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... transduction system used by the bacterium to respond to and regulate the uptake of the siderophore ferrioxamine (Llamas et al., 2006). Siderophores are high-affinity ironchelating compounds that are produced and secreted by bacteria to solubilize the minute amounts of bioavailable iron present in th ...
Research Article Identification and Characterization of Cell Wall
Research Article Identification and Characterization of Cell Wall

... to various aspects of cell surface associated ecophysiology. However, there are few experimental data available for the cell wall of dinoflagellates compared with other organisms due to the lack of the whole genome. So far, only a limited number of cell wall proteins (CWPs) and enzymes have been ide ...
The diguanylate cyclase YddV controls production of
The diguanylate cyclase YddV controls production of

... However, for several genes encoding putative DGCs, their functional role in the production of adhesion factors has not been fully determined. For instance, yddV, arguably the most highly expressed DGC-encoding gene in E. coli (Sommerfeldt et al., 2009), can activate transcription of the csgBA operon ...
Supplementary information
Supplementary information

... was purified using anti-His antibody-CNBr-activated Sepharose 4B (60 mg; Amersham Biosciences AB, Uppsala, Sweden). Approximately 5 µg (5 µL) of purified protein in binding buffer (Beyotime Institute of Biotechnology, Shanghai, China) was incubated in 100 fmol digoxigenin (Dig)-labeled FoxO binding ...
Surveying Protein Structure and Function Using Bis
Surveying Protein Structure and Function Using Bis

... fluorescence when coordinated to simple dithiol ligands such as ethanedithiol (EDT), but became increasingly fluorescent when bound by the four thiols present within the linear sequence CCRECC. When this sequence was encoded at the N- or C-terminus of a protein expressed in mammalian cells, treatmen ...
Identification and Characterization of cvHsp
Identification and Characterization of cvHsp

... adjustments for the separation of the largest from the next-to-largest intensity. In addition, the algorithm estimates its own reliability in order to distinguish high confidence calculations from more ambiguous ones. The algorithm has been implemented to routinely analyze large data bases of gene a ...
Protein Secretion in Plants: from the trans
Protein Secretion in Plants: from the trans

... acting as molecular scaffolds and regulators of membrane composition. As many as 57 rabs have been identified in Arabidopsis, nearly matching the number of rabs in mammals and by far exceeding those of yeast, Drosophila or Caenorhabditis elegans (44). Rab functions are conserved across eukaryotes, su ...
Thermodynamic prediction of protein neutrality
Thermodynamic prediction of protein neutrality

... substitutions is of fundamental importance in understanding natural protein evolution, developing protein engineering strategies, and understanding the basis of genetic diseases. Computational and experimental studies have demonstrated that both protein stability and structure affect a protein’s tol ...
H_355605_Nenova, Bulgaria.fm
H_355605_Nenova, Bulgaria.fm

... They are indispensable because they cannot be synthesized in the human organism and therefore must be supplied in the diet by plants and micro organisms. Other twelve amino acids are not essential and the human organism is able to produce them when needed. The nutrition value of sunflower protein is ...
Protein Data Condensation for Effective Quaternary Structure
Protein Data Condensation for Effective Quaternary Structure

... The main contribution of the work [5] is the exploitation of a pseudo amino acid composition to express a protein in terms of a set of discrete numbers, which is more powerful than the standard amino acid composition. In fact, it is able to take into account a considerable amount of sequence-order e ...
Notes - Part 2.
Notes - Part 2.

... The most extensive -helices in proteins occur not singly but in combination: 2 or 3 helices lie parallel and in register and fold together to form a superhelix or coiled coil. These occur in the myosin of muscle and other eukaryotic cells; in the intermediate filaments of the cytoskeleton (a multi ...
Acidaminococcus fermentans type strain (VR4T)
Acidaminococcus fermentans type strain (VR4T)

... Different from most organisms for which the type strain genomes have so far been described in the GEBA series, A. fermentans strain VR4T is biochemically well described. The strain has been intensively studied for many years. Here, we describe the genomic location of the genes for the biochemically ...
Proteomics_12-8
Proteomics_12-8

... Nesvizhskii (2010) J. Proteomics, 73:20922123. ...
Identification of the factors that interact with NCBP, an 80 kDa
Identification of the factors that interact with NCBP, an 80 kDa

... cap structure by itself (3). It is interesting that the cap recognition mechanisms of the two cellular compartments are very different. In this respect it is noteworthy that some tryptophan residues in eIF-4E, conserved among species, are important for cap binding activity (3), whereas no similar se ...
Gene Section CLTCL1 (clathrin heavy polypeptide-like 1) Atlas of Genetics and Cytogenetics
Gene Section CLTCL1 (clathrin heavy polypeptide-like 1) Atlas of Genetics and Cytogenetics

... Online updated version : http://AtlasGeneticsOncology.org/Genes/CLTCL1ID361.html DOI: 10.4267/2042/37779 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2001 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Structural alignment - Structural Biology Labs
Structural alignment - Structural Biology Labs

... H (homologous superfamily) Sequence identity >= 35%, 60% of larger structure equivalent to smaller SSAP score >= 80.0 and sequence identity >= 20% 60% of larger structure equivalent to smaller SSAP score >= 80.0, 60% of larger structure equivalent to smaller and domains which have related functions ...
J-Domain Protein CDJ2 and HSP70B Are a Plastidic Chaperone
J-Domain Protein CDJ2 and HSP70B Are a Plastidic Chaperone

... shown to increase the cell’s ability to cope with photoinhibition, possibly by either protecting photosystem II from irreversible photodamage or by stabilizing photodamaged photosystem II for a coordinated exchange of damaged D1 protein for de novo-synthesized D1 (Schroda et al., 1999, 2001a). Other ...
The Complete Post-Exercise Muscle Hydration Recovery
The Complete Post-Exercise Muscle Hydration Recovery

... An exciting area in the molecular biochemistry of protein synthesis are the role of Heat Shock Proteins (HSP). HSP are molecular chaperones that aid in the transport of proteins throughout the cell’s various compartments to support protein synthesis. HSP or chaperones also protect the newly synthesi ...
Cell cycle control by ubiquitylation
Cell cycle control by ubiquitylation

... are periodic1. This is the result of a constant synthetic rate coupled with a defined window in the cycle of specific proteolysis, which is executed by the ubiquitinproteasome ...
Enzyme Properties - Illinois Institute of Technology
Enzyme Properties - Illinois Institute of Technology

... This happens for several reasons Active protein needs to bind cofactors, ions, carbohydrates, and other species Active protein might be dangerous at the ribosome, so it’s created in inactive form and activated elsewhere ...
Proteins
Proteins

... • Unlike other caseins, κ-caseins are glycoproteins, and they have only 2 phosphate group. • Hence, they are stable in the presence of calcium ions, and they play an important role in protecting other caseins from precipitation and make casein more soluble forming casein micelles. • Neither the α n ...
BIOLOGICAL MACROMOLECULES
BIOLOGICAL MACROMOLECULES

... The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particu ...
Replacing Traditional SDS-PAGE and Bradford Techniques
Replacing Traditional SDS-PAGE and Bradford Techniques

Molecular cloning, expression, and bioactivity of dove B lymphocyte
Molecular cloning, expression, and bioactivity of dove B lymphocyte

... B cell activating factor (BAFF) belonging to the tumor necrosis factor (TNF) family is a novel member of the tumor necrosis factor ligand family and plays an important role in B lymphocyte maturation and survival. cDNA of dove B lymphocyte stimulator (doBAFF) was amplified from total RNA of dove sple ...
Trypsin, Active Trypsin, Active
Trypsin, Active Trypsin, Active

... Step 3. Trypsin digestion can be stopped by freezing or by lowering the pH of the reaction below pH 4 by adding formic, acetic, or trifluoroacetic acid (a) Trypsin will regain activity above pH 4 (b) Digested samples can be stored at -20°C ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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