Design, Synthesis, and Antibacterial Properties of Dual
... moiramide B was solved in complex with carboxyltransferase. The structure shows that only the (S)-methyl pyrrolidinedione head group of moiramide B is used to inhibit the enzyme by taking advantage of the two oxyanion holes that the enzyme normally uses to stabilize enolate anions that form in the s ...
... moiramide B was solved in complex with carboxyltransferase. The structure shows that only the (S)-methyl pyrrolidinedione head group of moiramide B is used to inhibit the enzyme by taking advantage of the two oxyanion holes that the enzyme normally uses to stabilize enolate anions that form in the s ...
David Rittenberg - National Academy of Sciences
... of various compounds in the animal body. The metabolites containing 2H had properties essentially indistinguishable from their natural analogs by the methods commonly used. Nevertheless, the presence of the isotope made it possible to trace their metabolic fate. Thus, if a 2H-containing compound, B, ...
... of various compounds in the animal body. The metabolites containing 2H had properties essentially indistinguishable from their natural analogs by the methods commonly used. Nevertheless, the presence of the isotope made it possible to trace their metabolic fate. Thus, if a 2H-containing compound, B, ...
Extreme sweetness: protein glycosylation in archaea
... have revealed that in M. maripaludis, modified proteins are decorated with a tetrasaccharide that can be distinguished from its M. voltae counterpart through the use of N‑acetylgalactosamine instead of GlcNAc as the linking first sugar, by the presence of a 3‑acetamidino moiety on the third sugar su ...
... have revealed that in M. maripaludis, modified proteins are decorated with a tetrasaccharide that can be distinguished from its M. voltae counterpart through the use of N‑acetylgalactosamine instead of GlcNAc as the linking first sugar, by the presence of a 3‑acetamidino moiety on the third sugar su ...
The Amino-acid Sequence in the Phenylalanyl Chain of Insulin
... On hydrolysis of certain aromatic peptides a new spot was observed moving slightly faster than tyrosine in phenol and almost as fast as leucine in butanol-acetic acid. It gave a positive reaction with diazotized sulphanilic acid. In many cases pairs of peptides were found, one containing tyrosine an ...
... On hydrolysis of certain aromatic peptides a new spot was observed moving slightly faster than tyrosine in phenol and almost as fast as leucine in butanol-acetic acid. It gave a positive reaction with diazotized sulphanilic acid. In many cases pairs of peptides were found, one containing tyrosine an ...
SBT202 - SibTech, Inc.
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
Biochemistry of Fruits and Vegetables
... Enzymatic vs Alkaline Degradation of Pectin 3 reaction mechanisms here: two are chemically altering and the other is just a pectin chain breaker. Pectin lyase = transeliminase. The pectin chain MUST be methylated for this enzyme to operate and will break a pectin chain to form a double bond betw ...
... Enzymatic vs Alkaline Degradation of Pectin 3 reaction mechanisms here: two are chemically altering and the other is just a pectin chain breaker. Pectin lyase = transeliminase. The pectin chain MUST be methylated for this enzyme to operate and will break a pectin chain to form a double bond betw ...
The tyrosine regulated DAHP synthase and the biosynthetic
... can be suppressed when the genes TRP2/TRP3 encoding the anthranilate synthase complex at the initial step of the tryptophan specific branch are overexpressed. Similarly, the addition of anthranilate or tryptophan can restore growth suggesting that the phe-effect is the result of tryptophan starvatio ...
... can be suppressed when the genes TRP2/TRP3 encoding the anthranilate synthase complex at the initial step of the tryptophan specific branch are overexpressed. Similarly, the addition of anthranilate or tryptophan can restore growth suggesting that the phe-effect is the result of tryptophan starvatio ...
Analysis of Honey Samples - Research Commons@Waikato
... The HPLC-UV method involves derivatisation of amino acids by OPA-MPA and FMOC. A fully automated injection program analysed seventeen primary amino acids in 19 minutes. Ultimately, the detection by UV had inadequate sensitivity, and the secondary amino acid proline could not be detected. The method ...
... The HPLC-UV method involves derivatisation of amino acids by OPA-MPA and FMOC. A fully automated injection program analysed seventeen primary amino acids in 19 minutes. Ultimately, the detection by UV had inadequate sensitivity, and the secondary amino acid proline could not be detected. The method ...
working with peptides
... gradient can suggest high purity results when the actual purity may be much lower. Impurities consist of deletion sequences, incompletely deprotected sequences, truncated sequences and side products of the synthesis process. Residual salt and water that do not absorb at this wavelength are not quant ...
