... environment, causing it to become a weaker acid because it would prefer to keep its proton. With Aspartic acid or Glutaminic acid the protonated form is uncharged. Therefore the deprotonated state would be favored with a positively charged environment making it a stronger acid. In contrast a negat ...
Protein Measurement with the Folin Phenol Reagent
... If the protein is present in an already very dilute solution (less than 25 y per ml.), 0.5 ml. may be mixed with 0.5 ml. of an exactly double strength Reagent C and otherwise treated as above. Insoluble Proteins, etc.-Many protein precipitates, e.g. tungstate preHowever, cipitates, will dissolve rea ...
... If the protein is present in an already very dilute solution (less than 25 y per ml.), 0.5 ml. may be mixed with 0.5 ml. of an exactly double strength Reagent C and otherwise treated as above. Insoluble Proteins, etc.-Many protein precipitates, e.g. tungstate preHowever, cipitates, will dissolve rea ...
10_Lecture
... The quaternary structure is held together through disulfide bridges between the polypeptide chains. The stem of the Y is similar in all antibodies and can bind to receptors on a variety of cells in the body. Antibodies bind antigens at the top of each arm of the Y. ...
... The quaternary structure is held together through disulfide bridges between the polypeptide chains. The stem of the Y is similar in all antibodies and can bind to receptors on a variety of cells in the body. Antibodies bind antigens at the top of each arm of the Y. ...
Ruboyianes - University of Arizona
... sodium chloride, 5 mM EDTA, 7.2 mM Na2HPO4, and 3.3 mM KH2PO4. Samples were mixed directly with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) running buffer and boiled. After SDS-PAGE, viral proteins were detected by Coomassie staining. Digitized images of protein gels were ge ...
... sodium chloride, 5 mM EDTA, 7.2 mM Na2HPO4, and 3.3 mM KH2PO4. Samples were mixed directly with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) running buffer and boiled. After SDS-PAGE, viral proteins were detected by Coomassie staining. Digitized images of protein gels were ge ...
Alpha Diagnostic Intl Inc., 6203 Woodlake Center Dr, San Antonio
... purified (>99%, mol wt ~43 kda) and used for control. For Western blot +ve control (Cat # ACTB19-C) is supplied in SDSPAGE sample buffer (reduced). Load 10 ul/lane of ACTB19-C for good visibility with antibody Cat # ACTB19-M or other antibodies. Store at –20oC in suitable size aliquots. SDS may crys ...
... purified (>99%, mol wt ~43 kda) and used for control. For Western blot +ve control (Cat # ACTB19-C) is supplied in SDSPAGE sample buffer (reduced). Load 10 ul/lane of ACTB19-C for good visibility with antibody Cat # ACTB19-M or other antibodies. Store at –20oC in suitable size aliquots. SDS may crys ...
Document
... Glutamate Receptor Interacting Protein: localized scaffold, mediation of trafficking of binding partners Protein Interacting w/ Protein Kinase C: adaptor that organizes subcellular location of membrane proteins Scaffold Protein Periplasmic Binding Protein: binds the Cterminal domains of transmembran ...
... Glutamate Receptor Interacting Protein: localized scaffold, mediation of trafficking of binding partners Protein Interacting w/ Protein Kinase C: adaptor that organizes subcellular location of membrane proteins Scaffold Protein Periplasmic Binding Protein: binds the Cterminal domains of transmembran ...
Lecture 2
... phosporylation/dephosphorylation can also directly change a proteins activity. However since such controls tend to be leaky, i.e. are the result of modification/demodification equilibria, their outcome depends on the initial abundance of the target protein. ...
... phosporylation/dephosphorylation can also directly change a proteins activity. However since such controls tend to be leaky, i.e. are the result of modification/demodification equilibria, their outcome depends on the initial abundance of the target protein. ...
Text - Enlighten - University of Glasgow
... In mammalian cells, several key enzymes of the polyamine pathway are subject to tight regulation (Persson, 2009). Ornithine decarboxylase (ODC), for example, is regulated by transcription, by translation and also by the stability of the protein itself, through binding of a regulatory protein known a ...
... In mammalian cells, several key enzymes of the polyamine pathway are subject to tight regulation (Persson, 2009). Ornithine decarboxylase (ODC), for example, is regulated by transcription, by translation and also by the stability of the protein itself, through binding of a regulatory protein known a ...
