SUC1 and SUC2: two sucrose transporters from Arabidopsis
... PMAI gene containing a unique EcoRI cloning site (Figure 3). This vector allows direct insertion of SUC1 and SUC2 cDNAs. The PMA 1 promoter was chosen because it is very active in glucose-grown yeast cells, and the H÷ATPase is one of the most prominent proteins of the plasma membrane. Figure 4 shows ...
... PMAI gene containing a unique EcoRI cloning site (Figure 3). This vector allows direct insertion of SUC1 and SUC2 cDNAs. The PMA 1 promoter was chosen because it is very active in glucose-grown yeast cells, and the H÷ATPase is one of the most prominent proteins of the plasma membrane. Figure 4 shows ...
File Ref.No.7054/GA - IV - J1/2013/CU UNIVERSITY OF CALICUT
... omission of model question paper in the approved syllabus. The Chairman, Board of studies has forwarded the model question papers. The Registrar has approved to incorporate the model question papers to the syllabus of BSc Biochemisty as per the order cited 8th. Therefore the Scheme and Syllabus impl ...
... omission of model question paper in the approved syllabus. The Chairman, Board of studies has forwarded the model question papers. The Registrar has approved to incorporate the model question papers to the syllabus of BSc Biochemisty as per the order cited 8th. Therefore the Scheme and Syllabus impl ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... forward (deliberate modification of biological systems) ...
... forward (deliberate modification of biological systems) ...
Cytochromes P450 – importance of tissue specificity
... 14α-demetylase (CYP51) is one example of recycling between ER and cis-Golgi in somatic cells [14, 15] and transport through Golgi to the acrosome in male germ cells [15]. The exact reason for the static retention or recycling of cytochromes P450 is not fully understood. ER resident proteins must pos ...
... 14α-demetylase (CYP51) is one example of recycling between ER and cis-Golgi in somatic cells [14, 15] and transport through Golgi to the acrosome in male germ cells [15]. The exact reason for the static retention or recycling of cytochromes P450 is not fully understood. ER resident proteins must pos ...
Chapter 26:Biomolecules: Amino Acids, Peptides, and Proteins
... congregate on the exterior of the protein where they can be solvated by water Amino acids with neutral, nonpolar side chains congregate on the hydrocarbon-like interior of a protein molecule Also important for stabilizing a protein's tertiary structure are the formation of disulfide bridges betw ...
... congregate on the exterior of the protein where they can be solvated by water Amino acids with neutral, nonpolar side chains congregate on the hydrocarbon-like interior of a protein molecule Also important for stabilizing a protein's tertiary structure are the formation of disulfide bridges betw ...
Engineering subunit association of multisubunit proteins
... of BL21(DE3)(pLysE) carrying pTSA-33, which had been incubated for 4 hr after induction, was prepared and then treated with DNase I and RNase A. An inclusion body (insoluble) fraction was collected by centrifugation of the cell lysate and dissolved in 7 M guanidine hydrochloride, pH 1.5. The dissolv ...
... of BL21(DE3)(pLysE) carrying pTSA-33, which had been incubated for 4 hr after induction, was prepared and then treated with DNase I and RNase A. An inclusion body (insoluble) fraction was collected by centrifugation of the cell lysate and dissolved in 7 M guanidine hydrochloride, pH 1.5. The dissolv ...
spectroscopic studies of mosquito iridescent virus, its capsid
... Optical spectroscopy is one of the efficient and informative methods of studying viruses. It provides well reproducible results and is a powerful supplement to conventional biological methods of studying the living organisms. Thus, it allows one to determine the chemical composition of the capsid pr ...
... Optical spectroscopy is one of the efficient and informative methods of studying viruses. It provides well reproducible results and is a powerful supplement to conventional biological methods of studying the living organisms. Thus, it allows one to determine the chemical composition of the capsid pr ...
Kinetic analysis of cooperativity of phosphorylated L
... calculated from Eq. (10). As the simultaneous influence of both substrates on the reaction rate was taken into account by this model, these parameters do not depend on the concentration of either of the two substrates. Therefore the constant K A should explicitly characterize the interaction of ADP ...
... calculated from Eq. (10). As the simultaneous influence of both substrates on the reaction rate was taken into account by this model, these parameters do not depend on the concentration of either of the two substrates. Therefore the constant K A should explicitly characterize the interaction of ADP ...
... protein source for vegetarians as well as animal feed. The search for an alternative source of plant protein is important to cut down the expenditure, reduce protein energy malnutrition and to maintain the animal production as well. Amino acid analysis is a good indicator to measure the quality of p ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
Development of Software Package for Determining Protein
... Removing unfavorable van der Waals clashes (when two atoms try to occupy the same space) Assigning charge ( partial or whole) and van der Waals radii parameters from a variety of force fields ...
... Removing unfavorable van der Waals clashes (when two atoms try to occupy the same space) Assigning charge ( partial or whole) and van der Waals radii parameters from a variety of force fields ...
Markers of renal function tests (PDF Available)
... healthy children and those with mild and advanced renal disease. In one of the study the mean renal extraction of Cystatin C was equal to the mean renal extraction of 125 iodine (I)-iothalamate in hypertensive patients, suggesting tubular secretion of Cystatin C [25]. It was possible to get an accur ...
... healthy children and those with mild and advanced renal disease. In one of the study the mean renal extraction of Cystatin C was equal to the mean renal extraction of 125 iodine (I)-iothalamate in hypertensive patients, suggesting tubular secretion of Cystatin C [25]. It was possible to get an accur ...