... gradient can suggest high purity results when the actual purity may be much lower. Impurities consist of deletion sequences, incompletely deprotected sequences, truncated sequences and side products of the synthesis process. Residual salt and water that do not absorb at this wavelength are not quant ...
Introduction to Carbohydrates
... Steroids with 8–10 carbon atoms in the side chain at C17 and a hydroxyl group at C-3 are classified as sterols. Cholesterol is the major sterol in animal tissues. [Note: Plant sterols, such as β-sitosterol are poorly absorbed by humans. After entering the enterocytes, they are actively transported b ...
... Steroids with 8–10 carbon atoms in the side chain at C17 and a hydroxyl group at C-3 are classified as sterols. Cholesterol is the major sterol in animal tissues. [Note: Plant sterols, such as β-sitosterol are poorly absorbed by humans. After entering the enterocytes, they are actively transported b ...
Evaluation of different fermentation processes for use by small
... acid acetic production from lactic acid bacteria Ho et al. 2014;. In addition, citric, oxalic, phosphoric, succinic, and malic acids are also produced Camu et al. 2007; Puziah et al. 1998;. Due to the exothermic nature of the above reactions, the temperature within the boxes rises up to 50°C; under ...
... acid acetic production from lactic acid bacteria Ho et al. 2014;. In addition, citric, oxalic, phosphoric, succinic, and malic acids are also produced Camu et al. 2007; Puziah et al. 1998;. Due to the exothermic nature of the above reactions, the temperature within the boxes rises up to 50°C; under ...
Folate Metabolism and MTHFR
... frequency of the TT genotype but also high rates of neural tube defect.” “…In the United States, the rates of neural tube defects historically have been higher among Hispanics, intermediate among non-Hispanic whites, and lower among African-Americans, a trend that follows the relative frequency of t ...
... frequency of the TT genotype but also high rates of neural tube defect.” “…In the United States, the rates of neural tube defects historically have been higher among Hispanics, intermediate among non-Hispanic whites, and lower among African-Americans, a trend that follows the relative frequency of t ...
Glycoside hydrolases: Catalytic base
... substrate binding or pKa modulation of either the catalytic acid or base residues, would show the same pattern of activity as a real base. Supporting evidence for the presence of a catalytic base residue in inverting GHs can be from replacement of candidate residues with cysteinesulfinate followed by ...
... substrate binding or pKa modulation of either the catalytic acid or base residues, would show the same pattern of activity as a real base. Supporting evidence for the presence of a catalytic base residue in inverting GHs can be from replacement of candidate residues with cysteinesulfinate followed by ...
Module 1. General principles of metabolism. Metabolism of
... D. It speeds up a particular chemical reaction. E. * It occurs in only one type of cell. 77. An update of Fischer's lock and key theory of enzyme specificity view the _______ as the lock and _______ as the key. A. Enzyme; substrate B. Substrate; enzyme C. * Enzyme; transition state D. Transition sta ...
... D. It speeds up a particular chemical reaction. E. * It occurs in only one type of cell. 77. An update of Fischer's lock and key theory of enzyme specificity view the _______ as the lock and _______ as the key. A. Enzyme; substrate B. Substrate; enzyme C. * Enzyme; transition state D. Transition sta ...
Regulation of Ketone Body Metabolism and the Role of PPAR
... PPARα and retinoic X receptor (RXR) associate with peroxisome proliferator response elements (PPRE) in the gene promoters. PPARα-RXR complex recruits variety of co-activator proteins with a histone acetyltransferase activity, that belong to the CBP/p300 or SRC/p160 families (reviewed in [17]). This ...
... PPARα and retinoic X receptor (RXR) associate with peroxisome proliferator response elements (PPRE) in the gene promoters. PPARα-RXR complex recruits variety of co-activator proteins with a histone acetyltransferase activity, that belong to the CBP/p300 or SRC/p160 families (reviewed in [17]). This ...
RenalTubularTransportof AminoAcids
... analyses and paper chromatography were introduced. Since then, methods have been developed and improved which have made it possible for individual amino acids to be studied rather than total a-amino-nitrogen: microbiological analyses (54-56), specific enzymic analysis (57), paper chromatography (58- ...
... analyses and paper chromatography were introduced. Since then, methods have been developed and improved which have made it possible for individual amino acids to be studied rather than total a-amino-nitrogen: microbiological analyses (54-56), specific enzymic analysis (57), paper chromatography (58- ...
