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Supplemental Figure 3.
Spurious amplification of SCR1 from genomic DNA of
representative A. thaliana acccessions. Equivalent amounts of genomic DNA isolated
from different accessions were subjected to PCR using the same pair of SCR1 primers
(the PseSCR3 and PseSCR5 primers described by Shimizu et al. 2004). Note that DNA
from the C24 and Mt-0 accessions, which lack SCR1 (Table 1 and Figure 3), produce
faint amplification products (asterisk), presumably due to annealing of the primers to
non-SCR1 sequences. We were unable to sequence these spurious PCR products
directly, probably because they consist of a heterogeneous mix of sequences. This
conclusion was confirmed by sequencing individual clones obtained by TA cloning of the
spurious C24 PCR products in the pGEMT plasmid, which returned sequences
corresponding to various genomic regions.