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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIOMED.INSTRUMENTATION SC. THIRD SEMESTER – NOVEMBER 2009 BI 3810 / 3806 - TECHNICAL PATHOLOGY Date & Time: 05/11/2009 / 9:00 - 12:00 Dept. No. Max. : 100 Marks PART-A Answer ALL the questions (10 X 2 =20) 1. Enumerate the factors that increase the risk of folliculities. 2. Mention the two important methods of sharpening? 3. Give the importance of histochemistry. 4. Define Epstein Barr virus. 5. What is lipofuscin pigment? 6. Name a few proteins involved in amyloid process 7. Categorize staining methods? 8. Differentiate between atrophy from hypertrophy. 9. What is xeroderma pigmentosum? 10. State the importance of Flowcytometry. PART-B Answer any FOUR (4 X 10 =40) 11. Explain the four basic types of melanoma and add a note on diagnosis and treatment. 12. How will you estimate the amount of amino acids present in a biological sample? 13. Explain the microtome which is required for immediate observation of tissue in hospital. 14. Elaborate in detail molecular mechanism of Apoptosis. 15. Describe the popular histochemical staining technique to demonstrate carbohydrate. 16. Explain the sequence of events in irreversible cell injury in ischaemia with a flow chart. PART-C Answer any TWO (2 X 20 = 40) 17. Explain the metabolic steps and the pathological alterations involved in fatty changes in the liver. 18. How will you diagnose eczema? And explain the treatment, precaution and remedies. 19. Write down the steps of “Paraffin embedding technique” for section cutting with suitable diagrams. 20. Write notes on a) PTAH b) DNA C) Alcian blue d) Sudan black B ********** 1