Download BIO 212 SI Kukday--Energetics (2) Review 2/7

Energetics (2)
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Caleb I.
BIOL 212
Dr. Kukday
2/07/2017
1.) Vocabulary Review- Match the Terms & Definitions:
a. Binding to the enzyme at a spot
i.
Activators ______
different from the active site to
ii.
Inhibitors ______
control activity
iii.
Krebs Cycle ______
b. Use positive allosteric
iv.
Aerobic Respiration ______
regulation to enable enzymatic
v.
Allosteric Regulation ______
activity
vi.
Electron Transport Chain ___
c.
O2
vii.
The final electron acceptor__
d. Use negative feedback to
viii. Anaerobic Respiration ____
slow/stop enzymatic activity
ix.
Feedback Inhibition ______
e. Respiration done in the presence
x.
Irreversible inhibition______
of oxygen
xi.
Glycolysis ______
f. Blocking an enzyme as a result
of feedback
g. The process that turns acetyl
CoA into NADH and FADH2
h. This process happens outside
the mitochondria and turns
glucose into pyruvate
i. Inhibition that requires making
a new enzyme to resume the
process
j. Process where most of the ATP
is actually produced in the
mitochondria
k. Creating ATP in the absence of
oxygen
2.) Can you predict the consequences of mutations in an enzyme that is part of a metabolic
pathway? ----- Predict what would happen if the enzyme between pyruvate and acetyl
CoA were inhibited. Predict what would happen if the final product of the TCA cycle
inhibited the enzyme that turns pyruvate into lactate:
1060 Hixson-Lied Student Success Center  515-294-6624  [email protected]  http://www.si.iastate.edu
3.) Can you identify types of enzyme regulation (emphasis on feedback inhibition)? ----Label the reaction types below as either “normal reaction”, “competitive inhibition” or
“noncompetitive inhibition”. Also label the active site, the allosteric site and the
substrate:
4.) Can you compare aerobic and anaerobic respiration pathways with respect to differences
in products and energy intermediates produced? ----- Draw the overall products and
reactants of cellular respiration. Balance the equation accordingly. After that, fill in the
blanks on this chart with the following options:
Glycolysis, Citric Acid Cycle, Electron Transport Chain, O2, H2O, Cytoplasm, Mitochondrial
Matrix, and Inner membrane
1060 Hixson-Lied Student Success Center  515-294-6624  [email protected]  http://www.si.iastate.edu
5.) Can you identify the role of energy rich intermediates and electron acceptors at different
stages in respiration (glycolysis, Krebs cycle, Ox Phos)? ----- Finish the oxidation of
these energy rich intermediates. Be sure to include the electrons and energy gain/release:
i.
NADH 
ii.
FADH2 
iii.
ATP 
iv.
GTP 
6.) Can you illustrate the steps that lead to the ultimate production of ATP at the inner
mitochondrial membrane? ----- Label the missing parts of the figure below. Find where
the proton gradient is created and where the gradient is used to turn ADP into ATP:
1060 Hixson-Lied Student Success Center  515-294-6624  [email protected]  http://www.si.iastate.edu
Dr. Kukday’s “Can You?” Questions (Highlighted ones are addressed in this worksheet, you will
need to address the ones that aren’t highlighted on your own time):
Paralysis Case:
1.) Can you identify types of enzyme regulation (emphasis on feedback inhibition)?
2.) Can you predict the consequences of mutations in an enzyme that is part of a metabolic
pathway?
3.) Can you compare aerobic and anaerobic respiration pathways with respect to differences
in products and energy intermediates produced?
4.) Can you identify the role of energy rich intermediates and electron acceptors at different
stages in respiration (glycolysis, Krebs cycle, Ox Phos)?
5.) Can you identify treatment options for PDCD based on knowledge of the reactions
catalyzed by PDH and the aerobic respiration pathway as a whole?
DNP Case:
1.) Can you illustrate the steps that lead to the ultimate production of ATP at the inner
mitochondrial membrane?
2.) Can you identify or predict the effect of inhibitors of Ox Phos?
3.) Can you differentiate between the mechanism of action of these inhibitors of Ox Phos?
(discussed in next class)
4.) Explain the connection between the effect of inhibition of Ox Phos with the symptoms
that a person who consumes the inhibitors will experience?
1060 Hixson-Lied Student Success Center  515-294-6624  [email protected]  http://www.si.iastate.edu