Chem*3560 Lecture 11: Regulation by proteolytic cleavage
... 15 and Ile 16, at a location which is structurally equivalent to Lys6 and Ile7 in trypsinogen. The newly exposed N-terminal Ile is similarly positioned to make an ion pair with Asp 194, and the mechanism of activation is the same as for trypsin. The immediate product is called π -chymotrypsin and is ...
... 15 and Ile 16, at a location which is structurally equivalent to Lys6 and Ile7 in trypsinogen. The newly exposed N-terminal Ile is similarly positioned to make an ion pair with Asp 194, and the mechanism of activation is the same as for trypsin. The immediate product is called π -chymotrypsin and is ...
Communication, Homeostasis
... decarboxylase, Lactate dehydrogenase ethanol dehydrogenase Define the term respiratory substrate. An organic substance that can be used for respiration Carbohydrates is the primary respiratory substrate Protein would be regarded as the respiratory substrate that is only used if no others are a ...
... decarboxylase, Lactate dehydrogenase ethanol dehydrogenase Define the term respiratory substrate. An organic substance that can be used for respiration Carbohydrates is the primary respiratory substrate Protein would be regarded as the respiratory substrate that is only used if no others are a ...
Types and effects of protein variations. Vihinen
... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... SDS-PAGE and Western blot analysis. Cell samples were lysed in PBS containing 1% Triton X-100 and protease inhibitor cocktail (Roche, Almere, Netherlands) and sonified. Cell debris was removed by centrifugation, and the protein concentration was determined using a BCA Protein Assay kit. For sBARF1, ...
... SDS-PAGE and Western blot analysis. Cell samples were lysed in PBS containing 1% Triton X-100 and protease inhibitor cocktail (Roche, Almere, Netherlands) and sonified. Cell debris was removed by centrifugation, and the protein concentration was determined using a BCA Protein Assay kit. For sBARF1, ...
Predicting the Secondary Structure of Globular Proteins Using
... in the protein sequence is likely to produce significantly better results for non-homologous proteins. The performance of our method of homologous proteins is much better than for non-homologous proteins, but is not as good as simply assuming that homologous sequences have identical structures. ...
... in the protein sequence is likely to produce significantly better results for non-homologous proteins. The performance of our method of homologous proteins is much better than for non-homologous proteins, but is not as good as simply assuming that homologous sequences have identical structures. ...
msb145697-sup-0001-Supp_Info
... To estimate the fraction of total protein mass covered by mass spectrometry, we rely on two pieces of information: 1) the highly non-uniform distribution of individual protein mass as given by the method of spectral counting (shown above); and 2) the absolute protein quantitation results from the 2D ...
... To estimate the fraction of total protein mass covered by mass spectrometry, we rely on two pieces of information: 1) the highly non-uniform distribution of individual protein mass as given by the method of spectral counting (shown above); and 2) the absolute protein quantitation results from the 2D ...
Structural Basis of Perturbed pKa Values of Catalytic Groups in
... nucleophiles, electrophiles, general-base catalysts, or generalacid catalysts. The key to their adapted role or function lies in their state of protonation, and the state of protonation is determined, at first approximation, by the intrinsic pK a of the given group. Table 1 lists the intrinsic pK a ...
... nucleophiles, electrophiles, general-base catalysts, or generalacid catalysts. The key to their adapted role or function lies in their state of protonation, and the state of protonation is determined, at first approximation, by the intrinsic pK a of the given group. Table 1 lists the intrinsic pK a ...
Chemical of Life
... 18. Be able to describe the secondary, tertiary and quaternary levels protein structures and, in general terms, how they occur. 19. Be able to explain what protein motifs and domains are. 20. Be able to explain what physically happens to a protein when it becomes denatured, what can cause this and ...
... 18. Be able to describe the secondary, tertiary and quaternary levels protein structures and, in general terms, how they occur. 19. Be able to explain what protein motifs and domains are. 20. Be able to explain what physically happens to a protein when it becomes denatured, what can cause this and ...
The Behavior of Proteins: Enzymes, Mechanisms
... This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of t ...
... This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of t ...
Low Protein Diet - Hamilton Health Sciences
... Note: Another source of certain amino acids is the sweetener "aspartame". Depending on the condition your child has, aspartame may need to be avoided. Ask your dietitian if your child needs to avoid this chemical. ...
... Note: Another source of certain amino acids is the sweetener "aspartame". Depending on the condition your child has, aspartame may need to be avoided. Ask your dietitian if your child needs to avoid this chemical. ...
ref. #28 of the TIBS article
... of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: the phage M13 coat protein We have shown that the N ...
... of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: the phage M13 coat protein We have shown that the N ...
Amino Acids and Proteins Amino Acid Compound
... Tyrosine has an a-CO2H pKa of 2.20, and a-NH3+ pKa of 9.11, and a sidechain pKa of 10.07 - sketch a titration curve and give the structure of the major form or forms present at all equivalence and half-equivalence points. ...
... Tyrosine has an a-CO2H pKa of 2.20, and a-NH3+ pKa of 9.11, and a sidechain pKa of 10.07 - sketch a titration curve and give the structure of the major form or forms present at all equivalence and half-equivalence points. ...
Planta
... was used for recombination with plant and yeast expression vectors through LR reactions. In fusions of fluorescent proteins to the C terminus of VvCTr1, the stop codon was omitted in the reverse primer. For subcellular localization studies, the entry clone carrying VvCTr1 was recombined into pH7FWG2 ...
... was used for recombination with plant and yeast expression vectors through LR reactions. In fusions of fluorescent proteins to the C terminus of VvCTr1, the stop codon was omitted in the reverse primer. For subcellular localization studies, the entry clone carrying VvCTr1 was recombined into pH7FWG2 ...
9/12
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
MS PowerPoint - Catalysis Eprints database
... • Ionic interactions between an enzyme-bound metal and a substrate help orient the substrate for reaction or stabilize charged reaction transition states. • Metals also mediate oxidation-reduction reactions by reversible changes in the metal ion’s oxidation state. • For example – in hemoglobin Fe in ...
... • Ionic interactions between an enzyme-bound metal and a substrate help orient the substrate for reaction or stabilize charged reaction transition states. • Metals also mediate oxidation-reduction reactions by reversible changes in the metal ion’s oxidation state. • For example – in hemoglobin Fe in ...
Chapter 3 The Same 20 Amino Acids Serve as Building Blocks for
... 5.3.1 Amino acids will be eluted out in the order of their binding affinity (strongly bound ones being eluted out later). 5.3.2 This way of separating amino acids (or other charged biomolecules) is called ion-exchange chromatography. 5.3.3 Chromatography is a method of separating substances by allow ...
... 5.3.1 Amino acids will be eluted out in the order of their binding affinity (strongly bound ones being eluted out later). 5.3.2 This way of separating amino acids (or other charged biomolecules) is called ion-exchange chromatography. 5.3.3 Chromatography is a method of separating substances by allow ...
Aligning protein sequences by hand
... heat labile and denatures at temperatures higher than 52 oC. What will you do? Introducing some mutations may make the protein more stable. Simple, but which of the 298 amino acids should be mutated? The only thing we know is that we should not mutate in or near the active site because that would al ...
... heat labile and denatures at temperatures higher than 52 oC. What will you do? Introducing some mutations may make the protein more stable. Simple, but which of the 298 amino acids should be mutated? The only thing we know is that we should not mutate in or near the active site because that would al ...
ppt
... -We have considered discrete “levels” of structure within a hierarchical model. -In constructing the hierarchy is it natural to start from the smallest scale and to build up. -We have borrowed from proteins in labeling the hierarchical levels primary, secondary, tertiary and quaternary. -The hierarc ...
... -We have considered discrete “levels” of structure within a hierarchical model. -In constructing the hierarchy is it natural to start from the smallest scale and to build up. -We have borrowed from proteins in labeling the hierarchical levels primary, secondary, tertiary and quaternary. -The hierarc ...
5 Production of Microbial Biomass - Wiley-VCH
... knowledge of the metabolic pathways involved, determination of the nutrient requirements of the microorganism and the physicochemical conditions required for optimum growth. Furthermore, development and changes in culture equipment thanks to progress in process engineering enable optimization of the ...
... knowledge of the metabolic pathways involved, determination of the nutrient requirements of the microorganism and the physicochemical conditions required for optimum growth. Furthermore, development and changes in culture equipment thanks to progress in process engineering enable optimization of the ...
M6PRs are found in a subset of PC12 cell ISGs
... identified which are clathrin-coated and contain M6PRs (Klumperman et al., 1998). It is not yet known whether these pathways overlap, ie if the clathrin-coated vesicles also contain C-peptide. Finally, it has not yet been determined if the M6PR positive, clathrin coated membranes and vesicles also c ...
... identified which are clathrin-coated and contain M6PRs (Klumperman et al., 1998). It is not yet known whether these pathways overlap, ie if the clathrin-coated vesicles also contain C-peptide. Finally, it has not yet been determined if the M6PR positive, clathrin coated membranes and vesicles also c ...
Protein Utilization in Bacteria
... Bacteria can use a wide variety of substrates present in the environment as sources of nutrition; these include simple or complex carbohydrates, lipids, nucleic acids and proteins. The oxidation of proteins for energy production requires more complex enzymatic processes than the oxidation of carbohy ...
... Bacteria can use a wide variety of substrates present in the environment as sources of nutrition; these include simple or complex carbohydrates, lipids, nucleic acids and proteins. The oxidation of proteins for energy production requires more complex enzymatic processes than the oxidation of carbohy ...
Full Text
... 3MATRIX and 3MOTIF are designed for interoperability with other bioinformatics resources on the Internet. Linking to these tools is straightforward, with detailed instructions available on the main web pages. Examples of this are additions to the eMOTIF-SEARCH component of the eMOTIF software suite ...
... 3MATRIX and 3MOTIF are designed for interoperability with other bioinformatics resources on the Internet. Linking to these tools is straightforward, with detailed instructions available on the main web pages. Examples of this are additions to the eMOTIF-SEARCH component of the eMOTIF software suite ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.