Download P006 Could Stem Cells be a source for primary cells in HTS scenario?

P006 Could Stem Cells be a source for primary cells in HTS
scenario?
Ródenas C. 1, Cruz ME.1, Del Real G.1, Alamillo A.1,
Carranza C.1, Gonzalez del Río R.2, Mendoza A. 2,
Issandou M. 3, De la Fuente J. 1
1
Molecular Discovery Research, S&CP, GlaxoSmithKline,
Tres Cantos
2
Diseases of the Developing World Medicines Discovery
Centre, GlaxoSmithKline, Tres Cantos
3
Drug Discovery, DPU Lipid Metabolism, GlaxoSmithKline,
Les Ulis
One of the main goals for the Pharmaceutical Industry is to
reduce the high attrition rate in the final phases of the Drug
Discovery process. The efficiency of this process will be highly
improved if we can reveal the negative characteristic of the
compounds in the early phases, as the HTS. This issue could be
tackled by developing more in vivo-like assays.
The Primary cells are extremely difficult in culture and have a
limited expansion potential. The use of stem cells is one of the
strategies to create better cell models closer to the cells in human
target tissues and more predictive for efficacy and safety in vivo
than the stable cell lines currently used.
The adult stem cells could be the key as they can expand to high
number of cells and differentiate into any adult specialized cell
type. At the same time, the use of adult stem cells overcomes
any ethical consideration. But because the telomeres shortening
restrict their ability to proliferate we carried out an immortalization
process to increase their expansion potential.
We immortalized adult mesenchymal stromal cells from adipose
tissue (ADSCells) and most of the obtained clones are able to
differentiate into different types of primary cells and it is easy to
scale them up for uHTS requirements.