Post-doctoral Position in Macromolecular Crystallography
... purification who want to learn structural biology techniques. X-ray crystallography resources at UTMB include two X-ray area detector systems, a Phoenix crystallization robot, a Minstrel crystal imaging robot, and an Alchemist liquid handling robot. Additionally, as a member of the Gulf Coast Protei ...
... purification who want to learn structural biology techniques. X-ray crystallography resources at UTMB include two X-ray area detector systems, a Phoenix crystallization robot, a Minstrel crystal imaging robot, and an Alchemist liquid handling robot. Additionally, as a member of the Gulf Coast Protei ...
doc BIOL210syllabus
... The two photosystems and photosynthetic electron transport CO2 fixation: the dark reactions of the Calvin cycle Lodish et al., pp. 511-529. Lecture 15. Protein targeting to the endoplasmic reticulum I. February 5. How proteins are targeted to specific locations in the cell An overview of the ...
... The two photosystems and photosynthetic electron transport CO2 fixation: the dark reactions of the Calvin cycle Lodish et al., pp. 511-529. Lecture 15. Protein targeting to the endoplasmic reticulum I. February 5. How proteins are targeted to specific locations in the cell An overview of the ...
Biology 177-201B
... The two photosystems and photosynthetic electron transport CO2 fixation: the dark reactions of the Calvin cycle Lodish et al., pp. 511-529. Lecture 15. Protein targeting to the endoplasmic reticulum I. February 5. How proteins are targeted to specific locations in the cell An overview of the ...
... The two photosystems and photosynthetic electron transport CO2 fixation: the dark reactions of the Calvin cycle Lodish et al., pp. 511-529. Lecture 15. Protein targeting to the endoplasmic reticulum I. February 5. How proteins are targeted to specific locations in the cell An overview of the ...
SP7+ P7 (1+3) Energetics and kinetics of chemical reaction.
... L26 (1) The pentose phosphate pathway. SB26 (1) Other pathways of hexose ...
... L26 (1) The pentose phosphate pathway. SB26 (1) Other pathways of hexose ...
Chapter 5 - My Teacher Site
... • 2) A dehydration reaction joins 2 glucose molecules to form maltose. The formula for glucose is C6H12O6. What is the formula for maltose? • 3) What would happen if a cow were given antibiotics ...
... • 2) A dehydration reaction joins 2 glucose molecules to form maltose. The formula for glucose is C6H12O6. What is the formula for maltose? • 3) What would happen if a cow were given antibiotics ...
Bioch-301.NMR.2 - Vanderbilt Center for Structural Biology
... Proteins Have Too Many Signals! 1H ...
... Proteins Have Too Many Signals! 1H ...
Caulobacter Export™ Manual
... amounts of plasmid from us, it is common to introduce the plasmid into an E. coli strain (e.g., DH5alpha, Top10F’), and then do a standard plasmid preparation, using any of the commercial kits, to obtain useful amounts of plasmid for your work. You will discover that yields are comparable to other s ...
... amounts of plasmid from us, it is common to introduce the plasmid into an E. coli strain (e.g., DH5alpha, Top10F’), and then do a standard plasmid preparation, using any of the commercial kits, to obtain useful amounts of plasmid for your work. You will discover that yields are comparable to other s ...
Biochemistry Review Test
... 1 ml of peroxide. The gas that was generated was collected. A glowing splint burst into flames when placed in the gas. The student then repeated the procedure, using one gram of boiled liver and one gram of liver treated with a strong acid. When peroxide was added to each sample of liver, no gas was ...
... 1 ml of peroxide. The gas that was generated was collected. A glowing splint burst into flames when placed in the gas. The student then repeated the procedure, using one gram of boiled liver and one gram of liver treated with a strong acid. When peroxide was added to each sample of liver, no gas was ...
TRIDIA™ In Vitro Diagnostic Surfaces
... TRIDIA™ In Vitro Diagnostic Surfaces The Success of Your Application Lies in its Surface The interface between non-biological solid supports and sensitive biological capture analytes is at the core of diagnostic assays. Advanced surface coatings from SurModics IVD optimize DNA, RNA, protein, and ce ...
... TRIDIA™ In Vitro Diagnostic Surfaces The Success of Your Application Lies in its Surface The interface between non-biological solid supports and sensitive biological capture analytes is at the core of diagnostic assays. Advanced surface coatings from SurModics IVD optimize DNA, RNA, protein, and ce ...
TMEM65 is a mitochondrial inner-membrane protein
... Immunoblot analysis using anti-TMEM65 antibody showed that the band observed in the control sample (approximately 21 kDa) was absent in the samples prepared from the cells treated with siRNAs, suggesting that the approximately 21-kDa band corresponded to endogenous TMEM65 (Fig. 1C). Therefore, we hy ...
... Immunoblot analysis using anti-TMEM65 antibody showed that the band observed in the control sample (approximately 21 kDa) was absent in the samples prepared from the cells treated with siRNAs, suggesting that the approximately 21-kDa band corresponded to endogenous TMEM65 (Fig. 1C). Therefore, we hy ...
Characterization of Rice Group 3 LEA Genes in Developmental
... the 20-day-old rice seedlings with 20-μM ABA treatment for 12 h were used. For ABA, NaCl, and cold treatment, samples collected from four development stages (seedling, tillering, heading, and seed maturation) were incubated in shaking buffer (1 mM potassium phosphate, 1 % sucrose, 50 μg/ml chloramph ...
... the 20-day-old rice seedlings with 20-μM ABA treatment for 12 h were used. For ABA, NaCl, and cold treatment, samples collected from four development stages (seedling, tillering, heading, and seed maturation) were incubated in shaking buffer (1 mM potassium phosphate, 1 % sucrose, 50 μg/ml chloramph ...
Chapter 7 Review Sheet
... 1. Describe the structure of a phospholipid in detail and be able to draw it. Just use R for the group attached to the phosphate, but do not forget the charge. 2. Indicate how phospholipids align relative to each other in an aqueous solution (we discussed two ways), explain WHY they do this (water c ...
... 1. Describe the structure of a phospholipid in detail and be able to draw it. Just use R for the group attached to the phosphate, but do not forget the charge. 2. Indicate how phospholipids align relative to each other in an aqueous solution (we discussed two ways), explain WHY they do this (water c ...
Lecture 1
... • 1850’s: First amino acids isolated from natural products • 1903-1906: By hydrolysis of natural proteins, Emil Fischer proves that they are copolymers of amino acids (strange, but none of his so fundamental papers earned more than ~60 citations!). • 1930’s and 1940’s: proteins are viewed as spheroi ...
... • 1850’s: First amino acids isolated from natural products • 1903-1906: By hydrolysis of natural proteins, Emil Fischer proves that they are copolymers of amino acids (strange, but none of his so fundamental papers earned more than ~60 citations!). • 1930’s and 1940’s: proteins are viewed as spheroi ...
Structure of living matter.
... the 18S rRNA as spheres and colored according to each domain (5′ domain, red; central domain, green; 3′ major domain, yellow; 3′ minor domain, blue; ESs, magenta), and the proteins as gray cartoons (abbreviations: H, head; Be, beak; N, neck; P, platform; Sh, shoulder; Bo, body; RF, right foot; LF, l ...
... the 18S rRNA as spheres and colored according to each domain (5′ domain, red; central domain, green; 3′ major domain, yellow; 3′ minor domain, blue; ESs, magenta), and the proteins as gray cartoons (abbreviations: H, head; Be, beak; N, neck; P, platform; Sh, shoulder; Bo, body; RF, right foot; LF, l ...
Recombinant DNA Technology Manipulation of Gene Expression in
... molecule or Antibody (Ab) • Small molecule or Ab can be linked to inert matrix • Fusion protein will bind, other proteins won’t • Elute purified protein from column ...
... molecule or Antibody (Ab) • Small molecule or Ab can be linked to inert matrix • Fusion protein will bind, other proteins won’t • Elute purified protein from column ...
Erlanson et al. PNAS - UCSF Macromolecular Structure Group
... of the disulfide library members is present at ⬇0.2 mM, and thus the total concentration of all disulfide library members is ⬇2 mM. The reaction is done in a buffer containing 25 mM potassium phosphate (pH 7.5) and 1 mM 2-mercaptoethanol, although other buffers and reducing agents can be used. The r ...
... of the disulfide library members is present at ⬇0.2 mM, and thus the total concentration of all disulfide library members is ⬇2 mM. The reaction is done in a buffer containing 25 mM potassium phosphate (pH 7.5) and 1 mM 2-mercaptoethanol, although other buffers and reducing agents can be used. The r ...
Cell Biology Part II Notes
... Osmosis – the passive movement of water across a semi-permeable membrane from an area of high concentration to an area of low concentration ...
... Osmosis – the passive movement of water across a semi-permeable membrane from an area of high concentration to an area of low concentration ...
Primary Structure of Proteins
... A thyroid hormone that stimulates the release of thyroxin is a tripeptide with the amino acid sequence Glu–His–Pro, EHP. Although other amino acid sequences of these three amino acids are possible, only the specific sequence or primary structure of Glu−His−Pro produces hormonal activity. ...
... A thyroid hormone that stimulates the release of thyroxin is a tripeptide with the amino acid sequence Glu–His–Pro, EHP. Although other amino acid sequences of these three amino acids are possible, only the specific sequence or primary structure of Glu−His−Pro produces hormonal activity. ...
Protein import into yeast mitochondria van Wilpe, S.
... fragmentss harbouring the mutant genes by PCR. With chromosomal DNA isolated from the timltiml 7 mutants and the parent strain MB3 as a template and primers specific for the flanking regionss of TIM17, a 778 bp PCR fragment harbouring the entire TIM17 gene was obtained. This fragmentfragment contain ...
... fragmentss harbouring the mutant genes by PCR. With chromosomal DNA isolated from the timltiml 7 mutants and the parent strain MB3 as a template and primers specific for the flanking regionss of TIM17, a 778 bp PCR fragment harbouring the entire TIM17 gene was obtained. This fragmentfragment contain ...
Periodicities in Sequence Residue Hydropathy and the Implications on Protein Folds
... In the remainder of this paper, I’ll present the experiment in which I tested for correlation between the sequence periodicities of structurally similar proteins. In order to define the problem at hand, we need to first represent each amino acid as a numerical value. The problems that we face in thi ...
... In the remainder of this paper, I’ll present the experiment in which I tested for correlation between the sequence periodicities of structurally similar proteins. In order to define the problem at hand, we need to first represent each amino acid as a numerical value. The problems that we face in thi ...
Lesson Overview - Midland Park School
... consists of three-letters (or bases in the mRNA molecule). It is known as a codon. A codon consists of three consecutive bases that specify a single amino acid to be added to the polypeptide chain. ...
... consists of three-letters (or bases in the mRNA molecule). It is known as a codon. A codon consists of three consecutive bases that specify a single amino acid to be added to the polypeptide chain. ...
Histidine protonation and the activation of viral fusion proteins
... residues. Furthermore, homology mapping of the aligned sequences on to the X-ray crystal structure of the pre-fusion DEN2 (dengue virus type 2) sE (soluble E) protein (1OKE [10]; Figure 1) showed that although certain histidine residues were not fully conserved they were compensated by other mutatio ...
... residues. Furthermore, homology mapping of the aligned sequences on to the X-ray crystal structure of the pre-fusion DEN2 (dengue virus type 2) sE (soluble E) protein (1OKE [10]; Figure 1) showed that although certain histidine residues were not fully conserved they were compensated by other mutatio ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.