Download Mitochondrial Function, Cellular Energy Flux and Hypoxia Analysis

Mitochondrial Function, Cellular Energy Flux and Hypoxia Analysis
on a Fluorescence Plate Reader
LUXCEL’s cell biology reagents offer a simple and convenient fluorescence-based, 96-well or 384-well approach to
®
the direct real-time analysis of Mitochondrial Respiration (MitoXpress Xtra – Oxygen Consumption Assay), Glycolysis
(pH-Xtra
™
- Glycolysis Assay) and Intracellular Oxygen Concentration (MitoXpress
®
Intra – Intracellular Oxygen
Assay), for use with a standard fluorescence or TR-F plate reader.
MitoXpress® Xtra – Oxygen Consumption Assay
(HS method)
38
 Whole cells – adherent or suspension
34
Lifetime (s)
36
 Isolated mitochondria and permeabilised cells
 3D cultures: tissues, spheroids, scaffolds & matrixes
 Isolated enzymes, bacteria and yeasts
32
Untreated
30
1uM Antimycin
3.3uM FCCP
28
26
24
Oxygen consumption is the most important parameter for the direct
0
and specific assessment of the function of the ETC, the cornerstone
of
oxidative
phosphorylation
and
cellular
metabolism,
50
100
150
Time (min)
and
mitochondrial function.
Easy “mix and measure” protocols, compatible with a range of commercial assays for mitochondrial membrane
potential, reactive oxygen species (ROS) production and cellular ATP levels. Routinely configured as a screen for
drug-induced mitochondrial toxicity.
600
™
pH-Xtra - Glycolysis Assay
 Convenient Extracellular Acidification assessment (ECA)
 Cell based Glycolytic Flux

% Effect
500
400
ATP
Oxygen
300
pH
also pH in biological and environmental samples
200
 Whole cells and 3D cultures
100
0
Rotenone
Antimycin
FCCP
Oxamic Acid
UT
Untreated
Cellular Energy Flux
Multiparametric (or multiplex) combination of MitoXpress
®
Xtra – Oxygen Consumption Assay (HS method) with
™
Luxcel’s pH-Xtra - Glycolysis Assay allows the simultaneous real-time measurement of mitochondrial respiration and
glycolysis and analysis of the metabolic phenotype of cells and the shift (flux) between the two pathways under
pathological states.