Glycosylation of Antibodies - Department of Chemistry
... The initial analysis was to determine the amount of neutral sugars and sialic acid forms using a phenol-sulfuric acid method and RP-HPLC method respectively. ...
... The initial analysis was to determine the amount of neutral sugars and sialic acid forms using a phenol-sulfuric acid method and RP-HPLC method respectively. ...
Physiology of metabolic processes in the body. Composition of diet
... be developed. It has also been suggested as the ideal scale to rate proteins for their ability to meet human requirements. Similar to chemical score, it rates protein foods relative to a given reference protein. In this case, the AA profile used is that one determined to be ideal for children two to ...
... be developed. It has also been suggested as the ideal scale to rate proteins for their ability to meet human requirements. Similar to chemical score, it rates protein foods relative to a given reference protein. In this case, the AA profile used is that one determined to be ideal for children two to ...
Protein - people.vcu.edu
... Amino acids are colored to show different chemical properties. Note that the charged amino acids all lie not in the membrane but either inside or outside the red blood cell, as do most of the other hydrophilic amino acids. Many of the hydroxylated amino acids on the outside of the cell are also char ...
... Amino acids are colored to show different chemical properties. Note that the charged amino acids all lie not in the membrane but either inside or outside the red blood cell, as do most of the other hydrophilic amino acids. Many of the hydroxylated amino acids on the outside of the cell are also char ...
Protein Solubility as Quality Index for Processed Soybean (PDF
... processed SBM from under- or over-processed meals. During solvent processing of the soybean, lipids are removed and the meal is heated to eliminate the solvent (usually hexane) and to deactivate antinutritional factors such as trypsin inhibitors and lectin. Inadequate heating fails to completely des ...
... processed SBM from under- or over-processed meals. During solvent processing of the soybean, lipids are removed and the meal is heated to eliminate the solvent (usually hexane) and to deactivate antinutritional factors such as trypsin inhibitors and lectin. Inadequate heating fails to completely des ...
novel 4E-interacting protein in Leishmania is involved in stage
... The endogenous cap-binding proteins and their associated partners were isolated from wild-type L. amazonensis cells. Lysates were loaded on a m7GTP-Sepharose column, washed and eluted as described above. In all cases the eluted proteins were precipitated by TCA, resolved by SDS–PAGE (10–15%) and sub ...
... The endogenous cap-binding proteins and their associated partners were isolated from wild-type L. amazonensis cells. Lysates were loaded on a m7GTP-Sepharose column, washed and eluted as described above. In all cases the eluted proteins were precipitated by TCA, resolved by SDS–PAGE (10–15%) and sub ...
Role of the ubiquitinselective CDC48UFD1/NPL4 chaperone
... are toxic for yeast cells, the level of OLE1 is tightly regulated. Indeed, all known mechanisms that control UFA levels regulate OLE1 protein levels (McDonough et al., 1992; Choi et al., 1996; Gonzales and Martin, 1996). From these observations we hypothesized that OLE1 is in all likelihood a short- ...
... are toxic for yeast cells, the level of OLE1 is tightly regulated. Indeed, all known mechanisms that control UFA levels regulate OLE1 protein levels (McDonough et al., 1992; Choi et al., 1996; Gonzales and Martin, 1996). From these observations we hypothesized that OLE1 is in all likelihood a short- ...
QIAquick Gel Extraction Kit Protocol
... 2) Weigh the gel slice in a colorless tube. Add 3 volumes of Buffer QG to 1 volume of gel (100 mg ~ 100 µl). For >2% agarose gels, add 6 volumes of Buffer QG. The maximum amount of gel slice per QIAquick column is 400 mg; for gel slices >400 mg use more than one QIAquick column. 3) Incubate at 50°C ...
... 2) Weigh the gel slice in a colorless tube. Add 3 volumes of Buffer QG to 1 volume of gel (100 mg ~ 100 µl). For >2% agarose gels, add 6 volumes of Buffer QG. The maximum amount of gel slice per QIAquick column is 400 mg; for gel slices >400 mg use more than one QIAquick column. 3) Incubate at 50°C ...
