Download Friday Review Session Questions 1

Friday Review Session Questions
1- What is the difference b/w Mitosis Promoting Factor (MPF) and checkpoints at M1 phase?
MPF allows the cell to go from G2 to M when cyclin and CDK concentrations are adequate, this regulates
the cell cycle. Mitosis checkpoints makes sure division occurred correctly and chromosomes divided
evenly and regulates the cell cycle.
2- “Guilty Bob” is accused of murder. You take a saliva sample (for DNA like in the PCR 1 lab). (A) Will the
normal bacteria in Bob’s mouth contribute DNA to the sample? (B) Will bacterial DNA cause Bob to get
away with murder? Why or why not?
(A): Yes, all DNA in the sample is extracted
(B): No, primers in PCR are specific to the gene in question.
3- How can so many unique antibodies be produced?
VDJ recombination (need more than just buzz words for a full credit answer), discuss the mechanism
and location.
4- What happens if B cells and T cells cannot recognize self-cells?
5- How do duplicated genes become functional and does mutation make it both functional and/or nonfunctional?
6- How can you tell if a newly discovered gene has been acquired through lateral gene transfer (LGT)?
Unanswered Questions
What is the significance of having unique primers?
How is the sequence “put back together” in pyrosequencing?
What happens to the organelles within a B cell after activation?