This paper is published in a part-themed issue of Photochemical
... was cooled and added to the exhausted cold solution it became luminous again because the component that was used up in the cold solution was precisely the one that was not destroyed by the heat. Dubois called the molecule that was consumed in the bioluminescence reaction luciferin and the component ...
... was cooled and added to the exhausted cold solution it became luminous again because the component that was used up in the cold solution was precisely the one that was not destroyed by the heat. Dubois called the molecule that was consumed in the bioluminescence reaction luciferin and the component ...
Amino Acid and Peptide Immobilization on Oxidized Nanocellulose
... helps regenerate the catalyst TEMPO during the reaction. Using this oxidative reaction, it was found that cellulose could be completely converted into water-soluble polyglucuronic acid [17,18]. In the case of native cellulose fibers, the oxidation proceeded throughout the fibers but occurred only at ...
... helps regenerate the catalyst TEMPO during the reaction. Using this oxidative reaction, it was found that cellulose could be completely converted into water-soluble polyglucuronic acid [17,18]. In the case of native cellulose fibers, the oxidation proceeded throughout the fibers but occurred only at ...
Progress in the Correlative Atomic Force Microscopy and Optical
... between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the interactions and the elaborate structure due to the limitations of the technology, itself, such ...
... between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the interactions and the elaborate structure due to the limitations of the technology, itself, such ...
Redox speciation analysis of antimony in soil extracts by hydride
... received little environmental attention. There are only a few of studies in soils, sediments and biological materials. The natural concentration of antimony in soils is -1 mg kgy1 w2x. However, ...
... received little environmental attention. There are only a few of studies in soils, sediments and biological materials. The natural concentration of antimony in soils is -1 mg kgy1 w2x. However, ...
PDF Full-text
... a Ni complex on the surface of a substrate. An advantage of this method is that all proteins coordinate in the same direction, which prevents deactivation. It has been reported that 80% of 5800 kinds of yeast proteins maintained their activity when immobilized using this method. Katayama et al. also ...
... a Ni complex on the surface of a substrate. An advantage of this method is that all proteins coordinate in the same direction, which prevents deactivation. It has been reported that 80% of 5800 kinds of yeast proteins maintained their activity when immobilized using this method. Katayama et al. also ...
Environmental Microbiology
... 1840 M. Rothballer, M. Schmid, A. Fekete and A. Hartmann ber of root hairs and length of side roots of inoculated plants (Barbieri et al., 1986). However, it has not been possible so far to construct completely IAA-negative mutants. A strain with a knockout mutation in one of the key genes in IAA s ...
... 1840 M. Rothballer, M. Schmid, A. Fekete and A. Hartmann ber of root hairs and length of side roots of inoculated plants (Barbieri et al., 1986). However, it has not been possible so far to construct completely IAA-negative mutants. A strain with a knockout mutation in one of the key genes in IAA s ...
Document
... binding sites.3 No final consensus about the number of fatty acid binding sites has been reached so far. Under normal physiological conditions, 3–4 molecules of fatty acids combine with each HSA molecule (i.e., high affinity, low capacity binding sites). However, as many as 30 fatty acid molecules c ...
... binding sites.3 No final consensus about the number of fatty acid binding sites has been reached so far. Under normal physiological conditions, 3–4 molecules of fatty acids combine with each HSA molecule (i.e., high affinity, low capacity binding sites). However, as many as 30 fatty acid molecules c ...
REVIEW Formation and Instability of o
... The OPA independent degradation pathway appears unimportant in the presence of a moderate excess of OPA. The destabilization of OPA depends strongly on thiol structure (26). Because $ is much less variable than kl , stability differences related to structural variations may almost solely be due to i ...
... The OPA independent degradation pathway appears unimportant in the presence of a moderate excess of OPA. The destabilization of OPA depends strongly on thiol structure (26). Because $ is much less variable than kl , stability differences related to structural variations may almost solely be due to i ...
c Syun-Ru Yeh‡ and Denis L. Rousseau
... measurements suggests that under low GdnHCl concentrations, the polypeptide backbone structure changes, associated with the changes in heme ligation states, are localized and the thermodynamic intermediates are compact with most of the native secondary structure preserved. Similar conclusions were d ...
... measurements suggests that under low GdnHCl concentrations, the polypeptide backbone structure changes, associated with the changes in heme ligation states, are localized and the thermodynamic intermediates are compact with most of the native secondary structure preserved. Similar conclusions were d ...
