Download Steps to drawing Epithelial Histology diagrams. 1. Go to http://bit.ly

Steps to drawing Epithelial Histology diagrams.
1. Go to http://bit.ly/EpiHist1 or http://histology-umms.org/node/82.html
2. Scroll down to Slide 153 -- ________________________, H&E, 20X (stratified
_______________________ epithelium, nonkeratinized).
3. Click on WebScope to the right.
4. You are now looking at the esophagus-________________________________________. There is fat, blood vessels, etc. Where
specifically do you think that the Epithelium will be? Look at your notes if necessary.
Structures you should be able to identify:

_______________________________________

________________________________________

Location and Shape of _____________________
5. Now go back to http://bit.ly/EpiHist1 or
http://histology-umms.org/node/82.html
6. Scroll down to the heading "Epithelial Tissue" and scroll down to slide 210 -________________, H&E, 20X (simple _______________ epithelium)
7. On the right, click on "WebScope"
8. This is the ____________________ and it has tubes or tubules which filter (in and out)
different ions and fluids. Eventually the filtered material becomes urine and goes
through the ureters to the bladder and out of the body. What structures do you think
you should be able to see?
9. Repeat the same procedure for slide 176 -- ________________, H&E, 20X
(simple __________________ epithelium).
For this slide the large vacuole/bubble shaped structures are called ___________ cells.
These produce mucous--a slippery secretion that covers mucous membranes. For
instance, your stomach produces mucous which keeps the acid inside the stomach from
affecting the lining or epithelium of the stomach. The average human body produces 1
liter of mucus per day. (Source: Wikipedia).
Hematoxylin and eosin stain (H&E stain or HE stain) is one of the principal stains in
histology. It is the most widely used stain in medical diagnosis and is often the gold
standard; for example when a pathologist looks at a biopsy of a suspected cancer, the
histological section is likely to be stained with H&E and termed "H&E section", "H+E
section", or "HE section".
The staining method involves application of hemalum, a complex formed from aluminum
ions and hematein (an oxidation product of haematoxylin). Hemalum colors nuclei of
cells (and a few other objects, such as keratohyalin granules and calcified material)
blue. The nuclear staining is followed by counterstaining with an aqueous or alcoholic
solution of eosin Y, which colors eosinophilic structures in various shades of red, pink
and orange. A mnemonic often used to remember the coloring process is Acidic
Attaches Acidophilic Applying Auburn and Basic Binds Basophilic Becoming Blue.
(Source: Wikipedia).