was a more habitable place for life earlier. Life arose first on Mars and traveled to Earth on meteorites E. Large glaciers and ice ages result from a runaway cold-house, where the Earth get ...
Prof. Dr. Harry F. Noller Prof. Dr. Ada Yonath
... Furthermore, the body is a dynamic machine. At all times programmed death of cells occurs, by a process called apoptosis (for the discovery of which the Paul Ehrlich and Ludwig Darmstaedter Prize 2000 was awarded to Drs. Kerr and Horvitz) and simultaneously there is a constant production of new cell ...
... Furthermore, the body is a dynamic machine. At all times programmed death of cells occurs, by a process called apoptosis (for the discovery of which the Paul Ehrlich and Ludwig Darmstaedter Prize 2000 was awarded to Drs. Kerr and Horvitz) and simultaneously there is a constant production of new cell ...
Table S1. Entities that were analyzed in the pathways shown in
... Table S1. Entities that were analyzed in the pathways shown in Figure 4 Name CD24 ...
... Table S1. Entities that were analyzed in the pathways shown in Figure 4 Name CD24 ...
Bottom-up Nanobiotechnology
... function combinatorially to allow the brain to discriminate odors ...
... function combinatorially to allow the brain to discriminate odors ...
Detecting topological patterns in protein networks
... cond-mat/0207366, (2002) published in PRL (2002) Followed by Deeds, E.J. and Ashenberg, O. and Shakhnovich, E.I., A simple physical model for scaling in protein-protein interaction networks, PNAS (2006) Then others including Yi Y. Shi, G.A. Miller, H. Qian, and K. Bomsztyk, Free-energy distribution ...
... cond-mat/0207366, (2002) published in PRL (2002) Followed by Deeds, E.J. and Ashenberg, O. and Shakhnovich, E.I., A simple physical model for scaling in protein-protein interaction networks, PNAS (2006) Then others including Yi Y. Shi, G.A. Miller, H. Qian, and K. Bomsztyk, Free-energy distribution ...
PURExpress® Δ Ribosome Kit
... PURExpress® is based on the PURE System Technology originally developed by Dr. Takuya Ueda at the University of Tokyo and commercialized as the PURESYSTEM® by BioComber (Tokyo, Japan). Licensed from BioComber (Tokyo, Japan) under Patent Nos. 7,118,883; WO2005-105994 and JP2006-340694. For research u ...
... PURExpress® is based on the PURE System Technology originally developed by Dr. Takuya Ueda at the University of Tokyo and commercialized as the PURESYSTEM® by BioComber (Tokyo, Japan). Licensed from BioComber (Tokyo, Japan) under Patent Nos. 7,118,883; WO2005-105994 and JP2006-340694. For research u ...
No Slide Title
... HMMTOP - Prediction of transmembrane helices and topology of proteins (Hungarian Academy of Sciences) PredictProtein - Prediction of transmembrane helix location and topology (Columbia University) SOSUI - Prediction of transmembrane regions (TUAT; Tokyo Univ. of Agriculture & Technology) TMAP - ...
... HMMTOP - Prediction of transmembrane helices and topology of proteins (Hungarian Academy of Sciences) PredictProtein - Prediction of transmembrane helix location and topology (Columbia University) SOSUI - Prediction of transmembrane regions (TUAT; Tokyo Univ. of Agriculture & Technology) TMAP - ...
Human CAMP/LL37/FALL39 Antibody
... blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Sheep AntiHuman CAMP/LL37/FALL39 Antigen Affinitypurified Polyclonal Antibody (Catalog # AF7497) followed by HRPconjugated AntiSheep IgG Secondary Antibody (Catalog # HAF016). A sp ...
... blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Sheep AntiHuman CAMP/LL37/FALL39 Antigen Affinitypurified Polyclonal Antibody (Catalog # AF7497) followed by HRPconjugated AntiSheep IgG Secondary Antibody (Catalog # HAF016). A sp ...
RER - Botanik in Bonn
... • Pen E J , Heinlein M: Cortical microtubule-associated ER sites: organization centers of cell polarity and communication. Curr Opin ...
... • Pen E J , Heinlein M: Cortical microtubule-associated ER sites: organization centers of cell polarity and communication. Curr Opin ...
Lesson One: The Four Basic Food Molecules
... shapes. Two important shapes are the alpha helix and the beta pleated sheet. Proteins are often denatured by heat or acid. Proteins can coagulate. Enzymes are a specific type of proteins. Fats and Oils The major constituent of natural fats and oils is the triglyceride, a combination of three fatty a ...
... shapes. Two important shapes are the alpha helix and the beta pleated sheet. Proteins are often denatured by heat or acid. Proteins can coagulate. Enzymes are a specific type of proteins. Fats and Oils The major constituent of natural fats and oils is the triglyceride, a combination of three fatty a ...
lecture 10
... ‘OH’ denotes hydroxylated residues ‘+’ denotes positively charged aa’s most signals are at the N-terminus can be cryptic ...
... ‘OH’ denotes hydroxylated residues ‘+’ denotes positively charged aa’s most signals are at the N-terminus can be cryptic ...
Anti-Heat Shock Protein 27 (HSP27) Developed in Rabbit, IgG
... by various cytokines, growth factors, hormones and chemicals. HSP27 shows a rapid phosphorylation, following exposure to stress stimuli.4,5 It is phosphorylated on multiple serine residues by MAPKAP kinase 2/3 in the p38 MAPK stresssensitive signaling pathway.4-7 HSP27 acts as an actin-cap binding p ...
... by various cytokines, growth factors, hormones and chemicals. HSP27 shows a rapid phosphorylation, following exposure to stress stimuli.4,5 It is phosphorylated on multiple serine residues by MAPKAP kinase 2/3 in the p38 MAPK stresssensitive signaling pathway.4-7 HSP27 acts as an actin-cap binding p ...
Unit 2, Test 1 Review
... Therefore, the solution is _______ when compared to a cell with a low amount of dissolved solutes. ...
... Therefore, the solution is _______ when compared to a cell with a low amount of dissolved solutes. ...
Protein and proteome analytics
... Sensitive detection of the ingredients by means of MALDI-MS Comparative analyses of different samples by means of a principal component analysis to determine the differences Identification of the proteins by means of MS/MS (PSD or ...
... Sensitive detection of the ingredients by means of MALDI-MS Comparative analyses of different samples by means of a principal component analysis to determine the differences Identification of the proteins by means of MS/MS (PSD or ...
Master Dissertation Project
... Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), despite being completely curable, has reemerged as a global pandemic. The failure of effective vaccine protection, lack of early detection of the disease, emergence of drug resistance and the deadly synergism with HIV infection have lim ...
... Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), despite being completely curable, has reemerged as a global pandemic. The failure of effective vaccine protection, lack of early detection of the disease, emergence of drug resistance and the deadly synergism with HIV infection have lim ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.