Abstract - WSU Horticulture
... calmodulin-binding assay to observe their recovery of affinity to calmodulin. We were able to identify a critical amino acid (T227) that interacts independently with these two phosphorylation sites. However, our results also suggest that there must be other interactor(s) that may be involved during ...
... calmodulin-binding assay to observe their recovery of affinity to calmodulin. We were able to identify a critical amino acid (T227) that interacts independently with these two phosphorylation sites. However, our results also suggest that there must be other interactor(s) that may be involved during ...
The Tryptophan Mutant in the Human Immunodeficiency Virus Type
... the proteins necessary for HIV survival and replication, is reverse transcribed into negatively oriented single stranded deoxyribonucleic acid which is then further copied/transcribed into double stranded DNA. The enzyme Reverse Transcriptase (RT) is the critical protein which catalyzes this process ...
... the proteins necessary for HIV survival and replication, is reverse transcribed into negatively oriented single stranded deoxyribonucleic acid which is then further copied/transcribed into double stranded DNA. The enzyme Reverse Transcriptase (RT) is the critical protein which catalyzes this process ...
Intracellular Protein Degradation
... mechanism of entry of cytosolic proteins into the lysosomal lumen, major problems have remained unsolved. Important among them were: (i) the varying half-lives, (ii) the energy requirement, and (iii) the distinct response of different populations of proteins to lysosomal inhibitors. Thus, according ...
... mechanism of entry of cytosolic proteins into the lysosomal lumen, major problems have remained unsolved. Important among them were: (i) the varying half-lives, (ii) the energy requirement, and (iii) the distinct response of different populations of proteins to lysosomal inhibitors. Thus, according ...
Chapter 3 - Slothnet
... specific molecules. Specificity is determined by: • Shape—there must be a general “fit” between the 3-D shapes of the protein and the other molecule. • Chemistry—R groups on the surface interact with other molecules via ionic, hydrophobic, or hydrogen bonds. ...
... specific molecules. Specificity is determined by: • Shape—there must be a general “fit” between the 3-D shapes of the protein and the other molecule. • Chemistry—R groups on the surface interact with other molecules via ionic, hydrophobic, or hydrogen bonds. ...
characterization of proteins from the cytoskeleton of giardia lamblia
... SDS gel filtration chromatography For peptide and amino acid analysis, a method was sought to separate pure samples of cytoskeleton proteins on a preparative scale. Experiments to solubilize tubulin and 30 X 103Mr protein from isolated cytoskeletons had resulted in low yields. When cytoskeletons wer ...
... SDS gel filtration chromatography For peptide and amino acid analysis, a method was sought to separate pure samples of cytoskeleton proteins on a preparative scale. Experiments to solubilize tubulin and 30 X 103Mr protein from isolated cytoskeletons had resulted in low yields. When cytoskeletons wer ...
The perichromatin region of the plant cell nucleus is the area with
... 2.5 lM EDTA, 5 mM NaF, 10 lM aprotinin, 10 lM leupeptin and 1 lM PMSF) and incubated on ice for 15 min. The crude protein extracts were centrifuged at 16,000g at 4 °C for 30 min. The pellet concentration was determined by the Bradford method (1976) using BSA as the standard. Aliquots of soluble prot ...
... 2.5 lM EDTA, 5 mM NaF, 10 lM aprotinin, 10 lM leupeptin and 1 lM PMSF) and incubated on ice for 15 min. The crude protein extracts were centrifuged at 16,000g at 4 °C for 30 min. The pellet concentration was determined by the Bradford method (1976) using BSA as the standard. Aliquots of soluble prot ...
Molecular characterization of the uncultivatable hemotropic
... Mycoplasma haemofelis was also found to have a very low percentage of intergenic regions with ORFs covering 94.9-94.3% of the genome, and ribosomal genes a further 0.9%. In contrast, the mean coding density of M. suis strains were 87.3-89.1%, whilst pneumoniae group mycoplasmas, to which the hemopla ...
... Mycoplasma haemofelis was also found to have a very low percentage of intergenic regions with ORFs covering 94.9-94.3% of the genome, and ribosomal genes a further 0.9%. In contrast, the mean coding density of M. suis strains were 87.3-89.1%, whilst pneumoniae group mycoplasmas, to which the hemopla ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
... The current review proposes updates on the estimation of the requirement of MP and essential AA for maintenance and milk. Current Estimations of Requirements Proteins This presentation will only deal with requirements for maintenance and lactation, considering mature cows as non-gestating and not ch ...
... The current review proposes updates on the estimation of the requirement of MP and essential AA for maintenance and milk. Current Estimations of Requirements Proteins This presentation will only deal with requirements for maintenance and lactation, considering mature cows as non-gestating and not ch ...
Aerobic Metabolism ii: electron transport chain
... In Complex IV (cytochrome c oxidase) sometimes called cytochrome A3, four electrons are removed from four molecules of cytochrome c (protein that loosely attached to the inner membrane, transfers electrons 1 at a time to cyt a)- and transferred to molecular oxygen (O2), producing two molecules of wa ...
... In Complex IV (cytochrome c oxidase) sometimes called cytochrome A3, four electrons are removed from four molecules of cytochrome c (protein that loosely attached to the inner membrane, transfers electrons 1 at a time to cyt a)- and transferred to molecular oxygen (O2), producing two molecules of wa ...
Prediction of mitochondrial proteins of malaria parasite
... malaria. In past, number of methods, including multi-subcellular localization and mitochondria specific, has been developed (Guda et al. 2004; Kumar et al. 2006). It is likely that these methods developed for eukaryotic proteins will also be valid for malaria parasite. Recently, it has been shown in ...
... malaria. In past, number of methods, including multi-subcellular localization and mitochondria specific, has been developed (Guda et al. 2004; Kumar et al. 2006). It is likely that these methods developed for eukaryotic proteins will also be valid for malaria parasite. Recently, it has been shown in ...
Characterization of the Plasmid-Encoded Arsenic Salts Resistance
... binding of the anions, which is required for hydrolysis of ATP [9]. The ArsB is an inner membrane protein and serves as the membrane anchor for the catalytic ArsA component [26], and mediates electrochemical energydependent arsenite efflux in the absence of the ArsA protein, while the ArsA-ArsB comp ...
... binding of the anions, which is required for hydrolysis of ATP [9]. The ArsB is an inner membrane protein and serves as the membrane anchor for the catalytic ArsA component [26], and mediates electrochemical energydependent arsenite efflux in the absence of the ArsA protein, while the ArsA-ArsB comp ...
Document
... The required signal sequence for a protein to enter the ER is 15– 30 N-terminal amino acids. As the signal sequence is produced by translation, it is bound by a signal recognition particle (SRP) composed of RNA and protein. The SRP suspends translation until the complex binds a docking protein on th ...
... The required signal sequence for a protein to enter the ER is 15– 30 N-terminal amino acids. As the signal sequence is produced by translation, it is bound by a signal recognition particle (SRP) composed of RNA and protein. The SRP suspends translation until the complex binds a docking protein on th ...
Diseases of the immune system
... of 88 combinatorial library samples stored in 96- well microtitre plates in 4–8 h [27]. Both FIA and DI analysis are capable of high throughput and automation. In FIA, a plug of sample is transported into the detector by a liquid stream, while DI analysis delivers a continuous sample flow to the det ...
... of 88 combinatorial library samples stored in 96- well microtitre plates in 4–8 h [27]. Both FIA and DI analysis are capable of high throughput and automation. In FIA, a plug of sample is transported into the detector by a liquid stream, while DI analysis delivers a continuous sample flow to the det ...
-Cell Calcium-Independent Group VIA Phospholipase A2 (iPLA2
... fusion protein was visualized by enhanced chemiluminescence after incubation with primary antibody, anti– green fluorescent protein (GFP) (IgG2␣, 0.0002 g/l), or anti-iPLA2 (0.0015 g/l) and then the appropriate secondary antibody. Insulin secretion. Cells were seeded in 24-well plates and allow ...
... fusion protein was visualized by enhanced chemiluminescence after incubation with primary antibody, anti– green fluorescent protein (GFP) (IgG2␣, 0.0002 g/l), or anti-iPLA2 (0.0015 g/l) and then the appropriate secondary antibody. Insulin secretion. Cells were seeded in 24-well plates and allow ...
Recombinant human insulin-11. Size-exclusion HPLC of
... pores, filled with the solvent, and are retained there for different time. Molecules, having a larger size in solution, either don't penetrate at all or penetrate only into part of the gel pores and are washed out of the column earlier, than small molecules, as the results of which separation accord ...
... pores, filled with the solvent, and are retained there for different time. Molecules, having a larger size in solution, either don't penetrate at all or penetrate only into part of the gel pores and are washed out of the column earlier, than small molecules, as the results of which separation accord ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.