Amino Acid Metabolism
... considerable expenditure of energy, possibly more than is required for protein synthesis • Protein turnover contributes at least 20% to resting energy expenditure (basal metabolic rate) The Lysosomal –Autophagic System • In general, extracellular, membrane-associated, and longlived intracellular pro ...
... considerable expenditure of energy, possibly more than is required for protein synthesis • Protein turnover contributes at least 20% to resting energy expenditure (basal metabolic rate) The Lysosomal –Autophagic System • In general, extracellular, membrane-associated, and longlived intracellular pro ...
Lecture 5
... B. Activity 2. slow (indirect) - metabotropic - or modulates opening of channels in response to voltage changes (neuromodulation) ...
... B. Activity 2. slow (indirect) - metabotropic - or modulates opening of channels in response to voltage changes (neuromodulation) ...
GRA Proteins of Toxoplasma gondii: Maintenance of Host
... of an expression cDNA library with the mAb led to the isolation of the longest one being 1,030 bp [27], which consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an O-glycosylation [28]. The p ...
... of an expression cDNA library with the mAb led to the isolation of the longest one being 1,030 bp [27], which consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an O-glycosylation [28]. The p ...
Physiological role of insulin
... – Increased lipoprotein metabolism • Lipoprotein lipase – Increased free fatty acids release ...
... – Increased lipoprotein metabolism • Lipoprotein lipase – Increased free fatty acids release ...
Lecture: 27 Fatty acid and triacyl glycerol biosynthesis Biosynthesis
... i. Synthesis takes place in the cytosol, in contrast with degradation or oxidation, which occurs in the mitochondrial matrix. ii. Intermediates in fatty acid synthesis are covalently linked to the sulfhydryl group of an acyl carrier protein (ACP) whereas intermediates in fatty acid breakdown are bon ...
... i. Synthesis takes place in the cytosol, in contrast with degradation or oxidation, which occurs in the mitochondrial matrix. ii. Intermediates in fatty acid synthesis are covalently linked to the sulfhydryl group of an acyl carrier protein (ACP) whereas intermediates in fatty acid breakdown are bon ...
Physiological adaptations of Saccharomyces cerevisiae evolved for
... yeast genome [40], this was the only transposon associated protein detected in our samples, and the biological relevance of this finding is therefore hard to judge. We are planning to sequence the genomes of the JBA-mut and JBA-wt strains which might shed some light on this issue. The second most re ...
... yeast genome [40], this was the only transposon associated protein detected in our samples, and the biological relevance of this finding is therefore hard to judge. We are planning to sequence the genomes of the JBA-mut and JBA-wt strains which might shed some light on this issue. The second most re ...
ENZYME STUDIES ON CHROMOSOMES [ 40 ]
... seven days, then stained by the Feulgen method. No digestion of chromosomes was observed to have taken place. It will be interesting to see whether glands of young larvae may not behave differently. Also, we should expect to find an effective protease in the pupa. Protaminase. Well (1935) has descri ...
... seven days, then stained by the Feulgen method. No digestion of chromosomes was observed to have taken place. It will be interesting to see whether glands of young larvae may not behave differently. Also, we should expect to find an effective protease in the pupa. Protaminase. Well (1935) has descri ...
Gelatinization of Starch
... Similar to amino acids, proteins can be either positively or negatively charged due to the terminal amine -NH2 and carboxyl (-COOH) groups. ...
... Similar to amino acids, proteins can be either positively or negatively charged due to the terminal amine -NH2 and carboxyl (-COOH) groups. ...
Investigation of Iron-Sulfur Protein Maturation in Eukaryotes
... approx 20 assembly components known to date, many are encoded by essential genes, including several components of mitochondria. This indicates that the process is indispensable for life. In fact, the maturation of cellular Fe-S proteins is so far the only mitochondrial function that is essential for ...
... approx 20 assembly components known to date, many are encoded by essential genes, including several components of mitochondria. This indicates that the process is indispensable for life. In fact, the maturation of cellular Fe-S proteins is so far the only mitochondrial function that is essential for ...
Metabolism of Plasma Membrane Lipids in
... reported in Mycobacterium, Streptomyces, Rhodococcus and Nocardia [48-52]. Interestingly, corynebacteria seem to lack the capacity to synthesize TAG, indicating that some lineages of Actinobacteria have eliminated this capacity at some point in their evolution. Recent evidence suggests that M. tuber ...
... reported in Mycobacterium, Streptomyces, Rhodococcus and Nocardia [48-52]. Interestingly, corynebacteria seem to lack the capacity to synthesize TAG, indicating that some lineages of Actinobacteria have eliminated this capacity at some point in their evolution. Recent evidence suggests that M. tuber ...
emboj200897-sup
... itself as the substrate (Soulat et al., 2007). While the PTK serves as both the kinase and the substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for ...
... itself as the substrate (Soulat et al., 2007). While the PTK serves as both the kinase and the substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level of the samples. Supplementary Figure 3 shows a similar starting phosphorylation level for ...
PURIFICATION OF TAP TAGGED YEAST PROTEINS Annika Väntänen
... long-chain fatty acids. These mitochondrially synthesised long-chain fatty acids, for example 3-hydroxymyristoyl-ACP, are possibly used for protein acylation which is one type of lipid modifications of proteins. The best characterised physiological function of the pathway is to provide the octanoyl ...
... long-chain fatty acids. These mitochondrially synthesised long-chain fatty acids, for example 3-hydroxymyristoyl-ACP, are possibly used for protein acylation which is one type of lipid modifications of proteins. The best characterised physiological function of the pathway is to provide the octanoyl ...
2. - Blue Ridge Institute for Medical Research
... system. Its approach reflects very much the way in which immunological research operates today: cells dealt with at the molecular level, the context of which encompasses their surface receptors for antigen, cytokines, other cells and so on; how their genes are regulated; and how they communicate wit ...
... system. Its approach reflects very much the way in which immunological research operates today: cells dealt with at the molecular level, the context of which encompasses their surface receptors for antigen, cytokines, other cells and so on; how their genes are regulated; and how they communicate wit ...
Defining the molecular basis for the first potent and selective
... To address this need, we identified compounds from the patent literature with reported activity at FFA2 that we hypothesized were likely to be orthosteric agonists, based primarily on the presence, as in the SCFAs, of a carboxylate moiety (16). We synthesized two compounds based on the structures de ...
... To address this need, we identified compounds from the patent literature with reported activity at FFA2 that we hypothesized were likely to be orthosteric agonists, based primarily on the presence, as in the SCFAs, of a carboxylate moiety (16). We synthesized two compounds based on the structures de ...
Growing Membranes, Sustaining Cells
... and connected by transporters to other pathways. Instead, some pathways operate across two or more compartments. In other words, some pathways are segmented into pieces operating in different compartments in eukaryotic cells, where membrane transporters become part of the pathway itself, as is well ...
... and connected by transporters to other pathways. Instead, some pathways operate across two or more compartments. In other words, some pathways are segmented into pieces operating in different compartments in eukaryotic cells, where membrane transporters become part of the pathway itself, as is well ...
Membrane Protein Expression in Cell
... Fig. 11.1. Modes and systems for the CF expression of MPs. (a) Expression modes for the CF production of MPs. In the precipitate mode (P-CF), no hydrophobic environments are provided, and the MPs precipitate after translation. In the detergent-based mode (D-CF), the synthesised MPs can stay soluble ...
... Fig. 11.1. Modes and systems for the CF expression of MPs. (a) Expression modes for the CF production of MPs. In the precipitate mode (P-CF), no hydrophobic environments are provided, and the MPs precipitate after translation. In the detergent-based mode (D-CF), the synthesised MPs can stay soluble ...
Multiple Choice Questions- Chemistry and Metabolism of nucleotides
... 19- A 7-year-old boy suffers from mental retardation and self mutilation and has an increased levels of serum uric acid. These symptoms are characteristic of Lesch Nyhan syndrome, which is due to defectivea) Salvage pathway for pyrimidine biosynthesis b) Denovo synthesis of pyrimidines c) Xanthine ...
... 19- A 7-year-old boy suffers from mental retardation and self mutilation and has an increased levels of serum uric acid. These symptoms are characteristic of Lesch Nyhan syndrome, which is due to defectivea) Salvage pathway for pyrimidine biosynthesis b) Denovo synthesis of pyrimidines c) Xanthine ...
(enzyme).
... Only the correctly sized key (substrate) fits into the key hole (active site) of the lock (enzyme). ...
... Only the correctly sized key (substrate) fits into the key hole (active site) of the lock (enzyme). ...
G protein
... Many G Proteins Use Inositol Trisphosphate and Diacylglycerol as Second Messengers • Inositol-1,4,5-trisphosphate (IP3) functions as a second messenger • It is generated from PIP2 when the enzyme phospholipase C is activated • It cleaves PIP2 into IP3 and diacylglycerol, both of which are second me ...
... Many G Proteins Use Inositol Trisphosphate and Diacylglycerol as Second Messengers • Inositol-1,4,5-trisphosphate (IP3) functions as a second messenger • It is generated from PIP2 when the enzyme phospholipase C is activated • It cleaves PIP2 into IP3 and diacylglycerol, both of which are second me ...
The nature of mycelial lipolytic enzymes in filamentous fungi
... organism. Since apparent phospholipase At activities of fungal preparations have been attributed to lipases [4,5] it was of interest to compare the relative lipase and phospholipase activities of the mycelial samples. Table 2 shows the variation in lipase and phospholipase activities of each organis ...
... organism. Since apparent phospholipase At activities of fungal preparations have been attributed to lipases [4,5] it was of interest to compare the relative lipase and phospholipase activities of the mycelial samples. Table 2 shows the variation in lipase and phospholipase activities of each organis ...
Sensing the fat: Fatty acid metabolism in the
... reported that changes in hypothalamic malonyl-CoA levels correlate with the effects of ICV C75 on the expression of NPY, AGRP, and POMC [15] (Fig. 2). However, it is not known how accumulation of malonyl-CoA is linked to changes in neuropeptide gene expression. Several possible mechanisms have been ...
... reported that changes in hypothalamic malonyl-CoA levels correlate with the effects of ICV C75 on the expression of NPY, AGRP, and POMC [15] (Fig. 2). However, it is not known how accumulation of malonyl-CoA is linked to changes in neuropeptide gene expression. Several possible mechanisms have been ...
NEURAL TUBE DEFECT ROLE OF FOLIC ACID
... • Folate provides one-carbon units for the de-novo synthesis of DNA bases (guanidine, adenine, and thymine). • Folate requirements increase dramatically during the periods of rapid growth of fetus. • Thus with folate deficiency, DNA synthesis is inhibited and cells are unable to manufacture enough D ...
... • Folate provides one-carbon units for the de-novo synthesis of DNA bases (guanidine, adenine, and thymine). • Folate requirements increase dramatically during the periods of rapid growth of fetus. • Thus with folate deficiency, DNA synthesis is inhibited and cells are unable to manufacture enough D ...
Fatty acids with
... addition of carnitine -secondary carnitine deficiency due to deficiency on β-oxidation acyl-carnitine in the urine Carnititne acyltransferase deficiency most common - CPT II gene mutation – partial loss of enzyme activity ...
... addition of carnitine -secondary carnitine deficiency due to deficiency on β-oxidation acyl-carnitine in the urine Carnititne acyltransferase deficiency most common - CPT II gene mutation – partial loss of enzyme activity ...
... Most enzymes are pH dependent for their activity. Usually they have a pH-optimum which is suited to the environment in which they are generally found. Reasons for this may be that the amino acids in the active site need to be in a certain state of ionization to be active, that the substrate has to ...
Deorphanization of GPRC6A: a promiscuous L-α
... analysis showed that the longest isoform of GPRC6A was expressed at the highest level in human tissues, and an orthologue of that form was also found in mice (Genbank accession #AY101365), . GPRC6A displays 45% amino acid sequence identity with the goldfish odorant receptor 5.24 (Speca et al., 1999) ...
... analysis showed that the longest isoform of GPRC6A was expressed at the highest level in human tissues, and an orthologue of that form was also found in mice (Genbank accession #AY101365), . GPRC6A displays 45% amino acid sequence identity with the goldfish odorant receptor 5.24 (Speca et al., 1999) ...
Lipid signaling
Lipid signaling, broadly defined, refers to any biological signaling event involving a lipid messenger that binds a protein target, such as a receptor, kinase or phosphatase, which in turn mediate the effects of these lipids on specific cellular responses. Lipid signaling is thought to be qualitatively different from other classical signaling paradigms (such as monoamine neurotransmission) because lipids can freely diffuse through membranes (see osmosis.) One consequence of this is that lipid messengers cannot be stored in vesicles prior to release and so are often biosynthesized ""on demand"" at their intended site of action. As such, many lipid signaling molecules cannot circulate freely in solution but, rather, exist bound to special carrier proteins in serum.