Download PCR Study Questions

Study Questions for Molecular Diagnostics Section
1. What is DNA?
2. Describe the structure of DNA. You may use words or graphics.
3. DNA strands can come apart and go back together. Why is this important?
4. What is meant by the ‘melting’ or ‘denaturation’ of DNA?
5. Which characteristic of DNA’s structure contributes most to the ‘melting point’
determination?
6. What is meant by the terms hybridize, anneal or renature when referring to DNA?
7. What is the correct sequence relative to DNA replication (steps of PCR)?
8. PCR is an abbreviation for ________________?
9. Name and describe the functions of the three work areas used for PCR.
10. PCR can be used for amplifying sequences of which nucleic acids?
11. What is the additional step required to perform PCR if the target nucleic acid is RNA?
12. Compare and contrast the nucleic acids DNA and RNA?
13. What is the purpose of the internal control (name two)?
14. List the six (6) components of Master Mix and the functions of the components.
15. What four (4) factors must be considered in designing a probe?
16. Describe the clinical significance of the following PCR tests:
a. CMV
b. HSV 1
c. HSV 2
d. EBV
e. Chlamydia
f. Gonorrhea
g. HCV
h. RVP
17. Why is uracil N-glycosylase (UNG) so important in our master mix?
18. Describe the difference between a primer and a probe.