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Transcript
Culturing Viruses in the
Laboratory
Culturing Viruses in the Laboratory
• Culturing Viruses in
Mature Organisms
– In bacteria
– In plants and
animals
10/15/11
MDufilho
2
Culturing Viruses in the Laboratory
• Culturing Viruses
in Embryonated
Chicken Eggs
– Inexpensive,
among the largest
of cells, free of
contaminating
microbes, and
contain a
nourishing yolk
10/15/11
MDufilho
3
Culturing Viruses in the Laboratory
• Culturing Viruses in
Cell (Tissue) Culture
– Consists of cells
isolated from an
organism and grown
on a medium or in a
broth
– Two types of cell
cultures
• Diploid cell cultures
• Continuous cell
cultures
10/15/11
MDufilho
4
Are Viruses Alive?
• Some consider them complex pathogenic
chemicals
• Others consider them the least complex living
entities
– Use sophisticated methods to invade cells
– Have the ability to take control of their host cell
– Are able to replicate themselves
10/15/11
MDufilho
5