Photoactivation mechanism of PAmCherry based on crystal
... 1.65 Å resolution, respectively (Table S1). In both isomorphous structures, the asymmetric unit contains one PAmCherry1 protein chain with total solvent accessible area of approximately 9,880 Å2 (Fig. S2). Contacts between symmetry-related protein chains are not extensive, so, in contrast to some ot ...
... 1.65 Å resolution, respectively (Table S1). In both isomorphous structures, the asymmetric unit contains one PAmCherry1 protein chain with total solvent accessible area of approximately 9,880 Å2 (Fig. S2). Contacts between symmetry-related protein chains are not extensive, so, in contrast to some ot ...
Naphtyl-imidazo-anthraquinones as novel colorimetric
... new fluorescence band. The classic design for a metal ion chemosensor consists of one or more fluorophores/chromophores linked to a coordinating unit through a spacer. The coordinating unit may be tailored by a proper choice of donor functions, able to coordinate a metal ion, and the molecular fram ...
... new fluorescence band. The classic design for a metal ion chemosensor consists of one or more fluorophores/chromophores linked to a coordinating unit through a spacer. The coordinating unit may be tailored by a proper choice of donor functions, able to coordinate a metal ion, and the molecular fram ...
Title Rapid quantification of tryptophan and tyrosine in
... complex cell culture media, we used a variety of methods. Initially, PARAFAC2 [42, 43] was implemented on dataset1 to assess the various spectral factors. This method was used in preference to PARAFAC because PARAFAC is highly dependent on data tri-linearity [44]. This means that the fluorescence re ...
... complex cell culture media, we used a variety of methods. Initially, PARAFAC2 [42, 43] was implemented on dataset1 to assess the various spectral factors. This method was used in preference to PARAFAC because PARAFAC is highly dependent on data tri-linearity [44]. This means that the fluorescence re ...
Recombinant N-terminal Nucleotide
... Multidrug resistance of tumor cells is often associated with overexpression of P-glycoprotein, a membrane transporter that extrudes chemotherapeutic drugs using ATP hydrolysis as energy source (1, 2). The protein is encoded by the mdr gene family comprising two members in man, mdr1 and mdr2, or thre ...
... Multidrug resistance of tumor cells is often associated with overexpression of P-glycoprotein, a membrane transporter that extrudes chemotherapeutic drugs using ATP hydrolysis as energy source (1, 2). The protein is encoded by the mdr gene family comprising two members in man, mdr1 and mdr2, or thre ...
Long-chain fatty acids increase basal metabolism
... rise in the monomer signal when the ⌬⌿ of a JC-1-loaded myocyte was dissipated (Fig. 2). This observation is consistent with the previous report that the monomer form of JC-1 is sensitive to a lower range of ⌬⌿ than the J aggregate form (7). Hence, because JC-1 does not behave as a typical ratioable ...
... rise in the monomer signal when the ⌬⌿ of a JC-1-loaded myocyte was dissipated (Fig. 2). This observation is consistent with the previous report that the monomer form of JC-1 is sensitive to a lower range of ⌬⌿ than the J aggregate form (7). Hence, because JC-1 does not behave as a typical ratioable ...
Green Fluorescent Protein
... • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo • Renaturation most likely by a parallel pathway • Oxidation of Fluoropore (2-4 hours) • Two step process ...
... • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo • Renaturation most likely by a parallel pathway • Oxidation of Fluoropore (2-4 hours) • Two step process ...
The transcriptional regulator CprK detects chlorination by combining
... Desulfitobacterium species are able to dechlorinate a range of halogen-substituted phenolic compounds [9–11]. In D. dehalogenans and D. hafniense, the CprA genes are under tight transcriptional control by the regulator CprK [9]. CprK belongs to the CRP/FNR family of regulators and, following binding ...
... Desulfitobacterium species are able to dechlorinate a range of halogen-substituted phenolic compounds [9–11]. In D. dehalogenans and D. hafniense, the CprA genes are under tight transcriptional control by the regulator CprK [9]. CprK belongs to the CRP/FNR family of regulators and, following binding ...
Journal of Agricultural and Food Chemistry
... Amaranth and quinoa were ground in a mill with a 60-mesh screen and defatted in a Soxhlet extractor with a ratio of n-hexane (boiling point 69 °C) to sample of 30:1 (v/w) for 10 h. The meal was stored at 4 °C after removal of n-hexane. Amaranth and quinoa albumins (Alb) and globulins (G) were extrac ...
... Amaranth and quinoa were ground in a mill with a 60-mesh screen and defatted in a Soxhlet extractor with a ratio of n-hexane (boiling point 69 °C) to sample of 30:1 (v/w) for 10 h. The meal was stored at 4 °C after removal of n-hexane. Amaranth and quinoa albumins (Alb) and globulins (G) were extrac ...
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... sphere. We calculate the fluorescent DPDW generated by a point source inside and outside the sphere and then integrate the fluorescent DPDW over the corresponding source distribution to obtain the total fluorescent DPDW. Before proceeding, we specify the following notation convention: All background ...
... sphere. We calculate the fluorescent DPDW generated by a point source inside and outside the sphere and then integrate the fluorescent DPDW over the corresponding source distribution to obtain the total fluorescent DPDW. Before proceeding, we specify the following notation convention: All background ...
Development of a Silica Surface Modified with Reactive Amino
... We previously reported that the surfaces of silica particles modified with aminosilane could be rapidly immobilized with single-stranded nucleic acids in PBS solution at 37 °C to prevent their denaturation.(4) The single-stranded nucleic acids trapped at the modified silica surfaces by hydrogen bo ...
... We previously reported that the surfaces of silica particles modified with aminosilane could be rapidly immobilized with single-stranded nucleic acids in PBS solution at 37 °C to prevent their denaturation.(4) The single-stranded nucleic acids trapped at the modified silica surfaces by hydrogen bo ...
SCINDAPSUS OFFICINALIS (ROXB.) SCHOTT. (ARACEAE) Research Article MALARKODI VELRAJ,
... elemental study, metal study, preliminary phytochemical screening and fluorescence analysis have been described. The powder microscopical study reveals the presence of endosperm cells with starch grains inside, lignified sclereids and fragmented perienth. The total ash, acid insoluble ash, water sol ...
... elemental study, metal study, preliminary phytochemical screening and fluorescence analysis have been described. The powder microscopical study reveals the presence of endosperm cells with starch grains inside, lignified sclereids and fragmented perienth. The total ash, acid insoluble ash, water sol ...
SOIL PENETROMETER WITH RAMAN SAMPLING
... with the uncooled camera with one second of exposure at 25 ºC............................ 84 5.15 Monochrome image an Antarctica rock with an endolithic bacteria layer. This image was taken with the uncooled camera with one second of exposure at 25 ºC................................................. ...
... with the uncooled camera with one second of exposure at 25 ºC............................ 84 5.15 Monochrome image an Antarctica rock with an endolithic bacteria layer. This image was taken with the uncooled camera with one second of exposure at 25 ºC................................................. ...
whole-cell based fluorescence biosensors for environmental
... types of pollutants in the environment [18,19]. These features make them the ideal candidates for incorporation into biosensors. II. OPTICAL BIOSENSORS: APPLICATIONS IN ENVIRONMENTAL ASSESSMENT C. Fluorescence-based Biosensors The emission of fluorescence is a result of electrons that have been exci ...
... types of pollutants in the environment [18,19]. These features make them the ideal candidates for incorporation into biosensors. II. OPTICAL BIOSENSORS: APPLICATIONS IN ENVIRONMENTAL ASSESSMENT C. Fluorescence-based Biosensors The emission of fluorescence is a result of electrons that have been exci ...
Oxidized Amino Acids in Lens Protein with Age
... constructed using 0-2.5 ng of DT. The DTcontent of the protein was pmol of DT/mol of Tyr in the oxidized model proteins. For normalized to the content of the precursor amino acid Tyr, determined by amino acid analysis of the hydrolysate (9). As described this reason, a SIM-GC/MS assay was developedf ...
... constructed using 0-2.5 ng of DT. The DTcontent of the protein was pmol of DT/mol of Tyr in the oxidized model proteins. For normalized to the content of the precursor amino acid Tyr, determined by amino acid analysis of the hydrolysate (9). As described this reason, a SIM-GC/MS assay was developedf ...
FRET!
... various fluorescent colors (GFP, CFP, YFP, …). They have overlapping spectra and can transfer excitation directly by FRET when the proteins are close together. The energy transfer occurs without a photon. ...
... various fluorescent colors (GFP, CFP, YFP, …). They have overlapping spectra and can transfer excitation directly by FRET when the proteins are close together. The energy transfer occurs without a photon. ...
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. However, unlike phosphorescence, where the substance would continue to glow and emit light for some time after the radiation source has been turned off, fluorescent materials would cease to glow immediately upon removal of the excitation source. Hence, it is not a persistent phenomenon.Fluorescence has many practical applications, including mineralogy, gemology, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom.