Download Organic Compounds Lab

Organic Compounds Lab
Procedure
General Directions for all Tests
Before beginning, make predictions for all
tests.
2. Label the test tubes 1-7
Note do not cross contaminate tubes between
tests. Use the same substance in the same
tube for each test
3. Place a small amount of each substance in the
tube (approx. 1 cm)
4. Add 8-10 drops of each indicator to the tube
and record your results.
1.
Lipid Test – Sudan III Stain
This indicator will turn bright red or pink in
the presence of lipids.
Protein Test
Biuret reagent will turn from blue to violet
in the presence of proteins.
*Caution* this indicator is corrosive and
will burn your skin!
Iodine Complex Carbohydrate
Test
Complex carbohydrates or
polysaccharides are tested for using
Iodine. It will turn from a light brown to a
dark blue or black.
Simple Sugar Test
Monosaccharides and disaccharides are
indicated when the sample treated with
Benedicts solution is heated. If simple
sugars are present it will turn from blue to
green, yellow, orange, or red.
pH test.
Apply a small amount of each substance
to pH paper and compare to the provided
color chart to determine pH.