Photosynthesis - mleonessciencepage
... Capturing the Light “Light dependent reactions” take place in chloroplast found in mesophyll of leaf. ...
... Capturing the Light “Light dependent reactions” take place in chloroplast found in mesophyll of leaf. ...
SPECTROPHOTOMETRY
... Spectrophotometry • The amino acids tryptophan, tyrosine & cytosine absorb light in the UV wavelength • Aromatic rings in the bases of nucleic acids also absorb light in the UV range ...
... Spectrophotometry • The amino acids tryptophan, tyrosine & cytosine absorb light in the UV wavelength • Aromatic rings in the bases of nucleic acids also absorb light in the UV range ...
Photosynthesis Light-Dependent Reactions Calvin Cycle
... C4 Photosynthesis : C4 plants. • Called C4 because the CO2 is first incorporated into a 4carbon compound. • Stomata are open during the day. Adaptive Value: • Photosynthesizes faster than C3 plants under high light intensity and high temperatures • Has better Water Use Efficiency because they do no ...
... C4 Photosynthesis : C4 plants. • Called C4 because the CO2 is first incorporated into a 4carbon compound. • Stomata are open during the day. Adaptive Value: • Photosynthesizes faster than C3 plants under high light intensity and high temperatures • Has better Water Use Efficiency because they do no ...
Two-Color Two-Photon Excitation Using Femtosecond Laser Pulses
... microscopy. We demonstrate measurements of 2c2p excitation fluorescence using femtosecond pulses from a Ti:Sa laser. Since these experiments were carried out using a time-gated camera system as a detector which is normally used for fluorescence lifetime imaging, we believe that applications of 2c2p ...
... microscopy. We demonstrate measurements of 2c2p excitation fluorescence using femtosecond pulses from a Ti:Sa laser. Since these experiments were carried out using a time-gated camera system as a detector which is normally used for fluorescence lifetime imaging, we believe that applications of 2c2p ...
Denaturation of hemoglobin in the presence of tannic acid
... Tannic acid (TA) is a natural gallotannin and is comprised of a pentagalloylglucose core esterified at all functional hydroxyl groups with gallic acid molecules (see Scheme) [4]. TA belongs to the class of hydrolysable tannins and is found in numerous plants and foods. It generally can be extracted ...
... Tannic acid (TA) is a natural gallotannin and is comprised of a pentagalloylglucose core esterified at all functional hydroxyl groups with gallic acid molecules (see Scheme) [4]. TA belongs to the class of hydrolysable tannins and is found in numerous plants and foods. It generally can be extracted ...
Synechocystis sp
... protein, and in Synechocystis sp. PCC 6803, a cyanobacterium which lack a gene that encodes D1r protein (used as a control). We also show variation in the number active centers number of PSII during the Light/Dark cycles, recorded as a reversible decrease of fluorescence amplitude. This results are ...
... protein, and in Synechocystis sp. PCC 6803, a cyanobacterium which lack a gene that encodes D1r protein (used as a control). We also show variation in the number active centers number of PSII during the Light/Dark cycles, recorded as a reversible decrease of fluorescence amplitude. This results are ...
Document
... light absorbed is sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
... light absorbed is sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
Document
... light absorbed is sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
... light absorbed is sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
Raman Spectroscopy
... sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
... sufficiently high the electrons may not only jump to the outer levels but may leave the atoms completely ; the process is called photoelectric effect. Thus photophysical processes include phenomena like fluorescence , phosphresence and photoelectric effect. ...
Fluorescence Polarization Detection of Brucella abortus
... Both of these agents have the ability to infect a number of other animals including humans. The presence of antibodies against these pathogens in bovine serum is indicative of infection. Fluorescence polarization (FP) is a fluorescence detection technique first described in 1926 by Perrin. It is bas ...
... Both of these agents have the ability to infect a number of other animals including humans. The presence of antibodies against these pathogens in bovine serum is indicative of infection. Fluorescence polarization (FP) is a fluorescence detection technique first described in 1926 by Perrin. It is bas ...
Photofragmentation-laser induced fluorescence: a
... Although the conventional LIEF technique can be expected to provide an effective means of detecting numerous trace gases at natural tropospheric concentration levels, a great many others are not detectable by this method due to the absence of bonding excited states that fluoresce. In the latter case ...
... Although the conventional LIEF technique can be expected to provide an effective means of detecting numerous trace gases at natural tropospheric concentration levels, a great many others are not detectable by this method due to the absence of bonding excited states that fluoresce. In the latter case ...
Spectroscopic Techniques: I Spectrophotometric Techniques
... When considering a diatomic molecule (see Fig. 12.3), rotational and vibrational levels possess discrete energies that only merge into a continuum at very high energy. Each electronic state of a molecule possesses its own set of rotational and vibrational levels. Since the kind of schematics shown i ...
... When considering a diatomic molecule (see Fig. 12.3), rotational and vibrational levels possess discrete energies that only merge into a continuum at very high energy. Each electronic state of a molecule possesses its own set of rotational and vibrational levels. Since the kind of schematics shown i ...
Methods in Cell Biology
... which stains positively charged molecules (pink) • Cytoplasm (proteins) ...
... which stains positively charged molecules (pink) • Cytoplasm (proteins) ...
S U P E R -R E S O LV... Scientific Background on the Nobel Prize in Chemistry 2014
... 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have been imp ...
... 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have been imp ...
super-resolved fluorescence microscopy
... 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have been imp ...
... 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have been imp ...
Correlated diffraction and fluorescence in the backscattering
... The “birdwings” essentially collect the genera Ornitopthera, Trogonoptera, and Troides 关2–4兴, all placed by animal systematics in the tribe Troidini of the family of true butterflies 共Papilionidae兲. With respect to larval host, Troidini offer the best examples of “oligophagy” in butterflies, which m ...
... The “birdwings” essentially collect the genera Ornitopthera, Trogonoptera, and Troides 关2–4兴, all placed by animal systematics in the tribe Troidini of the family of true butterflies 共Papilionidae兲. With respect to larval host, Troidini offer the best examples of “oligophagy” in butterflies, which m ...
Single Photon Sources - University of Rochester
... begin oscillating. Once these two requirements have been filled, the cantilever is ready to begin analyzing the surface of the sample, initiated with the “scan” button on the computer screen. 3.3 Fluorescence lifetime Fluorescence lifetime is obtained with a similar setup as the determination of ant ...
... begin oscillating. Once these two requirements have been filled, the cantilever is ready to begin analyzing the surface of the sample, initiated with the “scan” button on the computer screen. 3.3 Fluorescence lifetime Fluorescence lifetime is obtained with a similar setup as the determination of ant ...
Chapter 5 MICROSCOPY: IMAGING OF BIOLOGICAL SPECIMENS
... a second longer wavelength fluorophore (the acceptor). Note that the transfer can also be considered a quenching reaction because there is no emission of fluorescence by the donor fluorophore. As outlined in Chapter 3, FRET is also used in real-time PCR. In general, the proteins to be studied using ...
... a second longer wavelength fluorophore (the acceptor). Note that the transfer can also be considered a quenching reaction because there is no emission of fluorescence by the donor fluorophore. As outlined in Chapter 3, FRET is also used in real-time PCR. In general, the proteins to be studied using ...
Fluorescence Fidelity Depends on Filters
... Fluorescence microscopy is a ubiquitous and continuously evolving technique which permits one to peer into the biological world at the micron length scale and beyond, allowing direct access into the spatial location and behavior of small molecules at the cellular level. This level of performance can ...
... Fluorescence microscopy is a ubiquitous and continuously evolving technique which permits one to peer into the biological world at the micron length scale and beyond, allowing direct access into the spatial location and behavior of small molecules at the cellular level. This level of performance can ...
Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence
... resistance to photobleaching in comparison to that of iRFP670 (Fig. 3C). GAF-FP had the complex photobleaching kinetics with two components, the first photobleached within ~200 s, reaching of 80% of initial fluorescence level, while the second remained fluorescent throughout long light exposures (> ...
... resistance to photobleaching in comparison to that of iRFP670 (Fig. 3C). GAF-FP had the complex photobleaching kinetics with two components, the first photobleached within ~200 s, reaching of 80% of initial fluorescence level, while the second remained fluorescent throughout long light exposures (> ...
Monomeric Cyanine Nucleic Acid Stains
... Allow all solutions to warm to room temperature and mix thoroughly before use. ...
... Allow all solutions to warm to room temperature and mix thoroughly before use. ...
REAL-TIME PCR
... dye on the 5’ end and a non-fluorescent quencher dye on the 3’ end. The most commonly used hydrolysis probes are TaqMan® probes (Holland et al., 1991; Heid, 1996). Molecular beacons probes (Tyagi and Kramer, 1996; Tyagi et al., 1998) and Scorpions primers (Whitcombe et al., 1999; Thelwell et al., 20 ...
... dye on the 5’ end and a non-fluorescent quencher dye on the 3’ end. The most commonly used hydrolysis probes are TaqMan® probes (Holland et al., 1991; Heid, 1996). Molecular beacons probes (Tyagi and Kramer, 1996; Tyagi et al., 1998) and Scorpions primers (Whitcombe et al., 1999; Thelwell et al., 20 ...
Atomic emission spectrum
... This line spectrum is also called the Atomic Spectrum because it originates in the element. Each element has a different atomic spectrum.The production of line spectra by the atoms of an element, indicates that an atom can radiate only certain amount of energy. This leads to the conclusion that e ...
... This line spectrum is also called the Atomic Spectrum because it originates in the element. Each element has a different atomic spectrum.The production of line spectra by the atoms of an element, indicates that an atom can radiate only certain amount of energy. This leads to the conclusion that e ...
Photosynthesis
... Electromagnetic Radiation Travels in waves The shorter the wavelength (λ, nm), the more energy Behaves like particles, called Photons (no mass) Plants have receptive molecules (pigments) that only λ receive certain photons ...
... Electromagnetic Radiation Travels in waves The shorter the wavelength (λ, nm), the more energy Behaves like particles, called Photons (no mass) Plants have receptive molecules (pigments) that only λ receive certain photons ...
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. However, unlike phosphorescence, where the substance would continue to glow and emit light for some time after the radiation source has been turned off, fluorescent materials would cease to glow immediately upon removal of the excitation source. Hence, it is not a persistent phenomenon.Fluorescence has many practical applications, including mineralogy, gemology, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom.