Download Daniel Kaganovich Molecular Mechanism of

Laboratory Profile
Principal Investigator:
Daniel Kaganovich
Molecular Mechanism of Neurodegenrative Disease and Protein Aggregation
Location: Silberman Wing 3, 5th. floor Room 3-536
Communication:
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E.Mail: [email protected]
Phone: 054-246-2875
Web: www.kaganovichlab.com
Brief Description of Research:
For a cell to be alive, it must coordinate the
function of thousands of proteins and macromolecules over space and time. This
coordinated function of proteins relies on efficient management of protein folding
throughout the cell. Our lab uses high-resolution 3D time-lapse imaging to study
the way in which cells orchestrate the function of a vast and dynamic protein
folding quality control system, which includes chaperones that enhance protein
folding and regulate protein aggregation. From basic findings in simple cellular
models, we develop animal models of neural function and neurodegenerative
disease. Our goal is to understand some of the ways in which neurons manage
protein folding differently form other cells, and how this contributes to the
pathology of diseases such as ALS, Alzheimer’s, Parkinson’s, and Hundington’s
Diseases. (for more details, please see website).
Techniques used include: high-resolution 3D live-cell confocal imaging; live cell
imaging in living organisms; high-throughput image-based genetic screening of
yeast genome libraries; super-resolution microscopy imaging in live cells; TIRF
microscopy; molecular biology and biochemistry; yeast genetics; mammalian cell
culture; computational approaches to understanding protein misfolding and
aggregation.
Proposed Research Projects:
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Topic 1: Discovery of novel regulators of protein aggregation using yeast image-based
genetics screening and live-cell imaging.
Topic 2: Visualizing neural activity in live C. elegans worms and neurodegenerative
disease models.