Chapter 2: Biochemistry Problems
... In these problems, you will be given the covalent bonds (these are shown as solid lines) and must infer their noncovalent bonding properties. Noncovalent bonds/interactions are shown by dotted lines (etc.). These two types of “bonds” are entirely separate; for example, an oxygen (which can make only ...
... In these problems, you will be given the covalent bonds (these are shown as solid lines) and must infer their noncovalent bonding properties. Noncovalent bonds/interactions are shown by dotted lines (etc.). These two types of “bonds” are entirely separate; for example, an oxygen (which can make only ...
Nucleus-Encoded Genes for Plastid
... isoprenoids, and heme by the use of plastid-targeted enzymes. To study a completely independent transformation of a photosynthetic plastid to a cryptic plastid in another alga-turned-parasite, we conducted an expressed sequence tag (EST) survey of Helicosporidium. This parasite has recently been rec ...
... isoprenoids, and heme by the use of plastid-targeted enzymes. To study a completely independent transformation of a photosynthetic plastid to a cryptic plastid in another alga-turned-parasite, we conducted an expressed sequence tag (EST) survey of Helicosporidium. This parasite has recently been rec ...
Structure and mechanism of action of a novel
... (Chander et al., 1998). These latter organisms include members of Staphylococcus and Streptococcus species, some of which are important human pathogens. Consequently, it is possible that iPGMs could be a target for rational design of a novel antibiotic. Interestingly, some bacteria have genes for bo ...
... (Chander et al., 1998). These latter organisms include members of Staphylococcus and Streptococcus species, some of which are important human pathogens. Consequently, it is possible that iPGMs could be a target for rational design of a novel antibiotic. Interestingly, some bacteria have genes for bo ...
Feline IFN-γ Antibody
... Interferongamma (IFNγ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFNγ exists as a noncovalently linked homodimer of 20 25 kDa variably glycosylated subunits ...
... Interferongamma (IFNγ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFNγ exists as a noncovalently linked homodimer of 20 25 kDa variably glycosylated subunits ...
eg1
... Compared with other cultivated edible mushrooms such as Agaricus bisporus, Lentinula edodes and Pleurotus sajor-caju, the biological efficiency of V. volvacea (i.e. conversion of growth substrate into mushroom fruit bodies) is very low (Chang 1974). This effect appears to be related to the inability ...
... Compared with other cultivated edible mushrooms such as Agaricus bisporus, Lentinula edodes and Pleurotus sajor-caju, the biological efficiency of V. volvacea (i.e. conversion of growth substrate into mushroom fruit bodies) is very low (Chang 1974). This effect appears to be related to the inability ...
BEL β-trefoil: A novel lectin with antineoplastic properties in king
... called lectins (Sharon 2007). In general, ligand binding precedes the fulfillment of an important biological function, which in some cases is still not known. Some members of this family are also called agglutinins because of their ability to agglutinate red blood cells, but this term does not necess ...
... called lectins (Sharon 2007). In general, ligand binding precedes the fulfillment of an important biological function, which in some cases is still not known. Some members of this family are also called agglutinins because of their ability to agglutinate red blood cells, but this term does not necess ...
Critically discuss the methods that are in routine use for the
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
Tendency for Local Repetitiveness in Amino Acid Usages in Modern
... they have a repetitiveness score ( 1.15 or above) signi®cantly higher than 1.0 even at far distal locations. Although we have not investigated the cause as yet, one possibility may be that amino acid composition is different among the neural proteins, because some (e.g. ion channels) contain many h ...
... they have a repetitiveness score ( 1.15 or above) signi®cantly higher than 1.0 even at far distal locations. Although we have not investigated the cause as yet, one possibility may be that amino acid composition is different among the neural proteins, because some (e.g. ion channels) contain many h ...
ab83376 Phenylalanine Assay Kit
... concentrations). For serum samples, the dilution is to 76% so divide assay values by 0.76 to correct values to original sample concentrations. b) For further analysis of samples, if assay buffer is 0.1M or stronger, samples up to 50 µl may be used directly in 100 µl assay reactions. If lower concent ...
... concentrations). For serum samples, the dilution is to 76% so divide assay values by 0.76 to correct values to original sample concentrations. b) For further analysis of samples, if assay buffer is 0.1M or stronger, samples up to 50 µl may be used directly in 100 µl assay reactions. If lower concent ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... Multiple Reaction Monitoring (MRM), also known as Selected Reaction Monitoring (SRM), is a powerful method for performing targeted proteomics. The approach is based on the generation of specific quantitative assays for each protein of interest and can be used to accurately quantitate large sets of p ...
... Multiple Reaction Monitoring (MRM), also known as Selected Reaction Monitoring (SRM), is a powerful method for performing targeted proteomics. The approach is based on the generation of specific quantitative assays for each protein of interest and can be used to accurately quantitate large sets of p ...
Test Example
... (a) List the types of noncovalent interactions that are important in providing stability to the threedimensional structures of macromolecules. (b) Why is it important that these interactions be noncovalent, rather than covalent, bonds? Ans: (a) Noncovalent interactions include hydrogen bonds, ionic ...
... (a) List the types of noncovalent interactions that are important in providing stability to the threedimensional structures of macromolecules. (b) Why is it important that these interactions be noncovalent, rather than covalent, bonds? Ans: (a) Noncovalent interactions include hydrogen bonds, ionic ...
Protein Creation Pathway
... 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
... 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
Protein Creation Pathway
... 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
... 1st step: The nucleolus creates ribosomes 2nd step: The ribosomes exit the nucleus 3rd step: The ribosomes begin to make proteins as they travel along the rough ER. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.