Download Signal Amplification by the Generation of Protein Polymer Networks

Signal Amplification by the Generation of Protein Polymer
Networks
Bo-Shiuan Lin, James R. Carey*
Traditional bacterial detecting technologies, such as the plate count technique, enzyme-linked
immunosorbent assay1 (ELISA), biochemical tests, and the polymerase chain reaction (PCR) are
time-consuming and labor-intensive. The protein conglomeration based amplification method (PCBAM)
might change this situation. The core of this developing method as shown in figure 1 is utilize biotinyled
antibodies labeled the tetravalent streptavidin2 (SA-DA), 2° antigen and 2° antibody to forming
immunocomplexes. The assumption is that the complexes would through chemical, biological or physical
reaction to achieve continuous signal amplification cause protein polymer networks and can be visually
detected in the end.
Now we can confirm that using the SA-DA enables low concentration from 6.25×10-3 pg/mL to 6.25×10-8
pg/mL of antigen can enhance the absorption, and have a regular increase in absorption with the increasing
concentration. The maximum of coefficient of variation is 9.95%. However, streptavidin would nonspecific
binding to the well cause the background has rather high absorption, and we still need to use the enzyme
immunoassay analyzer to detect the absorption.
Fig. 1
(1). Engvall E, Perlmann P., Immunochemistry, 1971, 8, 871-874.
(2). González, M., L. A. Bagatolli, I. Echabe, J. L. R. Arrondo, C. E. Argaraña, C. R. Cantor, and G. D.
Fidelio., J. Biol. Chem., 1997, 272, 11288-11294.