Download Nucleotide Sequence of Rainbow Trout a

Rapid Communication: Nucleotide Sequence of
Rainbow Trout a-Globin I and IV cDNA
Goro Yoshizaki, Atsuko Takano, Takashi Aoki, and Fumio Takashima
Department of Aquatic Biosciences, Tokyo University of Fisheries,
Konan, Minato-ku, Tokyo 108, Japan
Name of Sequence. Rainbow trout a-globin I and IV
cDNA.
EMBL/GenBank Accession Number. a-globin I,
D88113; a-globin IV, D88114.
Species. Oncorhynchus mykiss.
Origin of Clone. Messenger RNA was isolated from
total blood cells. Complementary DNA was synthesized using the cDNA Synthesis Kit (Pharmacia
Biotech, Uppsala, Sweden). A library was then
constructed by cloning cDNA into pUC118. The library
was screened with carp a-globin cDNA (Takeshita et
al., 1984). Two positive clones (clone 1 and 5 ) were
identified and sequenced with the dideoxy method.
Comparison with Related Sequence. The deduced
amino acid sequence of clone 1 showed 97.9% identity
to rainbow trout a-globin IV (Bossa et al., 1983).
Clone 5 showed 97.9% identity to rainbow trout aglobin I (Petruzzelli et al., 1989). Clones 1 and 5
demonstrated 61.3% amino acid identity with one
another. Also, the amino acid identities of clone 1 and
5 to human a-globin (Kleinschmidt and Sgouros,
1987) were 50.4% and 56.7%, respectively.
Sequence Data. The nucleotide and deduced amino
acid sequence of a-globin I and IV are shown in
Figures 1A and B, respectively. These clones had an
open reading frame that encoded 143 amino acids.
Comments. The identity data indicate that clones 1
and 5 encode a-globin IV and I, respectively. However,
the deduced amino acid sequence of clone 5 was two
residues longer than that of a-globin I. The distal and
proximal histidines (Figure 1; double underline) were
identified, and the predicted heme contact region
(Figure 1; underline) was found to be well conserved
(i.e., approximately 85% identity) between the rainbow trout and human sequences.
(Salmo irideus). Biochim. Biophys. Acta 995:255.
Takeshita, S., T. Aoki, Y. Fukumaki, and Y. Takagi. 1984. Cloning
and sequence analysis of a cDNA for the a-globin mRNA of
carp, Cyprinus carpio. Biochim. Biophys. Acta 783:265.
Key Words: Rainbow Trout, Complementary DNA,
Nucleotide Sequences, Cloning, Amino Acid Sequences
J. Anim. Sci. 1997. 75:1426
Literature Cited
Bossa, F., D. Barra, R. Petruzzelli, F. Martini, and M. Brunori. 1983.
Primary structure of hemoglobin from trout ( Salmo irideus) .
Amino acid sequence of a-chain of trout Hb I. Biochim. Biophys. Acta 536:298.
Kleinschmidt, T., and J. G. Sgouros. 1987. Hemoglobin sequences.
Biol. Chem. Hoppe-Seyler 368:579.
Petruzzelli, R., D. Barra, L. Sensi, F. Bossa, and M. Brunori. 1989.
Amino acid sequence of a-chain of hemoglobin IV from trout
Received October 7, 1996.
Accepted January 16, 1997.
Figure 1. Nucleotide and deduced amino acid sequences of rainbow trout a-globin I (A) and IV (B).
Nucleotide number and the amino acid residue number
are shown at right. Distal (amino acid number 60) and
proximal histidines (amino acid number 89) are doubleunderlined and the heme-contact regions are underlined.
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