Insufficiency of digestion
... • Stimulation of H2-histamine receptors is bring on formation cAMP, and stimulation of CCK-receptors and M3-receptors results to increasing of a level of endocellular calcium (Са++). Stimulation of M3-receptors increases, not only comming Са++ into a cell and due to increasing of level inositolthre ...
... • Stimulation of H2-histamine receptors is bring on formation cAMP, and stimulation of CCK-receptors and M3-receptors results to increasing of a level of endocellular calcium (Са++). Stimulation of M3-receptors increases, not only comming Са++ into a cell and due to increasing of level inositolthre ...
The Utilization of Propionate by Micrococcus
... KORNBERG, H. L. (1958). The metabolism of C,-compounds in micro-organisms. 1. The incorporation of [2-14C]acetateby Pseudomonas fluorescens,and by a Corynebacterium, grown on ammonium acetate. Biochem. J. 68, 535. KORNBERG, H. L. & MORRIS, J. G. (1965). The utilization of glycollate by Micrococcus d ...
... KORNBERG, H. L. (1958). The metabolism of C,-compounds in micro-organisms. 1. The incorporation of [2-14C]acetateby Pseudomonas fluorescens,and by a Corynebacterium, grown on ammonium acetate. Biochem. J. 68, 535. KORNBERG, H. L. & MORRIS, J. G. (1965). The utilization of glycollate by Micrococcus d ...
Exploring Yeast as a Cell Factory for the Production of Carboxylic
... substrates (biomass), for the formation of various products with commercial value. Microorganisms such as yeast or bacteria are the heart of biorefineries, since they can convert sugars present in raw materials into different forms of energy (power and heat), transportation fuels and/or chemical com ...
... substrates (biomass), for the formation of various products with commercial value. Microorganisms such as yeast or bacteria are the heart of biorefineries, since they can convert sugars present in raw materials into different forms of energy (power and heat), transportation fuels and/or chemical com ...
Production of L-(+) Lactic Acid From Blackstrap Molasses by
... LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. ...
... LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. ...
b156 - IPB Repository - Bogor Agricultural University
... Analyses of dry matters, total peptides, NaCl concentration, total sugars, total acids, pH and free l-glutamic acid concentration were performed for the three F-500 fractions and their resulting gel filtration fractions in duplicate. Dry matters of samples were analyzed by a gravimetric method and u ...
... Analyses of dry matters, total peptides, NaCl concentration, total sugars, total acids, pH and free l-glutamic acid concentration were performed for the three F-500 fractions and their resulting gel filtration fractions in duplicate. Dry matters of samples were analyzed by a gravimetric method and u ...
Peer-reviewed Article PDF
... vitamin (B1) thiamin, (B2) riboflavin, niacin and pantothenic acid. In addition, it also contains a fair amount of phosphorous, calcium, iron, potassium and sodium [2]. The high level of antioxidants pigments like carotenoids and polyphenols present in guava increases its dietary value [3]. These va ...
... vitamin (B1) thiamin, (B2) riboflavin, niacin and pantothenic acid. In addition, it also contains a fair amount of phosphorous, calcium, iron, potassium and sodium [2]. The high level of antioxidants pigments like carotenoids and polyphenols present in guava increases its dietary value [3]. These va ...
Module 2 General principles of metabolism. Мetabolism of carbohy
... D. None of the above E. Ten substrates 54. The result of a(n) __________ reaction is that energy is released. Energy must be added for a(n) __________ reaction to proceed. A. Enzyme catalyzed, non-spontaneous B. * Exergonic, endergonic C. Endergonic, spontaneous D. Catalytic, non-catalytic E. Oxidat ...
... D. None of the above E. Ten substrates 54. The result of a(n) __________ reaction is that energy is released. Energy must be added for a(n) __________ reaction to proceed. A. Enzyme catalyzed, non-spontaneous B. * Exergonic, endergonic C. Endergonic, spontaneous D. Catalytic, non-catalytic E. Oxidat ...
7 Biochemistry of fermentation
... individual metabolic pathway consists of many reactions which, in turn, are regulated by different enzyme systems, and hence it is the level of enzyme synthesis and activity which maintains and controls the functions of the microbial cell (Stanier et al., 1987). One regulatory (or feedback) mechanis ...
... individual metabolic pathway consists of many reactions which, in turn, are regulated by different enzyme systems, and hence it is the level of enzyme synthesis and activity which maintains and controls the functions of the microbial cell (Stanier et al., 1987). One regulatory (or feedback) mechanis ...
Hepoxilin
Hepoxilins (HxA3 and HxB3) are nonclassic eicosanoid hormones involved in inflammation.