Silver PA, Brent R, Ptashne M. DNA binding is not
... Certain proteins are found only in the cell nucleus. Following their synthesis in the cytoplasm, these proteins move into the nucleus in a way we do not understand. One possibility is that proteins diffuse into the nucleus through the nuclear pores and are retained there by binding to DNA or chromat ...
... Certain proteins are found only in the cell nucleus. Following their synthesis in the cytoplasm, these proteins move into the nucleus in a way we do not understand. One possibility is that proteins diffuse into the nucleus through the nuclear pores and are retained there by binding to DNA or chromat ...
c-MET - Siapec
... promising target for anticancer therapy VEGF has been demonstrated to be highly expressed in midgut ileal carcinoids whereas it is focally expressed in other gastroenteropancreatic neuroendocrine tumors, suggesting the possible use of this protein as a serum marker for ileal carcinoid ...
... promising target for anticancer therapy VEGF has been demonstrated to be highly expressed in midgut ileal carcinoids whereas it is focally expressed in other gastroenteropancreatic neuroendocrine tumors, suggesting the possible use of this protein as a serum marker for ileal carcinoid ...
Materials and methods (Supplement)
... The eluate containing GST-Tol2TP was added with PreScission protease (8 U for 5–10 mg of fusion protein; GE Healthcare Life Science) and dialyzed with PreScission cleavage buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.0, 1 mM EDTA, 1 mM DTT, 0.01% TritonX-100) at 4 ˚C for 12–16 hr to cleave the Tol2TP p ...
... The eluate containing GST-Tol2TP was added with PreScission protease (8 U for 5–10 mg of fusion protein; GE Healthcare Life Science) and dialyzed with PreScission cleavage buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.0, 1 mM EDTA, 1 mM DTT, 0.01% TritonX-100) at 4 ˚C for 12–16 hr to cleave the Tol2TP p ...
Long Noncoding RNAs Add Another Layer to Pre
... (ncRNAs) can be arbitrarily divided into short ncRNAs, typically !20–200 nt in length, and long ncRNAs ranging from !200 nt to 100 kb (Mercer et al., 2009). Although much evidence on the importance of the different types of short ncRNAs in the regulation of gene expression has accumulated, the funct ...
... (ncRNAs) can be arbitrarily divided into short ncRNAs, typically !20–200 nt in length, and long ncRNAs ranging from !200 nt to 100 kb (Mercer et al., 2009). Although much evidence on the importance of the different types of short ncRNAs in the regulation of gene expression has accumulated, the funct ...
LACTURE 2 PROTEINS
... polymerization of amino acids into peptides and proteins. Peptides are small consisting of few amino acids. The simplest peptide, a dipeptide, contains a single peptide bond formed by the condensation of the carboxyl group of one amino acid with the amino group of the second with the concomitant eli ...
... polymerization of amino acids into peptides and proteins. Peptides are small consisting of few amino acids. The simplest peptide, a dipeptide, contains a single peptide bond formed by the condensation of the carboxyl group of one amino acid with the amino group of the second with the concomitant eli ...
Theoretical studies of viral capsid proteins
... The pH dependence of the stability of picornavirus capsids [6,7•] has been examined using a dielectric continuum model [8]. The interfaces of the pentameric units of assembly were modeled from two unique protomers related by the twofold rotation of icosahedral symmetry. All titratable groups were co ...
... The pH dependence of the stability of picornavirus capsids [6,7•] has been examined using a dielectric continuum model [8]. The interfaces of the pentameric units of assembly were modeled from two unique protomers related by the twofold rotation of icosahedral symmetry. All titratable groups were co ...
File
... The ribosome receives instructions regarding what proteins to create and at the appropriate time. These instructions are originally found in DNA in the nucleus. A segment of DNA that codes for a particular protein is referred to as a gene. The specific sequence of nitrogen bases contained in the gen ...
... The ribosome receives instructions regarding what proteins to create and at the appropriate time. These instructions are originally found in DNA in the nucleus. A segment of DNA that codes for a particular protein is referred to as a gene. The specific sequence of nitrogen bases contained in the gen ...
Carbohydrates are split up into two groups
... regulate muscle contractions and helps blood clot normally with the help of vitamin K. Iron is the second mineral needed, its role is to make red blood cells and carry oxygen to cells and it is involved in energy production. Iron has two forms Haem and Non-Haem, Haem is involved in haemoglobin, whic ...
... regulate muscle contractions and helps blood clot normally with the help of vitamin K. Iron is the second mineral needed, its role is to make red blood cells and carry oxygen to cells and it is involved in energy production. Iron has two forms Haem and Non-Haem, Haem is involved in haemoglobin, whic ...
Use of infrared and visible light radiation as modulator of protein
... sufficient conditions for a specific electromagnetic radiation or absorption. In our previous research we have shown that such charge transfer through the protein backbone is possible through an exciton process [1,12]. The frequency range of this field depends on a charge velocity estimated to be 7. ...
... sufficient conditions for a specific electromagnetic radiation or absorption. In our previous research we have shown that such charge transfer through the protein backbone is possible through an exciton process [1,12]. The frequency range of this field depends on a charge velocity estimated to be 7. ...
Name_________________________________________
... i) Draw the side chains at amino acid positions 51, 129, 134, and 167. ii) Draw Minoxidil as shown above binding in the site. Be sure to consider the interactions between Minoxidil and the side chains when orienting Minoxidil within the binding site. ...
... i) Draw the side chains at amino acid positions 51, 129, 134, and 167. ii) Draw Minoxidil as shown above binding in the site. Be sure to consider the interactions between Minoxidil and the side chains when orienting Minoxidil within the binding site. ...
Translation - Protein Synthesis
... The next amino acid-tRNA attaches to the adjacent mRNA codon (leu in this case). ...
... The next amino acid-tRNA attaches to the adjacent mRNA codon (leu in this case). ...
lecturenotes.pdf
... channel in place within the membrane. (Amino acids 1 - 400 and 850 1200). They also form the walls of the ion channel. Note in the figure on the right that the transmembrane regions are actually placed so they form the walls of the ion channel when shown three dimensionally. • Two nucleotide binding ...
... channel in place within the membrane. (Amino acids 1 - 400 and 850 1200). They also form the walls of the ion channel. Note in the figure on the right that the transmembrane regions are actually placed so they form the walls of the ion channel when shown three dimensionally. • Two nucleotide binding ...
MASE1 and MASE2: Two Novel Integral Membrane Sensory Domains
... al., 2003]. An important feature of all those domains is their propensity to associate with more than one type of signal output domains (histidine kinases, adenylate cyclases, chemotaxis transducers), which made possible their recognition as conserved domains. In addition, these domains are often fo ...
... al., 2003]. An important feature of all those domains is their propensity to associate with more than one type of signal output domains (histidine kinases, adenylate cyclases, chemotaxis transducers), which made possible their recognition as conserved domains. In addition, these domains are often fo ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
... • The ultimate cellular location of proteins is often determined by specific, relatively short amino acid sequence within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
... • The ultimate cellular location of proteins is often determined by specific, relatively short amino acid sequence within the proteins themselves. These sequences can be responsible for proteins being secreted, imported into the nucleus or targeted to other organelles. ...
Amino Acid Metabolism
... • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N compounds, energy (also via gluco ...
... • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N compounds, energy (also via gluco ...
included data sources
... research institutes. Our unique configurable data loading platform enables us to ...
... research institutes. Our unique configurable data loading platform enables us to ...
1 Amino Acid Metabolism
... • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N compounds, energy (also via gluco ...
... • Metabolic pool AA has no storage form in mammals (as with other life forms) as free AA or as specialized storage form (such as glycogen for glucose, TG for FA) but a certain percentage of muscle & structural proteins are “expendable”. • AA are used for proteins, N compounds, energy (also via gluco ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.