Synthesis of Heme
... Schiff base linkage at each of 2 ALA binding sites. a yeast PBG Synthase crystallized with ALA substrate, having at its active site an intermediate resembling PBG in Schiff base linkage to one lysine side-chain. ...
... Schiff base linkage at each of 2 ALA binding sites. a yeast PBG Synthase crystallized with ALA substrate, having at its active site an intermediate resembling PBG in Schiff base linkage to one lysine side-chain. ...
Allelic Amino Acid Substitutions Affect the
... to p2o9 substitution (4), but additional amino acid replacements have been identified for the I (intermediate) variant ...
... to p2o9 substitution (4), but additional amino acid replacements have been identified for the I (intermediate) variant ...
Microsoft Word
... The sequence specific cleavage of the peptide bond has been an issue of interest in protein in biotechnology. We thoroughly studied the hydrolysis assisted by Ni(II) ions in R N(Ser/Thr)-Xaa-His-Zaa-RC peptide sequences. First, we found that hydrolysis occurred when Xaa was Ala or His, Zaa was Lys, ...
... The sequence specific cleavage of the peptide bond has been an issue of interest in protein in biotechnology. We thoroughly studied the hydrolysis assisted by Ni(II) ions in R N(Ser/Thr)-Xaa-His-Zaa-RC peptide sequences. First, we found that hydrolysis occurred when Xaa was Ala or His, Zaa was Lys, ...
A STUDY OF THE ROLES OF SELECTED ARGININE AND
... carboxypeptidase B-like enzyme that catalyzes removal of basic (arginine or lysine) residues from the carboxy termini of selected peptides or proteins [4, 10]. In the process of fibrin clot dissolution, the fibrin mesh stimulates its lysis by plasmin (Figure 1-3). Fibrin not only is the substrate fo ...
... carboxypeptidase B-like enzyme that catalyzes removal of basic (arginine or lysine) residues from the carboxy termini of selected peptides or proteins [4, 10]. In the process of fibrin clot dissolution, the fibrin mesh stimulates its lysis by plasmin (Figure 1-3). Fibrin not only is the substrate fo ...
Midazolam Oxidation by Cytochrome P450 3A4 and Active
... CYP3A4 (Gorski et al., 1994; Kuehl et al., 2001). The oxidation of MDZ by CYP3A4 has been the focus of many in vitro investigations (Kronbach et al., 1989; Gorski et al., 1994; Ghosal et al., 1996; Maenpaa et al., 1998; Hosea et al., 2000; Wang et al., 2000). These studies have found very different ...
... CYP3A4 (Gorski et al., 1994; Kuehl et al., 2001). The oxidation of MDZ by CYP3A4 has been the focus of many in vitro investigations (Kronbach et al., 1989; Gorski et al., 1994; Ghosal et al., 1996; Maenpaa et al., 1998; Hosea et al., 2000; Wang et al., 2000). These studies have found very different ...
STUDIES ON THE CHLORAMPHENICOL HALOGENASE CmlS
... Figure 1-8 Initial reaction scheme for a flavin dependent halogenase............................................. 9 Figure 1-9 Mechanism of chlorination of tryptophan by PrnA and RebH. ................................... 11 Figure 2-1 Putative biosynthetic pathway for the antibiotic chloramphenico ...
... Figure 1-8 Initial reaction scheme for a flavin dependent halogenase............................................. 9 Figure 1-9 Mechanism of chlorination of tryptophan by PrnA and RebH. ................................... 11 Figure 2-1 Putative biosynthetic pathway for the antibiotic chloramphenico ...
Methods and strategies of peptide ligation
... occupying NT positions can be considered as NTamine-nucleophiles. Since not all amino acids contain a side-chain nucleophile and since NT-amine-nucleophiles have different reactivity toward the complementary CT-acyl-electrophiles, they provide a mechanism to distinguish one NT-amine from another. Th ...
... occupying NT positions can be considered as NTamine-nucleophiles. Since not all amino acids contain a side-chain nucleophile and since NT-amine-nucleophiles have different reactivity toward the complementary CT-acyl-electrophiles, they provide a mechanism to distinguish one NT-amine from another. Th ...
ESTUDIO DE LOS MECANISMOS DE INHIBICIÓN DE LA ACTIVIDAD CARNITINA PALMITOILTRANSFERASA 1
... Residues Conserved in Malonyl-CoA Inhibited Versus Noninhibited Carnitine-Choline Acyltransferases—An exhaustive analysis of the presence of residues shared by all the malonylCoA-regulated enzymes of the carnitine-choline acyltransferase family versus the malonyl-CoA nonregulated members of the same ...
... Residues Conserved in Malonyl-CoA Inhibited Versus Noninhibited Carnitine-Choline Acyltransferases—An exhaustive analysis of the presence of residues shared by all the malonylCoA-regulated enzymes of the carnitine-choline acyltransferase family versus the malonyl-CoA nonregulated members of the same ...
Biomimetic Reactions Catalyzed by Cyclodextrins and
... 13).198 In 18, an imidazole carries a benzoate group in a position to imitate the function of the aspartate ion in the catalytic triad characteristic of serine proteases such as chymotrypsin (Figure 14).199-203 It was claimed that compound 18 acted to hydrolyze a bound substrate using the imidazole ...
... 13).198 In 18, an imidazole carries a benzoate group in a position to imitate the function of the aspartate ion in the catalytic triad characteristic of serine proteases such as chymotrypsin (Figure 14).199-203 It was claimed that compound 18 acted to hydrolyze a bound substrate using the imidazole ...
C H A P
... (Grippa et al., 1999; Higaki, 2003; Gupta et al., 2004). Moreover, inhibition studies on lipases could contribute to better understand the mechanism of action of these enzymes, favoring the design of evolved lipases with improved biochemical properties and more suitable for biotechnological applicat ...
... (Grippa et al., 1999; Higaki, 2003; Gupta et al., 2004). Moreover, inhibition studies on lipases could contribute to better understand the mechanism of action of these enzymes, favoring the design of evolved lipases with improved biochemical properties and more suitable for biotechnological applicat ...
Functional characterization of the Mycobacterium
... Eukaryotic-like Ser/Thr protein kinases (STPKs) are present in many bacterial species, where they control various physiological and virulence processes by enabling microbial adaptation to specific environmental signals. PknJ is the only member of the 11 STPKs identified in Mycobacterium tuberculosis ...
... Eukaryotic-like Ser/Thr protein kinases (STPKs) are present in many bacterial species, where they control various physiological and virulence processes by enabling microbial adaptation to specific environmental signals. PknJ is the only member of the 11 STPKs identified in Mycobacterium tuberculosis ...
Sequence and Structure Classification of Kinases
... the fold and biochemistry within this group. The availability of thousands of kinase sequences and hundreds of kinase structures coupled with a wealth of biochemical data make kinases an ideal group of enzymes for such structural/functional classification and analysis. We have analyzed over 17,000 k ...
... the fold and biochemistry within this group. The availability of thousands of kinase sequences and hundreds of kinase structures coupled with a wealth of biochemical data make kinases an ideal group of enzymes for such structural/functional classification and analysis. We have analyzed over 17,000 k ...
The Role of N- and C-terminal Amino Acids to
... thesis. Without his time and advice, this project would not have been possible. I would now like to thank my parents for their support throughout my seven years of postsecondary education. You were always there for me when I needed additional encouragement and I am forever grateful. Thank you to my ...
... thesis. Without his time and advice, this project would not have been possible. I would now like to thank my parents for their support throughout my seven years of postsecondary education. You were always there for me when I needed additional encouragement and I am forever grateful. Thank you to my ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
Catalytic triad
A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.