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Transcript
Topic 3 & 10: Genetics
3.5 Genetic Modification and Biotechnology
Understandings
3.5.1
3.5.2
3.5.3
Gel Electrophoresis is used to separate proteins or
fragments of DNA according to size.
PCR can be used to amplify small amounts of DNA.
DNA profiling involves comparison of DNA.
Applications and Skills
Use of DNA profiling in paternity and forensic
investigations.
Analysis of examples of DNA profiles.
Gene transfer to bacteria using plasmids makes use
of restriction endonucleases and DNA ligase.
Assessment of the potential risks and benefits
associated with genetic modification of crops.
Analysis of data on risks to monarch butterflies of
Bt crops.
3.5.4
Genetic modification is carried out be gene
transfer between species.
3.5.5
Clones are groups of genetically identical
organisms, derived from a single original parent
cell.
Many plant species and some animal species have Design of an experiment to assess one factor
natural methods of cloning.
affecting the rooting of stem-cuttings.
Animals can be cloned at the embryo stage by
breaking up the embryo into more than one group
of cells.
Methods have been developed for cloning adult
Production of cloned embryos produced by
animals using differentiated cells.
somatic-cell nuclear transfer.
Cambridge University Press – Biology for the IB Diploma
3.5 – pages 124-133
3.5.6
3.5.7
3.5.8