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Patient’s weight (kg) <5 5-7 7-15 16-20 20+ Recommended Volume (mL) of Blood Aerobic 1 3 4 8 10 Recommended volume (mL) of blood if anaerobic culture added Aerobic Anaerobic 0.5 0.5 1.5 1.5 3 1 5 3 5 5 If total blood obtained for blood culture is < 4 ml, use a pediatric blood culture bottle If total blood obtained for blood culture is > 4 ml use adult blood culture bottles If an anaerobic culture is desired place recommended volume of blood (up to 5 mls) into the aerobic bottle and the remaining into the anaerobic bottle. In general, the more blood that can be obtained, the better the potential yield on that blood culture. *There may be clinical situations where the volume of blood that is obtained for sampling will differ from recommendations according to patient condition and diagnosis. If an LIP wants a different volume than the one listed, he or she should discuss that with the practitioner drawing the blood. Baron, E., Miller, J., & Weinstein, M. (2013). A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the infectious diseases society of America (IDSA) and the American society for microbiology (ASM). Clinical Infectious Diseases, 54(4), e22-e121. Garcia, R., Spitzer, E., Beaudry, J., Beck., Diblasi, R., Gilleeny-Blabac, M., . . . Torresgrosa, E. (2015). Multidisciplinary team review of best practices for collection and handling of blood cultures to determine effective interventions for increasing the yield of true-positive bacteremias, reducing contamination, and eliminating false-positive central line associated bloodstream infections. Infection Control and Hospital Epidemiology, 43, 1222-1237. Gaur, A., & Huskins, W. (2015). Guidance for collection of blood cultures from children & adolescents with a central line. ().Children's Hospital Association. Mayo Medical Laboratories. (2015). Recommended pediatric weight-based blood culture volumes. Retrieved from http://a1.mayomedicallaboratories.com/webjc/attachments/128/2d2d720recommended-pediatric-weight-based-blood-culture-volumes.pdf Lancaster DP, Friedman DF, Chiotos K, Sullivan KV. Blood Volume Required for Detection of Low Levels and Ultralow Levels of Organisms Responsible for Neonatal Bacteremia by Use of Bactec Peds Plus/F, Plus Aerobic/F Medium, and the BD Bactec FX System: an In Vitro Study. J Clin Microbiol 2015;53:3609-3613. Schelonka RL, Chai MK, Yoder BA, Hensley D, Brockett RM, Ascher DP. Volume of blood required to detect common neonatal pathogens. J Pediatr 1996;129:275-278. Yaacobi, N., Bar-Meir, M., Shchors, I., & Bromiker, R. (2015). A prospective controlled trial of the optimal volume for neonatal blood cultures. The Pediatric Infectious Disease Journal, 34(4), 351-354.