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BIOLOGY TEACHING LABORATORY PRACTICAL RISK ASSESSMENT FORM Module Number & Organiser 304 James Chong Brief Description of Work Microbiology practical to test the effectiveness of antimicrobials. Work will involve growing bacteria on solid media. Location of Activity Biology Teaching Labs Assessed by Dr Adrian Harrison Name Date Reviewed ………………………… Signature 27/04/07 Date 1. Identification of Significant Hazards What are the Significant Hazards? Biological: work with the Hazard Group 2 organism Staph. aureus which could cause infection Fire: work with Bunsen burners for aseptic techniques could cause fire Slip: Spillage of non-hazardous material could present a slip hazard Trip & Collision: large number of students and bags in laboratory area Electrical: use of equipment 2. Individuals who Might be Harmed by Hazards Who Might be Exposed to Hazards? Undergraduate students taking part in the practical, demonstrators and academics supervising the practical, Teaching Laboratory staff preparing and providing the materials and reagents. 3. Measures Required to Control the Risks Please consider risk reduction methods for each of the hazards identified above. Can any of the identified hazards be eliminated? Is there a safer alternative? Hazards Measures Required to Control Risk Staph. aureus and other microbes Good microbiology practice and aseptic technique will be applied to control possible exposure to infectious agents e.g. as a result of aerosol generation. Strains used are either ACDP Hazard Group 1 organisms, or a Staph aureus that has a known sensitivity to antibiotics. All cultures should be treated as potentially pathogenic. Fire (from Bunsen Burners) Students are advised of this hazard and encouraged to use good laboratory practice. A VLE video is available for students to review good practice. Residual Level of Risk MEDIUM LOW Slip (from spillages) Trip and collision (from students) Electrical LOW Application of Good Laboratory Practice LOW Large numbers of students mean that collisions are possible. Students are advised to store bags safely to remove trip hazards. Spectrophotometers are electrically tested LOW 4. Additional Information for Technical Staff. Identify any specific precautions that need to be taken during preparation of material for the practical Care when handling molten agar required for plate pouring. Standard ACDP containment conditions and working practices for Hazard Group 2 micro organisms. Microbial waste to be autoclaved before final disposal and benching disinfected after use.