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Genetics of new sources of rust resistance, backcrossing in wheat & overseas testing for stem rust Harbans Bariana Genetics – APR to stem rust Burrion (Sr31+APR)/LB - Two APR genes in Kenya AWCC993/Yitpi - Monogenic segregation AWCC1141/Yitpi - Monogenic segregation 2 Genetics – APR to leaf rust Genetic analysis of two landraces showed digenic segregation Monogenically segregating populations (MSPs) were isolated Five MSPs are confirmed and genomic location studies are in progress Two gene segregation was noted in Sonora/BT Schomburgk (BTS) population. One slow rusting minor gene is contributed by Sonora and a major gene is contributed by BTS Monogenic segregation for an APR gene from Westonia was noted in a cross made for stripe rust resistance analysis 3 Genetics –APR to stripe rust Trigenic segregation in Sonora/BT Schomburgk (BTS) population. Sonora appear to contribute one race-specific and one APR gene. One APR is contributed by BTS BC1F2 analysis of Wollaroi/2*NYB3 cross confirmed segregation of 3 to 4 genes for adult plant stripe rust resistance 4 Genetics –Seedling resistance stripe rust A new widely effective gene for stripe rust resistance was shown to be linked closely in repulsion with Yr4 Genetic analysis based on Frelon/NYB3 F3 population demonstrated the presence of one more gene in addition to Yr17 5 Genetics – Durum wheat Wollaroi showed infection type 23n to 3n in the seedling stage. This phenotype segregated with Qyr.sun-2A which explains 9 to 12% phenotypic variation. Request to name this locus has been sent to Bob McIntosh. It is likely to be named Yr56. Identified new sources of resistance against durum leaf rust pathogen, populations are being developed. An F5 population of a durum genotype carrying APR to stem rust will be phenotypes in 2013 Tamaroi/Bansi F6 population showed monogenic segregation for leaf rust resistance against wheat leaf rust pathogen 6 Triple rust resistant donor development through backcrossing Yr40/Lr57 – Five backgrounds Lr49,Lr13, Lr34 - four backgrounds Yr47/Lr52 – Four backgrounds Yr35/Lr53 – Five backgrounds Lr67/Yr46/Sr55 – Three backgrounds Yr49 – Initial crosses made in four backgrounds Yr37/Lr54 – Initial crosses made 7 Amelioration of donors Sr50 - it was highly susceptible to stripe rust and has been crossed with Yr40/Lr57 and Yr47/Lr52 Yr49 - It was highly susceptible to leaf rust and stem rust. It has been crossed with lines leaf rust and stem rust resistant donors Yr34 - It is also being improved for leaf rust and stem rust resistance Sr39 (shortened segment ) has possibly lost leaf rust resistance and it was crossed to top up leaf rust resistance 8 Ug99+Sr24 Screening Bullet, Cadoux, Condor , Clearfield JNZ, Dakota , Gabo, Gamenya, GBA Sapphire, Janz, Kidman, Meering, Mitre, Petrie,, Waagan, – Sr28 type resistance , Bolac, Crusader, Carinya , EGA Wentworth - Other than Sr28 9 Potential threats › Durum leaf rust races › Ethiopian and Indian stem rust races - (Sr13 virulence) 10 Response to American leaf rust pathotypes Bellaroi Caparoi Gundaroi Hyperno Jandaroi Saintly Kalka Tjilkuri WID802 WID803 Wollaroi Yallaroi Tamaroi Kamilaroi CA 1.2 3+ 3+ 3+ 3+ 3+ 3+ 3+ 3+ 3+ 3+ 3+ 33+ 3- 09 AZ 103 A ; ; ; ; 3; ; ; ; 3+ ;23+ 3+ ;1- THBJ ; ; ; ;1; ; ; ; 3+ ; 3;1; 11 Kenya - Ug99+Sr24 Genotypes 14 9 5 1 3 14 B04-17 Bellaroi Caparoi Hyperno Jandaroi Kalka Saintly Tamaroi Tjilkuri Wollaroi Rust response 2 3 4 5 6 seg 65 67 5,3,2 2,45 34 23,5 3 5,3 23,4 5 12 Ethiopia - Sr13 virulence Genotypes 2 16 29 7 5 Arrivato Bellaroi Caparoi Gundaroi Hyperno Jandaroi Kalka Kamilaroi Saintly Tamaroi Tjilkuri WID802 WID803 Wollaroi Yallaroi Rust response 5 6 7 8 seg 5 TO 6 67 67 7 6 5 67 7,5 6 7 67 67 56 5E, 78L 7 13 Summary Focus is to characterize APR genes other than known Facilitate release of triple rust resistant varieties for growers through provision of resistance donors National and international training 14 Thank You IARI RRS, Wellington, India KARI, Njoro, Kenya Ug99+Sr24 40A – Sr24 PBI, Cobbitty, Australia 98-1,2,3,5,6,.7+34-1,2,7+Sr38 15