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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2011
BT 1822 - IMMUNOLOGY & IMMUNOTECHNOLOGY
Date : 09-11-2011
Time : 1:00 - 4:00
Dept. No.
PART – A
Answer all the questions
Max. : 100 Marks
(20 marks)
1. Choose the correct answer:
(5×1 = 5 marks)
(1) Which one of the following is not an antigen presenting cell?
(a) B cell (b) Macrophage (c) Mast cell (d) Dendritic cell
(2) Which one of the following has antiviral activity?
(a) Interferons (b) Interleukins (c) Cytokines (d) Chemokines
(3) Tissue typing can be done using
(a) Immunodiffusion (b) Mixed lymphocyte reaction (c) Agglutination (d) Elispot assay
(4) Which one of the following provides active immunity?
(a) antitoxins
(b) breast milk
(c) vaccines
(d) maternal antibody
(5) Which one of the following technique is used for counting different types of cells?
(a) Flow cytometry (b) Immunochromatography (c) Western blotting (d) Agglutination
II. State whether the following are true or false, if false give reason:
(5×1= 5 marks)
(6) T lymphocytes recognize antigen only when it is bound to a MHC molecule.
(7) Antibody dependent cellular cytotoxicity is triggered
trigg
by mast cells.
(8) The organ donor has to be fully HLA-compatible
HLA
for successful transplantation.
(9) Polyethylene glycol is used for hybridoma production.
(10) TMB/H2O2 can be used as a substrate in ELISA.
III. Complete the following:
(5×1 = 5 marks)
(11) _______ are non-phagocytic
phagocytic granulocytes.
(12) _______ is a generalized autoimmune disorder.
(13 ________ graft rejection occurs months or years after transplantation.
(14) Hybridoma cells cannot grow in the absence of ______ and ______ in the medium.
(15) _________ membrane is used for immunoscreening of recombinant libraries.
IV. Answer the following questions, each within 50 words only
(16)
(17)
(18)
(19)
(20)
What are Peyer’s patches?
Distinguish between affinity and avidity of an antibody.
Define antigenic shift.
What are catalytic antibodies?
Give two disadvantages in the validation of immunoassays.
(5×1 = 5 marks)
PART – B
(5×8 = 40 marks)
Answer the following questions, each within 500 words; Draw diagrams wherever necessary.
21(a) Explain cell-mediated immunity.
OR
(b) Describe the nature of antigens and add a note on superantigens.
22(a) Give an account of organ-specific autoimmune disorders caused by direct cellular damage.
OR
(b) Explain type III hypersensitive reactions.
23(a) Discuss the different methods of tissue typing.
OR
(b) Write notes on:
(i) Viral evasion of the immune system
(ii) Types of tumour antigens
24(a) Write notes on subunit and DNA vaccines.
OR
(b) Explain the production of an attenuated vaccine for Vibrio cholerae.
25(a) Write notes on:
(i) Epitope mapping
(5)
(ii) Immunochromatography (3)
OR
(b) Describe the technique of flow cytometry.
(2×20 = 40 marks)
PART – C
Answer any two of the following questions, each within 1500 words; Draw diagrams wherever necessary.
(26) Write notes on the production and applications of mouse monoclonal antibodies.
(27) Explain the structure and functions of the secondary lymphoid organs.
(28) Describe the following:
(i) Antibody engineering in E. coli (10)
(ii) Mechanism of graft rejection (10)
(29) Discuss the different pathways of processing and presentation of protein antigens.
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