Lecture 24
... Apart from the traditional methods for in vitro translation, new methods are being developed for cell-free protein expression. Many of these methods include production of proteins on a solid substrate – on a microarray. The following are some of the methods for in situ protein microarray production: ...
... Apart from the traditional methods for in vitro translation, new methods are being developed for cell-free protein expression. Many of these methods include production of proteins on a solid substrate – on a microarray. The following are some of the methods for in situ protein microarray production: ...
**** 1 - in-cosmetics Asia
... However, PTD (Protein Transduction Domain) technology enables effective transfer of these macromolecules possible. We developed 1:1 conjugated PTD and Growth factors such as PTD-EGF(Trade name : SP1-EGF) and PTD-FGF1(Trade name : SP1-FGF1). Our’s PTD technology is superior in terms of using native p ...
... However, PTD (Protein Transduction Domain) technology enables effective transfer of these macromolecules possible. We developed 1:1 conjugated PTD and Growth factors such as PTD-EGF(Trade name : SP1-EGF) and PTD-FGF1(Trade name : SP1-FGF1). Our’s PTD technology is superior in terms of using native p ...
Nanotech Meets Microbiology
... a top-down perspective. network might perform several different logical Going from molecules to the next higher levoperations. Hence, novel technologies and exels of biological organization (bottom-up) repertise will be needed to establish how such quires a quantitative understanding of how mocellul ...
... a top-down perspective. network might perform several different logical Going from molecules to the next higher levoperations. Hence, novel technologies and exels of biological organization (bottom-up) repertise will be needed to establish how such quires a quantitative understanding of how mocellul ...
amino acids
... • A multisubunit protein is also referred to as a multimer. • Multimeric proteins can have from two to hundreds of subunits. • A multimer with just a few subunits is often called an oligomer. • The repeating structural unit in such a multimeric protein, whether it is a single subunit or a group of s ...
... • A multisubunit protein is also referred to as a multimer. • Multimeric proteins can have from two to hundreds of subunits. • A multimer with just a few subunits is often called an oligomer. • The repeating structural unit in such a multimeric protein, whether it is a single subunit or a group of s ...
Stabilization of carbanions
... The reduction of NAD+ with 1,1-dideuteroethanol as the substrate gives 4R-[nicotinamide-4-2H2]NADH as product; the 2H is transferred specifically to the 4-pro-R site of NAD+.! ...
... The reduction of NAD+ with 1,1-dideuteroethanol as the substrate gives 4R-[nicotinamide-4-2H2]NADH as product; the 2H is transferred specifically to the 4-pro-R site of NAD+.! ...
Quiz 2 Review Sheet
... example to convince to me that mutations can be both positive and negative. Really well! Notice how it is #48.5…that means I squeezed it in…I wonder why…be able to tells this story on paper!!!!! 49. Describe in general terms what happens to a polypeptide (or any polymer for that matter) when you eat ...
... example to convince to me that mutations can be both positive and negative. Really well! Notice how it is #48.5…that means I squeezed it in…I wonder why…be able to tells this story on paper!!!!! 49. Describe in general terms what happens to a polypeptide (or any polymer for that matter) when you eat ...
Structure of Muscle Tissue
... Are organs of the muscular system There are more than 600 muscle in the animal body. Vary widely in shape, size and action. The structure of muscle will be showed in Figure ...
... Are organs of the muscular system There are more than 600 muscle in the animal body. Vary widely in shape, size and action. The structure of muscle will be showed in Figure ...
pGEX-5X-3 GST Expression Vector
... persons who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practice. Wear suitable protective clothing such as laboratory overalls, safety glasses and gloves. Care should be taken to avoid contact with skin or eyes. In the case of ...
... persons who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practice. Wear suitable protective clothing such as laboratory overalls, safety glasses and gloves. Care should be taken to avoid contact with skin or eyes. In the case of ...
Finding of a novel fungal immunomodulatory protein coding
... The search for transcripts (contigs) potentially related to immunomodulatory proteins allowed for the identification of 24 initial candidate sequences showing significant alignments to nonFIP immunomodulatory proteins (data not shown). A further BLAST search against GenBank non-redundant nucleotide ...
... The search for transcripts (contigs) potentially related to immunomodulatory proteins allowed for the identification of 24 initial candidate sequences showing significant alignments to nonFIP immunomodulatory proteins (data not shown). A further BLAST search against GenBank non-redundant nucleotide ...
Identification and Characterization of the Potato Leafroll Virus
... for the production of large quantities of sequence-specific antibodies. These are presently only produced in small amounts because of difficulties in obtaining large amounts of viral coat protein. Comparison of luteovirus coat proteins will also allow the prediction of common antigens, i.e. regions ...
... for the production of large quantities of sequence-specific antibodies. These are presently only produced in small amounts because of difficulties in obtaining large amounts of viral coat protein. Comparison of luteovirus coat proteins will also allow the prediction of common antigens, i.e. regions ...
IMGT/Collier-de-Perles: a two-dimensional visualization tool for
... The IMGT/Collier-de-Perles [14] tool was created by LIGM (Université Montpellier 2, CNRS) and is part of IMGTW, the international ImMunoGeneTics information systemW [15,16] (IMGTW, http://www.imgt.org), which is acknowledged as the global reference in immunogenetics and immunoinformatics. IMGT/Colli ...
... The IMGT/Collier-de-Perles [14] tool was created by LIGM (Université Montpellier 2, CNRS) and is part of IMGTW, the international ImMunoGeneTics information systemW [15,16] (IMGTW, http://www.imgt.org), which is acknowledged as the global reference in immunogenetics and immunoinformatics. IMGT/Colli ...
What are the major types of organic molecules?
... _____________ polypeptide chain, determined by _____________ structure combined with _____________ between _____________ groups (NOTE: book defines this in a confusing way, use my way) 4. quaternary structure (4) of a protein results from _____________ between _____________ or more _____________ po ...
... _____________ polypeptide chain, determined by _____________ structure combined with _____________ between _____________ groups (NOTE: book defines this in a confusing way, use my way) 4. quaternary structure (4) of a protein results from _____________ between _____________ or more _____________ po ...
Extended Project Description
... design of therapeutic inhibitors; many diseases are caused by dysregulation of cellular signalling processes. Most therapeutic inhibitors approved to now are for cancer therapies, but this focus is now broadening. Emerging targets of interest are relevant e.g. for neurodegenerative diseases, inflamm ...
... design of therapeutic inhibitors; many diseases are caused by dysregulation of cellular signalling processes. Most therapeutic inhibitors approved to now are for cancer therapies, but this focus is now broadening. Emerging targets of interest are relevant e.g. for neurodegenerative diseases, inflamm ...
Plant Nutrition: Root Transporters on the Move
... Plants take up nutrients and water from the soil and transport them to the leaves to support photosynthesis and plant growth. However, most soils around the world do not provide optimal conditions for plant colonization. Consequently, plants have evolved sophisticated mechanisms to adjust to deficien ...
... Plants take up nutrients and water from the soil and transport them to the leaves to support photosynthesis and plant growth. However, most soils around the world do not provide optimal conditions for plant colonization. Consequently, plants have evolved sophisticated mechanisms to adjust to deficien ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
... pin-bound peptides were held on rack, which had a 96-well format compatible with conventional ELISA plates. The peptide recognition was tested with sera (dilution 1:200) from DNA- or DHBc protein-immunized ducks or with sera from chronically infected animals. Direct ELISA was performed according to ...
... pin-bound peptides were held on rack, which had a 96-well format compatible with conventional ELISA plates. The peptide recognition was tested with sera (dilution 1:200) from DNA- or DHBc protein-immunized ducks or with sera from chronically infected animals. Direct ELISA was performed according to ...
Short transmembrane domains with high
... determinants of localization in mammalian cells, CHO-K1 cells were transfected with mCherry-tagged Sso1 or fusions of the Sso1 cytoplasmic domain to the TMDs described in Fig. 2 and their subcellular localization was established by colocalization with the plasma membrane marker K-Ras (Gomez and Dani ...
... determinants of localization in mammalian cells, CHO-K1 cells were transfected with mCherry-tagged Sso1 or fusions of the Sso1 cytoplasmic domain to the TMDs described in Fig. 2 and their subcellular localization was established by colocalization with the plasma membrane marker K-Ras (Gomez and Dani ...
Protein Requirements of Pregnant and Lactating Women
... the GWG was between 8 and 10 kg at term, for birth weights weight gain due to the fetus as well as to fluid gain and fat acranging from < 2500 to > 3000 g, in women whose mean height cretion. This is an important area of investigation. was 150 cm. However, a more recent assessment of GWG in eight co ...
... the GWG was between 8 and 10 kg at term, for birth weights weight gain due to the fetus as well as to fluid gain and fat acranging from < 2500 to > 3000 g, in women whose mean height cretion. This is an important area of investigation. was 150 cm. However, a more recent assessment of GWG in eight co ...
Microbiology Extra Credit asg
... clearly seen with the naked eye. Various types of microscopes and staining processes are used to observe and study microorganisms. 2.) Procaryotic cells lack a membranous nucleus; they have a nucleiod instead. Eukaryotic cells are typically bigger and have a more complex morphology than Procaryotic ...
... clearly seen with the naked eye. Various types of microscopes and staining processes are used to observe and study microorganisms. 2.) Procaryotic cells lack a membranous nucleus; they have a nucleiod instead. Eukaryotic cells are typically bigger and have a more complex morphology than Procaryotic ...
1 Biochemistry I: Proteins and Enzyme BIOC12H Summer 2015
... The objective of this course is to expand you understanding of the structure of proteins and the important role proteins play as enzymes, signaling molecules and structural molecules in the cell. Throughout the term we will discuss the importance of protein folding and post-translational modificatio ...
... The objective of this course is to expand you understanding of the structure of proteins and the important role proteins play as enzymes, signaling molecules and structural molecules in the cell. Throughout the term we will discuss the importance of protein folding and post-translational modificatio ...
DNA gel electrophoresis
... For 1% agarose add one gram agarose powder in 100 ml of the desired buffer. The mixture should be heated on a hot plate until boiling so the agarose can dissolve completely. Cool down the agarose mixture until 60 then pour off into a the casting tray. Place the comb and let the gel solidify. In the ...
... For 1% agarose add one gram agarose powder in 100 ml of the desired buffer. The mixture should be heated on a hot plate until boiling so the agarose can dissolve completely. Cool down the agarose mixture until 60 then pour off into a the casting tray. Place the comb and let the gel solidify. In the ...
Document
... built that way • The amino acids have very different chemical properties; they interact with each other after the protein is built • This causes the protein to start fold and adopting it’s functional structure • Proteins may fold in reaction to some ions, and several separate chains of peptides may ...
... built that way • The amino acids have very different chemical properties; they interact with each other after the protein is built • This causes the protein to start fold and adopting it’s functional structure • Proteins may fold in reaction to some ions, and several separate chains of peptides may ...
Allantoin transport protein, PucI, from Bacillus subtilis
... EcoRI and Pst I restriction sites at the 59 and 39 ends, respectively. The restriction-digested PCR product was ligated into plasmid pTTQ18 (Stark, 1987) immediately upstream from a hexahistidine tag coding sequence and the resultant pTTQ18-pucI(His6) construct was used to transform E. coli BL21(DE3 ...
... EcoRI and Pst I restriction sites at the 59 and 39 ends, respectively. The restriction-digested PCR product was ligated into plasmid pTTQ18 (Stark, 1987) immediately upstream from a hexahistidine tag coding sequence and the resultant pTTQ18-pucI(His6) construct was used to transform E. coli BL21(DE3 ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.