Download Non-coding RNAs are widely distributed in the 3 life domains

RNOMICS
J. H. Leitão
March 2017
Non-coding RNAs are widely distributed in the 3 life domains
ncRNA in Eukaryotes and RNAi
Potential applications of miRNA in plants
Bacterial sRNAs
sRNA encoding Region
Intergenig Region
Lacks obvious start and stop codons
ROLE OF sRNAs IN BIOFILM FORMATION
Trends Microbiol. 2013 Jan;21(1):39-49.
CsrA and CsrA homolog RsmA and effect of sequestration by sRNA(A) Gene
expression is controlled by CsrA binding to leader segments of target mRNAs
(e.g. pgaABCD involved in PGA biosynthesis and export) affecting their
translation and stability. CsrA activity is repressed via sequestration of CsrA by
sRNAs CsrB/C, thereby inhibiting CsrA regulatory activity. (B) CsrA activity is
enhanced by CsrD as well as the sRNA McaS, a component of the CsgD network
which represses CsgD. Repression by CsrA on pga mRNA translation is negated
by NhaR, an activator of PGA biosynthesis. (C) Schematic overview of the
signaling cascade that converges on the sRNAs RsmY and RsmZ, which act by
sequestering the translational repressor RsmA. RsmA reciprocally regulates
factors involved in the planktonic/sessile switch indicated by activation of genes
involved in motility or by repressing genes required for biofilm formation (Pel,
Psl polysaccharides). Dashed lines indicate that the connection has not been
fully demonstrated or is not understood at the molecular level.
Trends Microbiol. 2013 Jan;21(1):39-49.
sRNAs from bacteria modes of action
Identification and characterization of ncRNAs
The miRNA database
The Rfam database
The Vienna package webserver
Identification of Hfq-like proteins from bacteria of the Burkholderia cepacia complex
The strategy