Download Phospho-specific antibodies Cat. No. A060

Davids Biotechnologie GmbH
Röntgenstraße 3
93055 Regensburg Germany
www.davids-bio.de [email protected]
++49 (0)941 948228
Phospho-specific antibodies Cat. No. A060
 Package
 Preimmune serum
 Aliquot of the raw serum as backup
 Affinity purified anti-phospho antibodies (non-phosphopeptide depleted)
 Phosphopeptide
 Non-phosphopeptide
 Operation Instruction
Peptide
Synthesis
&
Immunizations (1-3)
Synthesis of the phospho- and
the non-phosphopeptide. The
phosphopeptide is conjugated to
a carrier for immunizations.
 raw sera contains antiphosphoand
anti-nonphosphopeptides
Affinity purification (4)
We immobilize the phosphopeptide to a matrix and purify the
sera to receive all antibodies
recognizing the peptide.

antibodies
recognizing
phospho- and non-phosphopeptide
Depletion of antibodies recognizing non-phosphopeptides (5)
To get rid of the antibodies recognizing the non-phosphopeptide we make subsequent affinity
purification with the non-phosphopeptide as matrix.
 antibodies recognizing the phosphopeptide (final product)