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Programmable Cool-Hotter Dry Bath Incubator INSTRUCTION MANUAL _____________________________________________________________________ 1 INDEX Introduction----------------------------------------------------------------------------------------3 Controls and Features----------------------------------------------------------------------------4 Operation Instructions---------------------------------------------------------------------------6 Temperature Calibration-----------------------------------------------------------------------11 Troubleshooting Guide-------------------------------------------------------------------------13 Additional Information-------------------------------------------------------------------------14 Part List-------------------------------------------------------------------------------------------15 Warranty------------------------------------------------------------------------------------------16 Warning : 1. 2. Read and carefully following manual instructions. Do not alter equipment. Failure to adhere to these directions could result in personal and/ or laboratory hazards, as well as invalidate equipment warranty. 2 1. Introduction Overview Programmable COOL-Hoter dry baths use interchangeable block modules for a variety of applications including ligation, storage, restriction digests, denaturing DNA, BUN, melting agar, coagulation studies, in situ hybridization and Hot Start thermocycler reaction. All models incorporate a PI controller for easy temperature selection, rapid heat up and cooling down with excellent stability. Temperature may be set in 0.1 ℃ increments from -10℃ to 100 ℃. The unit contains “Constant Operation” and “Programmable Mode. On “Constant Operation” can set Temperature (from –10 ~ 100℃) and Timer (from 0 ~ 9999 mins). On “Programmable Mode” can set 4 steps and cycle time(from 0 ~ 9 cycle). Each step can set Temperature (from –10 ~ 100℃) and Timer (from 0 ~ 9999 mins). The unit can use RS232 port to do data log. Warnings 1. Use a properly grounded electrical outlet of correct voltage and current handing capacity. 2. Disconnect from power supply before maintenance and servicing. Refer servicing to qualified personnel. 3. In the event solution is accidentally spilled into the bath, disconnect grounded plug and turn incubator upside down to avoid solution contacting the internal components. Remove bottom cover and inspect to assure solution has not contacted elements, thermostat or connector. Replace damaged parts. 4. Do not use in the presence of flammable or combustible material; fire or explosion may result. This device contains components, which may ignite such materials. 5. Refer servicing to qualified personnel. 6. Ensure that the dry bath is connected to electrical service according to local and national electrical codes. Failure to properly connect may create a fire or shock hazard. 7. Always use the supplied handle to remove hot blocks to avoid burning your hand. 3 2. Controls and Features Please refer to figure 1 on the following page for the location of the different keys. PAGE SELECT PAGE START STOP START and STOP Key ENTER Select Next or Last Page Activate and stop operation of the unit ARROW Key (LEFT) Moves cursor to the left ARROW Key (UP) Moves cursor upwards Increase values ARROW Key (RIGHT) Moves cursor to the right ARROW Key (DOWN) Moves cursor downwards Decreases values SET Key Enables the alteration of a selected value Heating Indicate ON Heating Status Red LED light ON to indicate Heating Cooling Indicate ON Cooling Status Green LED light ON indicate Cooling RS 232 Port data Power Switch A Main Power Switch. Press in “I” to switch on the power supply. Press “O” to switch off the power. AC Socket For AC Inlet and fuse Holder I O Connect computer to record 4 INDICATE ON HEATING STATUS ARROW KEY for RIGHT ARROW KEY for UP ARROW KEY for LEFT Heating Cooling PAGE ENTER START STOP COOL-HOTER DRY BATH INCUBATOR INDICATE ON COOLING STATUS SELECTPAGE ARROW KEY for DOWN START& STOP KEY SETKEY Control Pannel AC INLET RS 485 PORT FUSE HOLDER POWER SWITCH I O Rare of the Unit figure 1 5 3. Operation Instructions Constant Operation : 1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away from laboratory traffic. 2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V as indicated on the rating sticker near the AC cord on the back of the unit ). 3. Select a suitable module block and put it into the Dry Bath Incubator. 4. Switch the main power ON. ENTER 5. Use and to select “Constant Operation”. And then press . Consta nt Op era tio n TC ( 0.0) 6. Use and Min 0 to move cursor to which want to set Temperature or Time. ENTER 7. Press and then use and ENTER Finally, press again. START STOP 8. Press the to start heating or cooling. START STOP 9. Press again to stop the unit. 6 to increase or decrease value. Programmable Mode : 1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away from laboratory traffic. 2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V as indicated on the rating sticker near the AC cord on the back of the unit ). 3. Select a suitable module block and put it into the Dry Bath Incubator. 