Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
... structures are used as capture agents. Interactions are reported by environmentally sensitive fluorophores attached to the peptides. Libraries of peptides with β-strand (16 peptides, [25]), β-loop (126 peptides, [24]) and -helix (20 peptides, [27]) structures were synthesized. In proteins, the solv ...
... structures are used as capture agents. Interactions are reported by environmentally sensitive fluorophores attached to the peptides. Libraries of peptides with β-strand (16 peptides, [25]), β-loop (126 peptides, [24]) and -helix (20 peptides, [27]) structures were synthesized. In proteins, the solv ...
Photosynthesis
... Accessory pigments, such as chlorophyll b, broaden the spectrum used for photosynthesis Accessory pigments called carotenoids absorb excessive light that would damage chlorophyll ...
... Accessory pigments, such as chlorophyll b, broaden the spectrum used for photosynthesis Accessory pigments called carotenoids absorb excessive light that would damage chlorophyll ...
Fluorescence of coumarins and xanthenes after two
... side on2. The spectral bandwidth is 8 nm. Since the natural bandwidth of the fluorescence spectra is 30–50-nm error in determining the true peak height is less than 5%.11 The signal of the photomultiplier tube 1PMT2 was amplified 1Hamamatsu C 1053-12 and measured with an analog-to-digital–digital-to ...
... side on2. The spectral bandwidth is 8 nm. Since the natural bandwidth of the fluorescence spectra is 30–50-nm error in determining the true peak height is less than 5%.11 The signal of the photomultiplier tube 1PMT2 was amplified 1Hamamatsu C 1053-12 and measured with an analog-to-digital–digital-to ...
Properties of a 4Pi confocal fluorescence microscope
... illumination photon's both exciting a fluorophore in the vicinity of the geometrical focus and detecting a photon emitted from the same position. 20 The basic idea of the 4Pi confocal fluorescence microscope is to use two microscope objectives with a common focus to increase the aperture of the micr ...
... illumination photon's both exciting a fluorophore in the vicinity of the geometrical focus and detecting a photon emitted from the same position. 20 The basic idea of the 4Pi confocal fluorescence microscope is to use two microscope objectives with a common focus to increase the aperture of the micr ...
Green Fluorescent Protein
... The Green fluorescent protein (GFP) was first isolated from the Jellyfish Aequorea victoria, widely found in North America. GFP has existed for more than one hundred and sixty million years in that species. GFP is a widely studied and highly exploited protein in the fields of biochemistry and cell b ...
... The Green fluorescent protein (GFP) was first isolated from the Jellyfish Aequorea victoria, widely found in North America. GFP has existed for more than one hundred and sixty million years in that species. GFP is a widely studied and highly exploited protein in the fields of biochemistry and cell b ...
Enzymes Detection
... ADIFAB and ADIFAB2 are ideal also for drug screening. They are particularly well-suited for drugs that affect cellular processes as well as those involving purified enzymes. FFAu probes have been validated for high throughput assay. This includes robotic dispensing of reagents, fluorescence screenin ...
... ADIFAB and ADIFAB2 are ideal also for drug screening. They are particularly well-suited for drugs that affect cellular processes as well as those involving purified enzymes. FFAu probes have been validated for high throughput assay. This includes robotic dispensing of reagents, fluorescence screenin ...
Fluorescent Amino Acids: Modular Building Blocks for the Assembly
... shows a modest increase in quantum yield together with a significant blue shift of the emission λmax of up to ~130 nm when changing exposure from aqueous protic to organic aprotic solvents.29 An α-amino acid building block containing this fluorophore, 6-(2dimethylaminonaphthoyl) alanine (DANA) was s ...
... shows a modest increase in quantum yield together with a significant blue shift of the emission λmax of up to ~130 nm when changing exposure from aqueous protic to organic aprotic solvents.29 An α-amino acid building block containing this fluorophore, 6-(2dimethylaminonaphthoyl) alanine (DANA) was s ...
physiological limitations on phytoplankton productivity in the ocean
... pump-and-probe fluorescence technique (Falkowski et al., 1986), in which the change in the quantum yield of fluorescence (Aq~) for a weak "probe" flash is measured before (F0) and after (Fs) a stimulating (actinic) " p u m p " flash, Aq5 = (Fs - Fo)/Fo. The technique is rapid, nondestructive, and is ...
... pump-and-probe fluorescence technique (Falkowski et al., 1986), in which the change in the quantum yield of fluorescence (Aq~) for a weak "probe" flash is measured before (F0) and after (Fs) a stimulating (actinic) " p u m p " flash, Aq5 = (Fs - Fo)/Fo. The technique is rapid, nondestructive, and is ...
The role of aromatic side chain residues in micelle binding by
... designated as the 'tyrosine loop' region [6]. Conformation analyses of colipase have been performed using a variety of biophysical techniques, including u.v. spectroscopy, c.d. and n.m.r. techniques, the latter indicating that one of the 'loop' tyrosyl residues is restricted in its motion [9-11]. Ph ...
... designated as the 'tyrosine loop' region [6]. Conformation analyses of colipase have been performed using a variety of biophysical techniques, including u.v. spectroscopy, c.d. and n.m.r. techniques, the latter indicating that one of the 'loop' tyrosyl residues is restricted in its motion [9-11]. Ph ...
2 - Durham Research Online
... time-resolution ∆t of up to a few femto seconds, which is mainly limited by the temporal width ∆τp of the laser pulse. The laser source is the same system that was used for the ultra fast transient absorption measurements (see previous paragraph). Our cross-linear fluorescence up-conversion setup is ...
... time-resolution ∆t of up to a few femto seconds, which is mainly limited by the temporal width ∆τp of the laser pulse. The laser source is the same system that was used for the ultra fast transient absorption measurements (see previous paragraph). Our cross-linear fluorescence up-conversion setup is ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
... Fig. 2. The FluoroVueTM Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. Caution Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solutio ...
... Fig. 2. The FluoroVueTM Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. Caution Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solutio ...
Joint Symposium of Waseda University and Peking University
... water/1,2-dichloroethane (W/DCE) interface. The IT and FIT reactions of protonated dopamine can be observed simultaneously within the same potential window. The experimental results demonstrate that dibenzo-18-crown-6 (DB18C6), dibenzo-24-crown-8 (DB24C8), benzo-15-crown-5 (B15C5) work well with the ...
... water/1,2-dichloroethane (W/DCE) interface. The IT and FIT reactions of protonated dopamine can be observed simultaneously within the same potential window. The experimental results demonstrate that dibenzo-18-crown-6 (DB18C6), dibenzo-24-crown-8 (DB24C8), benzo-15-crown-5 (B15C5) work well with the ...
REAL-TIME PCR
... reduces the fluorescence emitted by the reporter dye from the second probe using the fluorescence resonance energy transfer (FRET). The FRET phenomenon is distance-dependent, meaning this process will only take place when the two dyes are in close proximity. FRET occurs due to interaction between th ...
... reduces the fluorescence emitted by the reporter dye from the second probe using the fluorescence resonance energy transfer (FRET). The FRET phenomenon is distance-dependent, meaning this process will only take place when the two dyes are in close proximity. FRET occurs due to interaction between th ...
Integrity and purity of the mitochondrial fraction
... image (Fig. S8C, upper left panel). Nuclear masks were performed manually (Fig. S8C, lower left panel). hERK1-GFP kinetics were studied only in mitochondria of transfected cells, and thus a new mask was determined by the combination of both the cellular mask and the MitoTracker generated mask (Fig. ...
... image (Fig. S8C, upper left panel). Nuclear masks were performed manually (Fig. S8C, lower left panel). hERK1-GFP kinetics were studied only in mitochondria of transfected cells, and thus a new mask was determined by the combination of both the cellular mask and the MitoTracker generated mask (Fig. ...
CDTE Quantum Dots – Preparation, Physico
... during one synthesis. The refluxing time of last reaction stage determine the size and emission maximum of rising nanoparticles [14]. Several samples were taken during refluxing in different times. The fluorescence spectra of these samples were measured and emission maxima and bandwidths were determ ...
... during one synthesis. The refluxing time of last reaction stage determine the size and emission maximum of rising nanoparticles [14]. Several samples were taken during refluxing in different times. The fluorescence spectra of these samples were measured and emission maxima and bandwidths were determ ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
... presence of an energy barrier between a ππ* excited state and the ground state.11 This barrier may form a minimum well in the excited-state potential energy of 2AP that is below the intersection point with the ground-state potential energy surface and from where the fluorescence process can take plac ...
... presence of an energy barrier between a ππ* excited state and the ground state.11 This barrier may form a minimum well in the excited-state potential energy of 2AP that is below the intersection point with the ground-state potential energy surface and from where the fluorescence process can take plac ...
TriStar² LB 942 - Berthold Technologies USA
... Especially BRET/BRET² and other colour luminescence applications are only possible with an extremely efficient optical system together with appropriate filters. Up to 30 different excitation and emission filters can be easily mounted on exchangeable filter carriers. These carriers are software-drive ...
... Especially BRET/BRET² and other colour luminescence applications are only possible with an extremely efficient optical system together with appropriate filters. Up to 30 different excitation and emission filters can be easily mounted on exchangeable filter carriers. These carriers are software-drive ...
SPECTROSCOPIC MANIFESTATION OF DYE PAIR
... (SRSF) was the purpose of the given research. SRSF nanometrical thickness were prepared by the sol-gel technology with use the surfactant Pluronic P123.The hydrophobic nature of the surfactant can be utilized for the strong solubilization of organic compounds and the one-dimensionality enables us to ...
... (SRSF) was the purpose of the given research. SRSF nanometrical thickness were prepared by the sol-gel technology with use the surfactant Pluronic P123.The hydrophobic nature of the surfactant can be utilized for the strong solubilization of organic compounds and the one-dimensionality enables us to ...
spectroscopic studies of mosquito iridescent virus, its capsid
... determined mainly by the individual properties of their π-electron systems, such as aromatic amino acids (tryptophan, tyrosine and phenylalanine), which are centers of intense UV absorption and fluorescence (λ > 200 nm). A success has been reached in modeling the spectra of large and complex native ...
... determined mainly by the individual properties of their π-electron systems, such as aromatic amino acids (tryptophan, tyrosine and phenylalanine), which are centers of intense UV absorption and fluorescence (λ > 200 nm). A success has been reached in modeling the spectra of large and complex native ...
Chem 301 Biological Chemistry I Laboratory Lab 7: Protein
... order to characterize proteins with respect to their structure, the nature of their interactions with other molecules (e.g. DNA, other proteins, and small molecule ligands and reaction substrates), their local solvent environment, conformational changes that they undergo, and many other aspects of t ...
... order to characterize proteins with respect to their structure, the nature of their interactions with other molecules (e.g. DNA, other proteins, and small molecule ligands and reaction substrates), their local solvent environment, conformational changes that they undergo, and many other aspects of t ...
Chapter 5 Section 2 Notes
... - phosphorescence – ability of a mineral to continue to glow after ultraviolet light has been removed Chatoyancy and Asterism - chatoyancy – minerals that display a silky appearance in reflected light o “cats-eye-effect” o a result of closely packed parallel fibers - asterism – a six sided star shap ...
... - phosphorescence – ability of a mineral to continue to glow after ultraviolet light has been removed Chatoyancy and Asterism - chatoyancy – minerals that display a silky appearance in reflected light o “cats-eye-effect” o a result of closely packed parallel fibers - asterism – a six sided star shap ...
Comparing Sequences of Fluorescent Proteins Using
... research (see image at right). This diversity of colors among fluorescent proteins has sometimes been referred to as the “mFruits,” referring to the names given to these fluorescent proteins, such as: • mBlueberry (Blue Fluorescent Protein, or BFP) • mLemon (Yellow Fluorescent Protein, or YFP) • mGr ...
... research (see image at right). This diversity of colors among fluorescent proteins has sometimes been referred to as the “mFruits,” referring to the names given to these fluorescent proteins, such as: • mBlueberry (Blue Fluorescent Protein, or BFP) • mLemon (Yellow Fluorescent Protein, or YFP) • mGr ...
Hundred-thousand light holes push nanoscopy to go parallel
... modulation and single-molecule localization, such as (f)PALM/STORM (Betzig et al. 2006; Hess, Girirajan, and Mason 2006; Rust, Bates, and Zhuang 2006) in which the photo-switch nature of the fluorescent molecule is employed to achieve single-molecule imaging. Of these methods, STED is more commonly ...
... modulation and single-molecule localization, such as (f)PALM/STORM (Betzig et al. 2006; Hess, Girirajan, and Mason 2006; Rust, Bates, and Zhuang 2006) in which the photo-switch nature of the fluorescent molecule is employed to achieve single-molecule imaging. Of these methods, STED is more commonly ...
IX71/IX81 - Olympus Microscopy Resource Center
... wheels on the excitation and absorption sides. However, this kind of mirror encounters the problem that each fluorescence image gets darker as the number of color dyes increase, because the transmission spectrum becomes narrower and the transmittance falls to lower than 90% at best. Olympus has ther ...
... wheels on the excitation and absorption sides. However, this kind of mirror encounters the problem that each fluorescence image gets darker as the number of color dyes increase, because the transmission spectrum becomes narrower and the transmittance falls to lower than 90% at best. Olympus has ther ...
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. However, unlike phosphorescence, where the substance would continue to glow and emit light for some time after the radiation source has been turned off, fluorescent materials would cease to glow immediately upon removal of the excitation source. Hence, it is not a persistent phenomenon.Fluorescence has many practical applications, including mineralogy, gemology, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom.