Arabidopsis Contains Ancient Classes of Differentially Expressed
... promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained i ...
... promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained i ...
Molecular Docking
... Flexibility Through Soft Docking Methods The simple approach to tackle the protein flexibility problem is the 'soft docking' method It allows for slight penetrations between the receptor and the ligand molecules; this is a mathematical trick where the receptor and the ligand are held rigid and a ...
... Flexibility Through Soft Docking Methods The simple approach to tackle the protein flexibility problem is the 'soft docking' method It allows for slight penetrations between the receptor and the ligand molecules; this is a mathematical trick where the receptor and the ligand are held rigid and a ...
Production of recombinant EMA-1 protein and its
... respectively. The production of the recombinant histidine-tagged protein (His6-EMA1) was first evaluated in a small scale (50 mL) assay using 2YT medium containing 0.2% glucose and 30 μg.mL–1 kanamycin. The production of His6-EMA1 (34.2 kDa) was performed at 250 rev min–1 and both temperature of gro ...
... respectively. The production of the recombinant histidine-tagged protein (His6-EMA1) was first evaluated in a small scale (50 mL) assay using 2YT medium containing 0.2% glucose and 30 μg.mL–1 kanamycin. The production of His6-EMA1 (34.2 kDa) was performed at 250 rev min–1 and both temperature of gro ...
MCB303 - Salem University
... • Immunodifusion is often used as a tool in biochemical research to access the relatedness of protein from different sources. Immunediffusion by (Ring test) carried out in test tubes. • Method: Tubes containing Molten Agar into which different concentration of antiserum has been incorporated are pl ...
... • Immunodifusion is often used as a tool in biochemical research to access the relatedness of protein from different sources. Immunediffusion by (Ring test) carried out in test tubes. • Method: Tubes containing Molten Agar into which different concentration of antiserum has been incorporated are pl ...
NF2/Merlin and Neurofibromatosis 2
... documented cases of NF2 in order to confirm diagnosis. • Prenatal testing by amniocentesis and chorionic villi sampling is also available for those at risk. • The mean age of onset is in the early 20’s. • Those who present earlier with clinical symptoms tend to have more rapid, aggressive tumors wit ...
... documented cases of NF2 in order to confirm diagnosis. • Prenatal testing by amniocentesis and chorionic villi sampling is also available for those at risk. • The mean age of onset is in the early 20’s. • Those who present earlier with clinical symptoms tend to have more rapid, aggressive tumors wit ...
Differential display proteomic analysis of Picea meyeri pollen
... treated with 0.5% dimethyl sulfoxide (DMSO), representing the concentration used for 50 nM LATB dilution. Both control and treatment groups were observed at each time point to distinguish between responses to actin disruption and normal developmental changes in protein accumulation. The proteins wer ...
... treated with 0.5% dimethyl sulfoxide (DMSO), representing the concentration used for 50 nM LATB dilution. Both control and treatment groups were observed at each time point to distinguish between responses to actin disruption and normal developmental changes in protein accumulation. The proteins wer ...
A minimal gene set for cellular life derived by comparison of
... program (19). A modified version of SEG was used to predict nonglobular domains in proteins (19). Distinguishing Orthologs from Paralogs. Orthologs are genes related by vertical descent and are responsible for the same function in different organisms, in contrast to paralogs, which are homologs rela ...
... program (19). A modified version of SEG was used to predict nonglobular domains in proteins (19). Distinguishing Orthologs from Paralogs. Orthologs are genes related by vertical descent and are responsible for the same function in different organisms, in contrast to paralogs, which are homologs rela ...
Development and Validation of a Generic
... Methylation is a ubiquitous covalent modification used to control the function of diverse biomolecules including hormones, neurotransmitters, xenobiotics, proteins, nucleic acids and lipids. Histone methyltransferases (HMTs) are currently of high interest as drug targets because of their role in epi ...
... Methylation is a ubiquitous covalent modification used to control the function of diverse biomolecules including hormones, neurotransmitters, xenobiotics, proteins, nucleic acids and lipids. Histone methyltransferases (HMTs) are currently of high interest as drug targets because of their role in epi ...
Membrane Penetration of Cytosolic Phospholipase A2 Is Necessary
... of phospholipids (12, 13) and is generally thought to play a crucial role in maintaining cellular arachidonic acid levels (14). cPLA2 is therefore an attractive target for developing specific inhibitors that can be used as a novel antiinflammatory drugs. cPLA2 binds to membranes in the presence of m ...
... of phospholipids (12, 13) and is generally thought to play a crucial role in maintaining cellular arachidonic acid levels (14). cPLA2 is therefore an attractive target for developing specific inhibitors that can be used as a novel antiinflammatory drugs. cPLA2 binds to membranes in the presence of m ...
Pyruvate dehydrogenase complex
... of enzymes. Basic principles of metabolism. Common pathways of proteins, carbohydrates and lipids transformation. ...
... of enzymes. Basic principles of metabolism. Common pathways of proteins, carbohydrates and lipids transformation. ...
C urrent and prospective applications of metal ion–protein
... factor of the specificity reached in some IMAC based purification protocols. These chelating substances are attached on the sorbent surface via spacers (linkage groups) which can vary in length and composition. The final structure formed after the metal ion is chelated by the chelating group must al ...
... factor of the specificity reached in some IMAC based purification protocols. These chelating substances are attached on the sorbent surface via spacers (linkage groups) which can vary in length and composition. The final structure formed after the metal ion is chelated by the chelating group must al ...
Data-driven docking for the study of biomolecular complexes
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
Localization of Protein-Protein lnteractions between Subunits of
... A second phytochrome-responsivesystem would also be measuring total Pfr levels at higher light levels. This model provides a possible explanation for the extraordinary fluence sensitivity of this photoreceptor and emphasizes the importance of its dimeric structure in attaining this sensitivity. Also ...
... A second phytochrome-responsivesystem would also be measuring total Pfr levels at higher light levels. This model provides a possible explanation for the extraordinary fluence sensitivity of this photoreceptor and emphasizes the importance of its dimeric structure in attaining this sensitivity. Also ...
Localization of Protein-Protein lnteractions between Subunits of
... A second phytochrome-responsivesystem would also be measuring total Pfr levels at higher light levels. This model provides a possible explanation for the extraordinary fluence sensitivity of this photoreceptor and emphasizes the importance of its dimeric structure in attaining this sensitivity. Also ...
... A second phytochrome-responsivesystem would also be measuring total Pfr levels at higher light levels. This model provides a possible explanation for the extraordinary fluence sensitivity of this photoreceptor and emphasizes the importance of its dimeric structure in attaining this sensitivity. Also ...
clear liquid diet for endoscopy
... A clear liquid diet consists of clear liquids that are easily digested and leave no undigested residue in your intestinal tract. This is why a clear liquid diet must be followed before an Endoscopy procedure. Not following the diet correctly can affect the prep results and the ability to view your i ...
... A clear liquid diet consists of clear liquids that are easily digested and leave no undigested residue in your intestinal tract. This is why a clear liquid diet must be followed before an Endoscopy procedure. Not following the diet correctly can affect the prep results and the ability to view your i ...
Lecture 1 Amino acids and the Peptide Bond-I
... The groupings of the amino acids have been done in different ways – the major groups are hydrophobic and hydrophilic. Aliphatic and aromatic amino acids exist as do sulfur containing amino acids. There also polar amino acids that may be charged or uncharged. The acidic and basic amino acids remain c ...
... The groupings of the amino acids have been done in different ways – the major groups are hydrophobic and hydrophilic. Aliphatic and aromatic amino acids exist as do sulfur containing amino acids. There also polar amino acids that may be charged or uncharged. The acidic and basic amino acids remain c ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from porcine epididymal fluid (10). These procedures are not practical for studying a substantial number of mutant proteins, so we de ...
... and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from porcine epididymal fluid (10). These procedures are not practical for studying a substantial number of mutant proteins, so we de ...
Effect of Six Decades of Selective Breeding on
... Beckman 6300 Amino Acid Analyzer equipped with a postcolumn ninhydrin reaction detection system (Beckman Instruments, Fullerton, CA). Southern Hybridization. Total genomic DNA was isolated from leaf tissues of the genotypes listed in Table 1 using the CTAB method (17). Eight micrograms of genomic DN ...
... Beckman 6300 Amino Acid Analyzer equipped with a postcolumn ninhydrin reaction detection system (Beckman Instruments, Fullerton, CA). Southern Hybridization. Total genomic DNA was isolated from leaf tissues of the genotypes listed in Table 1 using the CTAB method (17). Eight micrograms of genomic DN ...
Ch. 3 Presentation
... 3.13 A protein’s shape depends on four levels of structure Protein secondary structure results from coiling or folding of the polypeptide. – Coiling results in a helical structure called an alpha helix. – A certain kind of folding leads to a structure called a pleated sheet, which dominates some ...
... 3.13 A protein’s shape depends on four levels of structure Protein secondary structure results from coiling or folding of the polypeptide. – Coiling results in a helical structure called an alpha helix. – A certain kind of folding leads to a structure called a pleated sheet, which dominates some ...
Mechanisms of the Spectral Shifts for Retinitis Pigmentosa Mutants
... the involved sites and its interaction with the protein environment in the dark state from mutants of human rhodopsin. We also analyze possible mechanisms on how these mutations can be related to energy balancing, photoisomerization and some potential side or harmful reactions. Structural and stabil ...
... the involved sites and its interaction with the protein environment in the dark state from mutants of human rhodopsin. We also analyze possible mechanisms on how these mutations can be related to energy balancing, photoisomerization and some potential side or harmful reactions. Structural and stabil ...
A MICROFLUIDIC CHIP COMBINING DNA EXTRACTION AND
... Figure 3: Real-time amplification plot for the S. aureus nuc gene. 10 µL of MSSA cell lysate was used as the sample for each well, while the presence of primers and probes was varied between wells. CONCLUSION Multiplexed PCR is usually done with all primer sets combined in a single reaction. It is ...
... Figure 3: Real-time amplification plot for the S. aureus nuc gene. 10 µL of MSSA cell lysate was used as the sample for each well, while the presence of primers and probes was varied between wells. CONCLUSION Multiplexed PCR is usually done with all primer sets combined in a single reaction. It is ...
Chemical Composition and Antimicrobial Activity of Royal Jelly
... any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into RJ [30]. The Jelleines are very short peptides, presenting hydrophobic sequences; these peptides do not present any similarity with any other known antimicrobial ...
... any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into RJ [30]. The Jelleines are very short peptides, presenting hydrophobic sequences; these peptides do not present any similarity with any other known antimicrobial ...
How flexible is α-actinin`s rod domain?
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
Enzymes - Land of Mayo
... brought about by other chemicals can activate or inhibit depending of shape change without the control agent the enzyme’s shape might not fit the substrate ...
... brought about by other chemicals can activate or inhibit depending of shape change without the control agent the enzyme’s shape might not fit the substrate ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.