Dm1-MMP, a Matrix Metalloproteinase from Drosophila with a
... SDS-PAGE. All assays were performed in the above described assay buffer for 16 h at 37 °C. The enzyme/substrate ratio (w/w) used in these experiments was 1/10. Substrate Gel Zymography—Casein zymography was done using a 13% SDS-polyacrylamide gel containing 1 mg/ml casein. Electrophoresis was perfor ...
... SDS-PAGE. All assays were performed in the above described assay buffer for 16 h at 37 °C. The enzyme/substrate ratio (w/w) used in these experiments was 1/10. Substrate Gel Zymography—Casein zymography was done using a 13% SDS-polyacrylamide gel containing 1 mg/ml casein. Electrophoresis was perfor ...
Document
... ◦ Cystic fibrosis is a hereditary disorder In some individuals, protein appears to have correct amino acid sequence but fails to fold ...
... ◦ Cystic fibrosis is a hereditary disorder In some individuals, protein appears to have correct amino acid sequence but fails to fold ...
Worked solutions to textbook questions 1 Chapter 12 Pathways to
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
Non-natural amino acid
... – isoleucyl-tRNA synthetase (IleRS) may load val onto tRNAile – there is an editing mechanism ...
... – isoleucyl-tRNA synthetase (IleRS) may load val onto tRNAile – there is an editing mechanism ...
Biological Polymers - McQuarrie General Chemistry
... Corey called the helix an α-helix (Figure T.3). The helical shape results from the formation of hydrogen bonds between peptide linkages in the peptide chain. Individually, these hydrogen bonds are relatively weak, but collectively they combine to bend the protein chain into the α-helix. This coiled, ...
... Corey called the helix an α-helix (Figure T.3). The helical shape results from the formation of hydrogen bonds between peptide linkages in the peptide chain. Individually, these hydrogen bonds are relatively weak, but collectively they combine to bend the protein chain into the α-helix. This coiled, ...
Drug discovery by single crystal X-ray structure analysis
... and common in both small molecule and macromolecule crystallography (Fig. 1). When a single crystal is irradiated with X-rays, they bounce off the electrons and interact to produce a set of diffraction spots, which researchers transform Fourier analysis to obtain the electron density inside the crys ...
... and common in both small molecule and macromolecule crystallography (Fig. 1). When a single crystal is irradiated with X-rays, they bounce off the electrons and interact to produce a set of diffraction spots, which researchers transform Fourier analysis to obtain the electron density inside the crys ...
Model Description Sheet
... interestingly, it can further process the 17-OH PREG by catalyzing the cleavage of the carbon 17, 20 bond as a next step, which is the first committed step in androgen biosynthesis. While the enzyme can similarly transform the 17-OH PROG, it does so with much lower efficiency, and it is this differe ...
... interestingly, it can further process the 17-OH PREG by catalyzing the cleavage of the carbon 17, 20 bond as a next step, which is the first committed step in androgen biosynthesis. While the enzyme can similarly transform the 17-OH PROG, it does so with much lower efficiency, and it is this differe ...
Direct Visualization by Cryo-EM of the Mycobacterial Capsular
... presence of detergent and agitation in order to prevent clumping ...
... presence of detergent and agitation in order to prevent clumping ...
Database Modeling in Bioinformatics
... The ideal sequence database for computational analyses and data-mining: • It must be complete with minimal redundancy • It must contain as much up-to-date information (annotation) as possible on each sequence • All the information items must be retrievable by computer programs in a consistent manne ...
... The ideal sequence database for computational analyses and data-mining: • It must be complete with minimal redundancy • It must contain as much up-to-date information (annotation) as possible on each sequence • All the information items must be retrievable by computer programs in a consistent manne ...
A RapidMiner framework for protein interaction extraction
... that is connected to a persistent storage (database) containing all instances. The actual operators use ExampleSets which are views over an ExampleTable that may represent partial information from this ExampleTable. An ExampleSet consists of Examples that usually correspond to feature vectors repres ...
... that is connected to a persistent storage (database) containing all instances. The actual operators use ExampleSets which are views over an ExampleTable that may represent partial information from this ExampleTable. An ExampleSet consists of Examples that usually correspond to feature vectors repres ...
339Kb Microsoft Powerpoint
... The ideal sequence database for computational analyses and data-mining: • It must be complete with minimal redundancy • It must contain as much up-to-date information (annotation) as possible on each sequence • All the information items must be retrievable by computer programs in a consistent manne ...
... The ideal sequence database for computational analyses and data-mining: • It must be complete with minimal redundancy • It must contain as much up-to-date information (annotation) as possible on each sequence • All the information items must be retrievable by computer programs in a consistent manne ...
Examination III Key
... 1 or 2 steps 1 or 2 steps Either “3 or 4 steps” or “more than 4 steps” are allowed as correct. This is because the cyclization reaction (Schiff base formation) is likely to be spontaneous and not catalyzed by an enzyme, hence only 4 enzymes are needed from α-KG, though it may take 5 reactions.) more ...
... 1 or 2 steps 1 or 2 steps Either “3 or 4 steps” or “more than 4 steps” are allowed as correct. This is because the cyclization reaction (Schiff base formation) is likely to be spontaneous and not catalyzed by an enzyme, hence only 4 enzymes are needed from α-KG, though it may take 5 reactions.) more ...
CURRICULUM VITAE
... Divergent synthesis are utilized from Resorcin[4]arene cavitands to develop novel cavitands with interesting binding and assembly properties in aqueous systems. Binding of Deep Cavity Cavitands to Specific Protein Targets, B.C. Gibb Deep cavity cavitands are shown to selectively and preferentially b ...
... Divergent synthesis are utilized from Resorcin[4]arene cavitands to develop novel cavitands with interesting binding and assembly properties in aqueous systems. Binding of Deep Cavity Cavitands to Specific Protein Targets, B.C. Gibb Deep cavity cavitands are shown to selectively and preferentially b ...
Part A: Amino Acids and Peptides
... 10. The chicken and the egg paradox speaks to the problem of how the first chicken got here. Explain how this parable relates to proteins. (Hint: see Model 2). Since proteins are needed to make other proteins, this creates a paradox… how was the first protein ...
... 10. The chicken and the egg paradox speaks to the problem of how the first chicken got here. Explain how this parable relates to proteins. (Hint: see Model 2). Since proteins are needed to make other proteins, this creates a paradox… how was the first protein ...
A New Signal Sequence for Recombinant Protein Secretion in
... into the P. pastoris (his−) strain. Transformation was carried out by electroporation of freshly prepared competent cells in 0.2 cm cuvettes. The pulses were delivered by a Gene Pulser (BioRad) at 1,500 V, 25 µF, and 200 Ω. The electroporated cells were allowed to recover for 1 h in 1 M Sorbitol at ...
... into the P. pastoris (his−) strain. Transformation was carried out by electroporation of freshly prepared competent cells in 0.2 cm cuvettes. The pulses were delivered by a Gene Pulser (BioRad) at 1,500 V, 25 µF, and 200 Ω. The electroporated cells were allowed to recover for 1 h in 1 M Sorbitol at ...
Engineering and Identifying Supercharged Proteins
... charged proteins such as þ36 GFP can penetrate mammalian cells with potencies much greater than that of cationic peptides or modestly cationic engineered proteins (McNaughton et al., 2009; Cronican et al. 2010; Cronican et al. 2011; Thompson et al., submitted). Live-cell fluorescence microscopy imag ...
... charged proteins such as þ36 GFP can penetrate mammalian cells with potencies much greater than that of cationic peptides or modestly cationic engineered proteins (McNaughton et al., 2009; Cronican et al. 2010; Cronican et al. 2011; Thompson et al., submitted). Live-cell fluorescence microscopy imag ...
Supporting Information
... with those observed in the overexpressing strains (Fig. S1,S2). However the levels of expression were reduced, which was consistent with the fact that cln3 upstream element 1 was driving gene expression in these constructs. ...
... with those observed in the overexpressing strains (Fig. S1,S2). However the levels of expression were reduced, which was consistent with the fact that cln3 upstream element 1 was driving gene expression in these constructs. ...
Hybridoma and Hybridomics
... Alcaligenes faecalis S6 was subcloned into the bacterial expression vector pQE 13 (Qiagen, Hilden, Germany) and the plasmid was introduced into E. coli JM 105. The enzyme was expressed as a fusion protein with N-terminal-6x His-tag under the control of a phage T5 Promotor/2 lac-Operator sequence and ...
... Alcaligenes faecalis S6 was subcloned into the bacterial expression vector pQE 13 (Qiagen, Hilden, Germany) and the plasmid was introduced into E. coli JM 105. The enzyme was expressed as a fusion protein with N-terminal-6x His-tag under the control of a phage T5 Promotor/2 lac-Operator sequence and ...
virus - Microbiology
... • However, since they can direct life processes they are often considered more than lifeless molecules ...
... • However, since they can direct life processes they are often considered more than lifeless molecules ...
Cardiovascular 19 – Homeostasis and Thrombosis
... o Haemophilia B factor IX deficiency (both are sex-linked) Symptoms of Haemophilia include haemoarthrosis (bleeding into joints). In blood coagulation disorders, the platelet plug is sufficient for small vessel injury whereas in larger vessels, the plug will fall apart. Superficial cuts do n ...
... o Haemophilia B factor IX deficiency (both are sex-linked) Symptoms of Haemophilia include haemoarthrosis (bleeding into joints). In blood coagulation disorders, the platelet plug is sufficient for small vessel injury whereas in larger vessels, the plug will fall apart. Superficial cuts do n ...
File
... » The chain of amino acids, called a polypeptide, will be released. It then undergoes some posttranslational processing in the golgi bodies and is transported via the endoplasmic reticulum (ER) » Ribosomes that are free, usually make proteins that are needed in the cell, while ribosomes that are bou ...
... » The chain of amino acids, called a polypeptide, will be released. It then undergoes some posttranslational processing in the golgi bodies and is transported via the endoplasmic reticulum (ER) » Ribosomes that are free, usually make proteins that are needed in the cell, while ribosomes that are bou ...
Microbially Derived Enzymes - Food Allergy Research and
... from the enzyme, concentration of the enzyme, and removal of impurities prior to final formulation with food-grade ingredients. Any potential residual fragments from the food allergen would be difficult to measure as there is no reliable assay. Commercial ELISAs are able to detect only intact protei ...
... from the enzyme, concentration of the enzyme, and removal of impurities prior to final formulation with food-grade ingredients. Any potential residual fragments from the food allergen would be difficult to measure as there is no reliable assay. Commercial ELISAs are able to detect only intact protei ...
Self-Interaction of the Herpes Simplex Virus Type 1
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
Leukaemia Section inv(8)(p11q13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Nuclear receptors are ligand-inductible trans-cription factors with three structural domains : an activation function AF-1, a DNA-binding domain and a second activation function AF-2 that is mediated by nuclear coactivators (NRCoAs) : TIF2 was recently shown to be one such mediators of AF-2 function ...
... Nuclear receptors are ligand-inductible trans-cription factors with three structural domains : an activation function AF-1, a DNA-binding domain and a second activation function AF-2 that is mediated by nuclear coactivators (NRCoAs) : TIF2 was recently shown to be one such mediators of AF-2 function ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.