4. Switch the main power ON. ENTER 5. Use and to select “Programmable Mode”. And then press . Prog ra m m a b le Mod e Prog ra m Num b er ( 00) ENTER 6. Press and then then use and to set program file number (It ENTER can maximum save 10 file from 00 ~ 09). Press again. PAGE 7. Press or to next page. Tem p. Step 0 ( 0.0) Step 1 0.0 Step 2 0.0 Step 3 0.0 7 Tim er 0 0 0 0 8. Use ; ; Step; Temperature or Time and to move cursor to which want to set ENTER 9. Press and then use and to increase or decrease value. ENTER Finally, press 10. Press again. to next page. Cyc le Tim e s ( 00) ENTER 11. Press and then use and to increase or decrease value for ENTER cycle times. Finally, press again. START STOP 12. Press the to start heating or cooling. START STOP 13. Press again to stop the unit. 8 Annealing Program : (This program application, please see page 15 for more detail.) 1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away from laboratory traffic. 2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V as indicated on the rating sticker near the AC cord on the back of the unit ). 3. Select a suitable “Annealing Block” and put it into the Dry Bath Incubator. 4. Switch the main power ON. ENTER 5. Use and to select “Annealing Program”. And then press . Anne a ling Prog ra m Initia l Tem p. 95.0 Desc end ing Tem p. 5.0 Hold ing Tim e 5 Fina l Tem p. 4.0 6. Use ; Parameter --- ; and to move cursor to which want to set a. Initial Temp.:Start reaction Temp. b. Descending Temp.:To decrease Temp. on each step c. Holding Time:Holding time for each step d. Final Temp.:Temp. for end step 9 ENTER 7. Press and then use and ENTER Finally, press again. START STOP 8. Press the to start heating or cooling. START STOP 9. Press again to stop the unit. 10 to increase or decrease value. 4. Temperature Calibration Dry Bath Incubator with the optional block has been calibrated as a set. But, the different kinds of block which the ÌT are not the same. For optimum accuracy control temperature or change different kinds of block. Dry Bath Incubator should be calibrated in accordance with the procedure outline below. 1. Insert a 45 mm calibrated laboratory Thermometer into the Thermometer holding port which on the block. ENTER 2. Please switch the main power OFF/ON and press key simultaneously and don’t release them until display over 1100, which on the up left of display. Please see below. And then release them immediately. 1100 Consta nt Op era tion Prog ra m m a b le Mo d e 3. Below page is under Calibration Mode. INNER CALIBRATION TARGETTEMPT. ( 75.0) S: 0.0 R : 28.0 Tim e : 0 Ra te : 959 ENTER 4. Press and then use and to increase or decrease TARGET TEMP value. The target temperature is that you want to accuracy control ENTER temperature value. And then press again. START STOP 5. Press the to start heating or cooling. 6. Until “R” (Real Temperature) reach TARGET TEMP., the timer will be starting to count up. 11 INNER CALIBRATION TARGETTEMPT. ( 75.0) 75.0 S: 0.0 R : 28.0 Tim e : 0 Ra te : 959 7. Until “Time” display 29 mins, you can set “S” value to the same as thermometer. ENTER And then press . INNER CALIBRATION TARGETTEMPT. ( 75.0) 75.0 S : ( 0.0 ) R : 28.0 Tim e : 0 Ra te : 959 8. Please wait for some minutes, that microprocessor will auto adjust temperature until LED display value is the same as thermometer. 12 5. Troubleshooting Guide Many operating problems may be solved by reading and carefully following the instructions in this manual. Some suggestions for troubleshooting are given below. Should these suggestions not resolve the problem, contact the SERVICE DEPARTMENT or your distributor for assistance. If required, please include a full description of the problem. Problem Comments LCD do not light up 1. Check the fues (3A) 2. Ensure that the AC power switch is ON 3. Check the three-pronged power cord are properly plugged into a grounded three-prong AC outlet of the appropriate voltage. 13 6. Additional Information Maintenance The Dry Bath Incubator may be cleaned with a moist cloth containing a mild soap solution. The blocks are aluminum and may be cleaned with any of the commercial aluminum cleaners on the market. Specifications a. Model: MC-01N b. c. d. Microprocessor controller Display: 128 x 64 Dot Matrix Display Operating range: -10℃ to 100 ℃ e. f. g. h. Timer: 0 – 9999 mins Program: Max. 4 steps. Cycles Times: 0 ~ 9 Adjust the temperature: in 0.1 ℃ i. j. Outside Dimension: 20 x 29 x 14 cm (W x D x H) Block Dimension: 125 x 90 x 40 mm (W x D x H) k. Operation Voltages: 110 or 230 voltage 50/60 Hz 14 7. Parts List Item Parts Number Description 1 MC-01-CPU CPU Board 2 MC-01-PB Power Board 3 MC-01-TECPB TEC Power Board 4 MC-01-TC TC Thermocouple K Type 5 MC-01-F Fuse 4A/250V 6 MC-01-NF Noise Filter; Model YL06TI 7 MC-01-PS Power Switch 8 MC-01-MT Main Transformer 9 MC-01-BR Bridge Rectifiers, 20A/600V 10 MC-01-FAN Fan, 110 V 15 8. Warranty MAJOR SCIENCE., warrants apparatus of its manufacture against defects in materials and workmanship, under normal service, for one year from the date of receipt by the purchaser. This warranty excludes damages resulting from shipping, misuse, carelessness, or neglect. MAJOR SCIENCE liability under the warranty is limited to the receipt of reasonable proof by the customer that the defect is embraced within the terms of the warranty. All claims made under this warranty must be presented to MAJOR SCIENCE within one year following the date of delivery of the product to the customer. Major Science Co., Ltd. No. 7, Hwai-Der Street, Pan-Chiao City, Taipei Hsien 220, Taiwan Tel : 886-2-2256-6980 www.major-sci.com.tw Fax : 886-2-2256-6942 E-mail : [email protected] 16