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DNA Technology
This involves Restriction enzymes: Enzymes that recognize a DNA sequence (usually a
palindrome) and cut DNA at that sequence. ( p. 398 in textbook)
Two major types of DNA Technology
A. Gene Splicing: DNA from one organism is spliced into another organism
(bacteria) (see p. 397-399)
(Recombinant DNA or gene cloning)
Examples:
 To make human proteins Ex. Insulin, HGH
 To make genetically altered organisms (Transgenic) Ex. Tomatoes with
flounder gene;
Corn resistant to insects; Bacteria that “eat” oil spills
 Medical breakthroughs – gene therapies
B. DNA Fingerprinting – using gel electrophoresis (p. 405) to make a DNA
fingerprint
Examples:
 To identify organisms Ex. E.coli, salmonella, flu virus that cause illness
 To identify people Ex. Bodies, criminals, innocent prisoners, parents,
children
 To diagnose diseases. Ex. HIV, cystic fibrosis, hemophilia, cancer, flu
 To trace ancestry
There are many examples p. 416-422 of text to give you ideas, but do not use your text to
write the report – just to give you ideas.
Report for Second Quarter:
For each of these TWO technologies (Gene splicing and DNA Fingerprinting)
1. Explain the technique. Be specific and brief (one paragraph) List your source.
2. Research an example of how the technique has been used by humans. You can
use one of the examples listed above or find your own. Be specific in explaining
how the technique was used. Cite your sources – not the textbook. This is the
major part of your report. DO NOT USE INSULIN or INDENTIFYING
CRIMINALS as examples. Find something less common.
3. If this is a controversial example, you MAY give your opinion about its use.
4. #1 should be one paragraph and # 2 should be 3 or 4 paragraphs each. The total
report should be about 3 typed pages.
5. Use “Turn it in”
This report will be due by Jan. 6th, but may be turned in at any time before that date, and
will count as a test grade. The rubric will be on SharpSchool.