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Heat shock pulse T7 polymerase Very few molecules of T7 polymerase are produced by heat shock pulse. Supposedly only one molecule binds to one side of the two partially OVERLAPPING T7 promoters and activate the downstream gene. At least two genes(actually an AD also need to be expressed if combined with the memorizer) are expressed: one repressor for the other side so that only the side hit by the polymerase can fire again, and one T7 polymerase to sustain the gene expression, stabilizing the state of the Randomizer.