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lecture3
lecture3

... regulatory submit has a pseudosubstrate sequence that mimics the target sequence but lacks OH-bearing side chain at the right place. For, e.g. the cAMP-binding regulatory sub-units of protein Kinase A possess the pseudo substrate sequence that binds to the active site of protein Kinase A catalytic s ...
Practice Exam III answers
Practice Exam III answers

Enzyme Activity
Enzyme Activity

... Inhibitors are chemicals that reduce the rate of enzymic reactions. The are usually specific and they work at low concentrations. They block the enzyme but they do not usually destroy it. ...
Protein Digestion and Absorption
Protein Digestion and Absorption

Transferase-catalyses transfer of a group from one molecule to
Transferase-catalyses transfer of a group from one molecule to

L5 Metabolism Part2 Fa08
L5 Metabolism Part2 Fa08

... – Binds to enzyme away from active site – Changes conformation of enzyme/active site ...
Enzymes
Enzymes

Practice Exam 1 Answers
Practice Exam 1 Answers

... 12. This amino acid residue often prevents the formation of an α helix because its side chain contains a unique ring structure that restricts bond rotations. A. Aspartate B. Serine C. Proline D. Histidine E. Glycine 13. Which one of these characteristics is NOT true for the  helix? A) There are 3. ...
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... How Enzymes Work: • Enzymes are made up of long chains of amino acids • Enzymes attach to substrates in order to work • Most enzymes have globular shapes with active sites – Where the substrate binds ...
Water - UCLA Chemistry and Biochemistry
Water - UCLA Chemistry and Biochemistry

... • Preferential binding of transition state: binding interactions between the enzyme and TS are maximized; they are greater than those in the enzyme-substrate or enzyme-product complexes • General acid and general base catalysis: functional groups of the enzyme donate &/or accept protons • Covalent c ...
Biomolecules review with answers
Biomolecules review with answers

... proteins. Protein shapes fall into 4 categories: Primary is straight, Secondary is twisted and folded into sheets and helices, Tertiary is a complex inter linking for chains, and Quaternary which is the noncovalent binding of multiple tertiary complexes. 38. How do living things use steroids? Estrog ...
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Structural Insights into Catalysis and Inhibition of O

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Word Notes - Eric Hamber Secondary

... metabolic rate (rate of the chem. reactions in the cell) in all the cells in your body. The more thyroxin present the greater the metabolic rate. This will increase sugar and oxygen consumption and also creates more body heat. ...
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I can - Net Start Class

... A. Enzymes are composed of amino acid chains. B. Enzymes form a temporary association with a reactant. C. Enzymes are destroyed when they are used. D. Enzymes are specific because of their shape. 2. Enzymes only work with specific substrates because each substrate— A. has a specific activation site ...
Mechanisms of Enzyme Regulation • Substrate concentration
Mechanisms of Enzyme Regulation • Substrate concentration

... Product inhibition. If the product accumulates, it can inhibit some enzymes. This form of control limits the rate of formation of the product when the product is underused. Besides you can remember that Enzymes do not affect equilibrium constants. It means that increasing product concentration cause ...
enzymes - BEHS Science
enzymes - BEHS Science

Practice Exam1
Practice Exam1

... 12. This amino acid residue often prevents the formation of an α helix because its side chain contains a unique ring structure that restricts bond rotations. A. Aspartate B. Serine C. Proline D. Histidine E. Glycine 13. Which one of these characteristics is NOT true for the  helix? A) There are 3. ...
Biology Passage 2 - HCC Learning Web
Biology Passage 2 - HCC Learning Web

Glycoside hydrolases: Catalytic base
Glycoside hydrolases: Catalytic base

Chapter 2 - Biochemistry
Chapter 2 - Biochemistry

... • There are a large number of different types of proteins: – The number, kind and sequence of amino acids lead to this large variety ...
Faculty of Science, IUG
Faculty of Science, IUG

CHNOPS Lab
CHNOPS Lab

... place. The code, in DNA or mRNA, specifies the order in which the amino acids are joined together to form a polypeptide. As the code carried by mRNA is “read” on a ribosome, the amino acids are added to the growing polypeptide chain (protein) . The process by which the information from DNA is transf ...
Topic 3 – The Chemistry of Life
Topic 3 – The Chemistry of Life

CHEM523 Final Exam
CHEM523 Final Exam

... b) Indicate the two protons which would be lost at pH 12. c) Circle and label two hydrogen bond donors and two acceptors on your structure. d) Write the 1-letter code for each of the three amino acids below the structure. e) Indicate with an arrow which peptide bond might be found in the cis conform ...
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Catalytic triad



A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.
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