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Mini Lab Respiration Experiment
Name ______________________
It is possible to demonstrate that plants respire by testing for the presence of carbon dioxide. Bromothymol
blue is blue in an alkaline environment, but turn yellow in an acid environment. Carbon dioxide in the
presence of water forms a weak acid.
Fill three test tubes to within 3 cm of the top with the bromothymol blue solution or with cabbage juice
indicator. Add a 10 cm sprig of Anacharis (Elodea) to two of the test tubes.
Place one test tube with a plant and one without a plant in the dark. Put the other test tube in bright sunlight
(by the window). Observe the tubes the next day. Answer questions below on day two and complete the
data table above on day two.
Why did you leave one test tube without anacharis?
Which of the three tubes showed a change in color?
Using information gained from this experiment, explain the relationship between photosynthesis and
respiration in the anacharis. Use specific examples from the qualitative data you collected in the experiment
to justify your explanation.