Download basic characterisation of hiPSC.

Basic characterization of hiPSC
BSCC will provide a basic characterization of hiPSC including:
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Maintenance in culture as established line for 10 passages. Only clones
surviving, as typical pluripotent stem cell colonies for an extended period
are likely to be true stem cells
Human ES cell surface antigen profile
Expression of key endogenous pluripotency-associated genes
Evidence of transgene silencing
NOTE: In addition to the basic characterization, several other analyses are likely
to be needed before the cells can be used for experiments aiming at tissuespecific disease modelling. BSCC can help with these according to agreement
with the client. These include for example:
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Authentication of cell line identity by DNA fingerprinting.
Karyotype analysis, either by G-banding (Medix) or by bead hybridization
(Karyo-Lite, Turku center for Biotechnology)
Pluripotent differentiation potential in vitro (in embryoid bodies) or in
vivo (in mouse teratomas)
Targeted differentiation of pluripotent stem cells into definitive
endoderm progenitors