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Culturing Viruses in the Laboratory Culturing Viruses in the Laboratory • Culturing Viruses in Mature Organisms – In bacteria – In plants and animals 10/15/11 MDufilho 2 Culturing Viruses in the Laboratory • Culturing Viruses in Embryonated Chicken Eggs – Inexpensive, among the largest of cells, free of contaminating microbes, and contain a nourishing yolk 10/15/11 MDufilho 3 Culturing Viruses in the Laboratory • Culturing Viruses in Cell (Tissue) Culture – Consists of cells isolated from an organism and grown on a medium or in a broth – Two types of cell cultures • Diploid cell cultures • Continuous cell cultures 10/15/11 MDufilho 4 Are Viruses Alive? • Some consider them complex pathogenic chemicals • Others consider them the least complex living entities – Use sophisticated methods to invade cells – Have the ability to take control of their host cell – Are able to replicate themselves 10/15/11 MDufilho 5