A proteomic study of African elephant milk: Inter
... DNA methylation at specific sites on the αs1-casein promoter was able to down regulate the expression of αs1-casein during mammary gland involution (Singh et al., 2008). Since milk proteins are secretory proteins, they have to be exported into the milk pool in the alveolar lumen. Milk protein transp ...
... DNA methylation at specific sites on the αs1-casein promoter was able to down regulate the expression of αs1-casein during mammary gland involution (Singh et al., 2008). Since milk proteins are secretory proteins, they have to be exported into the milk pool in the alveolar lumen. Milk protein transp ...
Comparison of the activities of protein disulphide
... the assay was then converted into the quantity of RNAase in the PDI incubation by a factor F, which depended on the volumes used. When the assay sample (A ,ll) was withdrawn from the PDI incubation (original volume V,ul) and assayed in the cuvette (C ,tl), F = CV/A. Thus I ,umol of RNAase generated ...
... the assay was then converted into the quantity of RNAase in the PDI incubation by a factor F, which depended on the volumes used. When the assay sample (A ,ll) was withdrawn from the PDI incubation (original volume V,ul) and assayed in the cuvette (C ,tl), F = CV/A. Thus I ,umol of RNAase generated ...
Answers - U of L Class Index
... The inhibitor in competitive reversible inhibition competes for the active site because it has a structure similar to the substrate. Increasing the substrate concentration reverses the inhibition. The inhibitor in noncompetitive inhibition is not similar to the substrate and does not compete for the ...
... The inhibitor in competitive reversible inhibition competes for the active site because it has a structure similar to the substrate. Increasing the substrate concentration reverses the inhibition. The inhibitor in noncompetitive inhibition is not similar to the substrate and does not compete for the ...
Aspects of Reductive Explanation in Biological Science: Intrinsicality
... applicable if not also relevant to current research. The philosophical task is to explicate the reasoning in particular areas of biology and understand the diverse standards used by scientists to assess whether reductive explanations are successful, not to be for or against reductionism per se. Redu ...
... applicable if not also relevant to current research. The philosophical task is to explicate the reasoning in particular areas of biology and understand the diverse standards used by scientists to assess whether reductive explanations are successful, not to be for or against reductionism per se. Redu ...
Bioinformatics
... proteins: easier to detect patterns • Searches with DNA sequences produce fewer significant matches • What if you don’t know reading frame? • Sometimes must do nucleic acid searches (searching for similarities in non-coding regions) ...
... proteins: easier to detect patterns • Searches with DNA sequences produce fewer significant matches • What if you don’t know reading frame? • Sometimes must do nucleic acid searches (searching for similarities in non-coding regions) ...
figure 18.2
... phosphorylation. LRP5/6 phosphorylation prevents phosphorylation of β-catenin and thereby its degradation. Subsequently β-catenin accumulates in the cytoplasm and enters the nucleus where it interacts with Tcf/Lef transcription factors and recruits coactivators, such as CBP/p300, to initiate gene tr ...
... phosphorylation. LRP5/6 phosphorylation prevents phosphorylation of β-catenin and thereby its degradation. Subsequently β-catenin accumulates in the cytoplasm and enters the nucleus where it interacts with Tcf/Lef transcription factors and recruits coactivators, such as CBP/p300, to initiate gene tr ...
subset seed extension to protein blast
... thus too large for everyday tasks, most of sequence aligning is done using heuristics, typically with the ubiquitous BLAST software (Altschul et al., 1990; Altschul et al., 1997). It runs in three phases, and the first of which finds short initial alignments, so called hot spots. Theory of seeds may ...
... thus too large for everyday tasks, most of sequence aligning is done using heuristics, typically with the ubiquitous BLAST software (Altschul et al., 1990; Altschul et al., 1997). It runs in three phases, and the first of which finds short initial alignments, so called hot spots. Theory of seeds may ...
Introduction
... Results of many color reactions depend on the reactive groups in side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produ ...
... Results of many color reactions depend on the reactive groups in side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produ ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
... activation of the amino acid’s α-carboxyl group by reaction with ATP. In cells, activation and aminoacylation require a separate enzyme for each amino acid. These assignment catalysts, called aminoacyl-tRNA synthetases (aaRS), were first clearly identified by Berg and Ofe ...
... activation of the amino acid’s α-carboxyl group by reaction with ATP. In cells, activation and aminoacylation require a separate enzyme for each amino acid. These assignment catalysts, called aminoacyl-tRNA synthetases (aaRS), were first clearly identified by Berg and Ofe ...
STING Millennium: a web-based suite of programs
... rows indicate the secondary structure according to the annotations in PDB, DSSP and Stride, respectively. Another five rows are displayed color coded according to: temperature factor, relative entropy, accessibility in complex, accessibility in isolation and dihedral angles. His_57 and parameters be ...
... rows indicate the secondary structure according to the annotations in PDB, DSSP and Stride, respectively. Another five rows are displayed color coded according to: temperature factor, relative entropy, accessibility in complex, accessibility in isolation and dihedral angles. His_57 and parameters be ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
... activation of the amino acid’s α-carboxyl group by reaction with ATP. In cells, activation and aminoacylation require a separate enzyme for each amino acid. These assignment catalysts, called aminoacyl-tRNA synthetases (aaRS), were first clearly identified by Berg and Ofe ...
... activation of the amino acid’s α-carboxyl group by reaction with ATP. In cells, activation and aminoacylation require a separate enzyme for each amino acid. These assignment catalysts, called aminoacyl-tRNA synthetases (aaRS), were first clearly identified by Berg and Ofe ...
Sixth Southeast Enzyme Conference
... same intermediate (FeIII-O2•-[MYW]•+) at 10 ms, but later, at the point of H2O2 depletion, a broad exchange-coupled radical was observed for wild-type but not W321F. The properties of the radical were consistent with a FeIV=O W321•+ state and would account for the subsequent accumulation of an inact ...
... same intermediate (FeIII-O2•-[MYW]•+) at 10 ms, but later, at the point of H2O2 depletion, a broad exchange-coupled radical was observed for wild-type but not W321F. The properties of the radical were consistent with a FeIV=O W321•+ state and would account for the subsequent accumulation of an inact ...
Modulation of the Oligomerization State of the Bovine F1
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
Combining docking and molecular dynamic simulations in drug design
... The approaches and methodologies used in drug design have changed over time, exploiting and driving new technological advances to solve the varied bottlenecks found along the way. While until the 90s, the major issues were lead discovery and chemical synthesis of drug-like molecules, the emergence o ...
... The approaches and methodologies used in drug design have changed over time, exploiting and driving new technological advances to solve the varied bottlenecks found along the way. While until the 90s, the major issues were lead discovery and chemical synthesis of drug-like molecules, the emergence o ...
Read more... - Bonanza Calf Nutrition
... “The powder smells like proper milk, the calves absolutely love it. We have had no difficulties with intakes when the calves switch to Shine from cow’s milk.’’ Captal Farms has developed a simple management system to allow calves to thrive. Groups of 40 have access to a 50-teat feeder as this ensure ...
... “The powder smells like proper milk, the calves absolutely love it. We have had no difficulties with intakes when the calves switch to Shine from cow’s milk.’’ Captal Farms has developed a simple management system to allow calves to thrive. Groups of 40 have access to a 50-teat feeder as this ensure ...
Field Guide to Protein Folds
... The bromodomain is central to epigenetic control of gene transcription through its role in acetylating histone lysine. The structure adopts a conserved left-handed bundle of four α helices, with two interhelical loops of variable length and sequence between the first and second and third and fourth ...
... The bromodomain is central to epigenetic control of gene transcription through its role in acetylating histone lysine. The structure adopts a conserved left-handed bundle of four α helices, with two interhelical loops of variable length and sequence between the first and second and third and fourth ...
Biomarker discovery for psychiatric disorders
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
... Although over the last century tremendous progress has been made in the therapy and mortality decrease of devastating conditions such as cancer and cardiopathies, no decrease has been observed in mortality rates or overall prevalence of psychiatric disorders (Kessler et al., 2005). It is therefore n ...
Chromatography - Taleem Network
... affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. ...
... affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. ...
Protein Creation Pathway
... Remember, we are tracing the path of protein creation. 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
... Remember, we are tracing the path of protein creation. 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
Unit: Enzymes I
... The activity of an enzyme may be measured by two different approaches: fixed time (two-point assay) and continuous monitoring (Kinetic assay). Two-point Assays The first enzyme tests commonly employed in the clinical laboratory (amylase, lipase, alkaline and acid phosphatase) utilized a fixed time f ...
... The activity of an enzyme may be measured by two different approaches: fixed time (two-point assay) and continuous monitoring (Kinetic assay). Two-point Assays The first enzyme tests commonly employed in the clinical laboratory (amylase, lipase, alkaline and acid phosphatase) utilized a fixed time f ...
... (right) that affect drug binding . Circle the residue and briefly justify your answer (2 pts). ii) Select one residue that is responsible for catalytic activity and describe how replacement of that residue with glycine (i.e. removal of the sidechain) would affect the catalytic mechanism (4 pts) iii) ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.