Enzyme Assay for Glutathione S-Transferase Protocol
... Glutathione S Transferase (GST) is an enzyme involved in detoxification of a wide range of compounds and is involved in reducing free radical damage in red blood cells. The enzyme is easily purified by affinity chromatography and has been used as a fusion partner for many recombinant proteins. Ident ...
... Glutathione S Transferase (GST) is an enzyme involved in detoxification of a wide range of compounds and is involved in reducing free radical damage in red blood cells. The enzyme is easily purified by affinity chromatography and has been used as a fusion partner for many recombinant proteins. Ident ...
HOW TO USE BAD: A BRIEF PRESENTATION
... tension of the protein. These parameters are calculated from the respective amino acid contributions in mass, pKa, hydrophobicity (White & Wimley hydrophobicity scale), and surface tension. The surface tension of the amino acids has been estimated using SciPolymer software from SciVision. We also ca ...
... tension of the protein. These parameters are calculated from the respective amino acid contributions in mass, pKa, hydrophobicity (White & Wimley hydrophobicity scale), and surface tension. The surface tension of the amino acids has been estimated using SciPolymer software from SciVision. We also ca ...
Divalent Metal Ions in Plant Mitochondria and Their Role in
... could be traced to both the soluble and integral membrane protein fractions (Table II), which implies damage to mitochondrial soluble cuproproteins and also to cuprocomponents of the membrane-bound ETC. A 40% to 50% reduction of Fe and Mn content was also observed in the soluble protein fraction of ...
... could be traced to both the soluble and integral membrane protein fractions (Table II), which implies damage to mitochondrial soluble cuproproteins and also to cuprocomponents of the membrane-bound ETC. A 40% to 50% reduction of Fe and Mn content was also observed in the soluble protein fraction of ...
Effects of Enzyme Concentration, Temperature, pH
... Hydrolysis of protein was done by enzymatic hydrolysis using alcalase, an alkaline bacterial protease as a biocatalyst. Approximately 6 g of freeze dried PE were dissolved in 100 mL of phosphate buffers (0.1 M, pH7, 8 and 9) and heated at 95°C for 15 min. The hydrolysis was carried out by mixing 50 ...
... Hydrolysis of protein was done by enzymatic hydrolysis using alcalase, an alkaline bacterial protease as a biocatalyst. Approximately 6 g of freeze dried PE were dissolved in 100 mL of phosphate buffers (0.1 M, pH7, 8 and 9) and heated at 95°C for 15 min. The hydrolysis was carried out by mixing 50 ...
Determination of Blood Urea Nitrogen (BUN) Formation of Urea:
... The blood urea nitrogen (BUN) test is a measure of the amount of nitrogen in the blood in the form of urea, and a measurement of renal function. Urea is a byproduct from metabolism of proteins by the liver and is removed from the blood by the kidneys.The liver produces urea in the urea cycle as a wa ...
... The blood urea nitrogen (BUN) test is a measure of the amount of nitrogen in the blood in the form of urea, and a measurement of renal function. Urea is a byproduct from metabolism of proteins by the liver and is removed from the blood by the kidneys.The liver produces urea in the urea cycle as a wa ...
Materials by design: Merging proteins and music
... Please cite this article in press as: J.Y. Wong, et al., Nano Today (2012), http://dx.doi.org/10.1016/j.nantod.2012.09.001 ...
... Please cite this article in press as: J.Y. Wong, et al., Nano Today (2012), http://dx.doi.org/10.1016/j.nantod.2012.09.001 ...
Trimer Codon Mix 2 Antisense
... Different combination in percentage of the individual 20 amino acid mix codons can be ordered to be synthesized at the same codon position. Similarly there is no limit to the number of different sites that can be such modified. At Gene Link we term these sites as 'spiked'. Thus at a single codon pos ...
... Different combination in percentage of the individual 20 amino acid mix codons can be ordered to be synthesized at the same codon position. Similarly there is no limit to the number of different sites that can be such modified. At Gene Link we term these sites as 'spiked'. Thus at a single codon pos ...
CHAPTER 4: CELLULAR METABOLISM
... 3. Some enzymes are present in the cell’s cytoplasm, so those reactions occur in the cytosol, while other enzymes are present in the mitochondria of the cell, so those reactions occur in the mitochondria. 4. All organic molecules (carbohydrates, fats, and proteins) can be processed to release energy ...
... 3. Some enzymes are present in the cell’s cytoplasm, so those reactions occur in the cytosol, while other enzymes are present in the mitochondria of the cell, so those reactions occur in the mitochondria. 4. All organic molecules (carbohydrates, fats, and proteins) can be processed to release energy ...
Iron-Binding Activity of FutA1 Subunit of an ABC
... was centrifuged (12,000´g, 4°C, 10 min). The supernatant was transferred to a fresh tube, to which 1/50 volume of 50% slurry of Glutathione Sepharose 4B was added. The mixture was incubated at room temperature for 30 min with gentle agitation, then centrifuged and the supernatant was removed. After ...
... was centrifuged (12,000´g, 4°C, 10 min). The supernatant was transferred to a fresh tube, to which 1/50 volume of 50% slurry of Glutathione Sepharose 4B was added. The mixture was incubated at room temperature for 30 min with gentle agitation, then centrifuged and the supernatant was removed. After ...
A single amino acid substitution in the haemagglutinin
... decreased haemagglutinating activity, as compared with the wild-type parental strain. Sequencing of this recombinant virus showed a single mutation at amino acid position 192 of the HN protein (IleAMet). In the present study, we characterized that single amino acid substitution (I192M) in three stra ...
... decreased haemagglutinating activity, as compared with the wild-type parental strain. Sequencing of this recombinant virus showed a single mutation at amino acid position 192 of the HN protein (IleAMet). In the present study, we characterized that single amino acid substitution (I192M) in three stra ...
bi6103-20feb04 - NUS School of Computing
... • The first 3 and last 2 core states have to be traversed. But all other core states can be bypassed. • This models core regions of 5--25 residues Copyright 2003 limsoon wong ...
... • The first 3 and last 2 core states have to be traversed. But all other core states can be bypassed. • This models core regions of 5--25 residues Copyright 2003 limsoon wong ...
The Three-Dimensional Structure of the 15 Domain of the Human
... in stick representation (Cys 5 -Cys 40; Cys 18 -Cys 37; Cys 26 - Cys 58). Although evaluation of experimental data is still in progress a typical Kazal motif for dom15 can already be deduced, i.e. a small three-stranded β-sheet and a αhelix, located in a region of dom15 being consistent with the typ ...
... in stick representation (Cys 5 -Cys 40; Cys 18 -Cys 37; Cys 26 - Cys 58). Although evaluation of experimental data is still in progress a typical Kazal motif for dom15 can already be deduced, i.e. a small three-stranded β-sheet and a αhelix, located in a region of dom15 being consistent with the typ ...
Comparative Analysis of Two C-Terminal Kinesin Motor
... Protein extracts were prepared from tissue culture cells or mouse tissues by modification of published protocols [Bellve et al., 1977; Neely and Boekelheide, 1988]. Proteins were separated by polyacrylamide gel electrophoresis (PAGE) through 10% acrylamide gels, and equilibrated in and electrophoret ...
... Protein extracts were prepared from tissue culture cells or mouse tissues by modification of published protocols [Bellve et al., 1977; Neely and Boekelheide, 1988]. Proteins were separated by polyacrylamide gel electrophoresis (PAGE) through 10% acrylamide gels, and equilibrated in and electrophoret ...
ap® biology 2008 scoring guidelines - AP Central
... Question 1 Overview This question tested knowledge of protein form and function from the basic to the applied level. Students were asked to describe the bonds involved with protein structure and apply their knowledge of protein structure to specific functions, such as muscle contraction, cell signal ...
... Question 1 Overview This question tested knowledge of protein form and function from the basic to the applied level. Students were asked to describe the bonds involved with protein structure and apply their knowledge of protein structure to specific functions, such as muscle contraction, cell signal ...
BOULANT Steeve PhD
... Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. Boulant S, Vanbelle C, Ebel C, Penin F and Lavergne JP. J Virol.(IF 5.3) 2005. Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the viral RNA. ...
... Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. Boulant S, Vanbelle C, Ebel C, Penin F and Lavergne JP. J Virol.(IF 5.3) 2005. Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the viral RNA. ...
CHAPTER 22
... thousands are spotted to make a single array. Alternatively, short oligonucleotides can be directly synthesized on the surface of the slide. In this process, the DNA sequence at a given spot is produced by selectively controlling the growth of the oligonucleotide using narrow beams of light. In this ...
... thousands are spotted to make a single array. Alternatively, short oligonucleotides can be directly synthesized on the surface of the slide. In this process, the DNA sequence at a given spot is produced by selectively controlling the growth of the oligonucleotide using narrow beams of light. In this ...
Structure and Function of Membrane Proteins: Overview
... molecular se encontra bem determinada. ...
... molecular se encontra bem determinada. ...
the pdf - p53 WEB SITE
... 1 mM EDTA, 1.0% NP-40, 10 mM Tris (pH 8.0), 1.0% sodium deoxycolate and 16complete EDTA-free protease inhibitor cocktail (Roche). A total of 30 mg protein was loaded from each lysate and subjected to 10% SDS ± PAGE. Transfer of the proteins to PVDF membrane (Millipore) was performed by BioRad semi-d ...
... 1 mM EDTA, 1.0% NP-40, 10 mM Tris (pH 8.0), 1.0% sodium deoxycolate and 16complete EDTA-free protease inhibitor cocktail (Roche). A total of 30 mg protein was loaded from each lysate and subjected to 10% SDS ± PAGE. Transfer of the proteins to PVDF membrane (Millipore) was performed by BioRad semi-d ...
Eli Martinez poster
... reactive oxygen species (ROS). Manganese also acts as an enzyme cofactor for superoxide dismutase and other enzymes. In bacteria, manganese and Mn-SOD allow bacteria to withstand ROS created from internal and external metabolic processes. Pathogenic bacteria require manganese to remain virulent in e ...
... reactive oxygen species (ROS). Manganese also acts as an enzyme cofactor for superoxide dismutase and other enzymes. In bacteria, manganese and Mn-SOD allow bacteria to withstand ROS created from internal and external metabolic processes. Pathogenic bacteria require manganese to remain virulent in e ...
Intricacies and surprises of nuclear–mitochondrial co
... Here, the genetic instability of mt genomes comes into play, which predestines these organelles as a ‘playground’ for evolutionary variation. The mutation rate for mtDNA is at least 10 times higher than that of nuclear DNA, attributable to the poor fidelity of mtDNA polymerase γ , the ‘naked’ state ...
... Here, the genetic instability of mt genomes comes into play, which predestines these organelles as a ‘playground’ for evolutionary variation. The mutation rate for mtDNA is at least 10 times higher than that of nuclear DNA, attributable to the poor fidelity of mtDNA polymerase γ , the ‘naked’ state ...
- CSHL Institutional Repository
... Map1, Map2, and Ssb1 were expressed as His6-tagged versions in E. coli. For the quantification of ribosomes two proteins of the small ribosomal subunit (Asc1 and Rps9a) and two proteins of the large subunit (Rpl39 and Rpl17a) were expressed as His6-tagged versions in E. coli. For details see “Experi ...
... Map1, Map2, and Ssb1 were expressed as His6-tagged versions in E. coli. For the quantification of ribosomes two proteins of the small ribosomal subunit (Asc1 and Rps9a) and two proteins of the large subunit (Rpl39 and Rpl17a) were expressed as His6-tagged versions in E. coli. For details see “Experi ...
composition changes with age of plaque
... Outward diffusion of lactic acid produced from glycolysis of glucose Conversion of lactic acid into less ionized acetic and propionic acids The pH rise factor in saliva (sialin) which is a basic peptide containing Arg. It accelerate glucose uptake by salivary organisms, increase acid production & th ...
... Outward diffusion of lactic acid produced from glycolysis of glucose Conversion of lactic acid into less ionized acetic and propionic acids The pH rise factor in saliva (sialin) which is a basic peptide containing Arg. It accelerate glucose uptake by salivary organisms, increase acid production & th ...
Antigenic structure of foot-and-mouth disease virus
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
biomolecules
... could be a methyl (–CH3), or ethyl (–C2H 5) or higher number of –CH2 groups (1 carbon to 19 carbons). For example, palmitic acid has 16 carbons including carboxyl carbon. Arachidonic acid has 20 carbon atoms including the carboxyl carbon. Fatty acids could be saturated (without double bond) or unsat ...
... could be a methyl (–CH3), or ethyl (–C2H 5) or higher number of –CH2 groups (1 carbon to 19 carbons). For example, palmitic acid has 16 carbons including carboxyl carbon. Arachidonic acid has 20 carbon atoms including the carboxyl carbon. Fatty acids could be saturated (without double bond) or unsat ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.