Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download ALL: What is diffusion? What are the consequences to molecules in
Document related concepts
Transcript
Translation materials ALL: What is diffusion? What are the consequences to molecules in the cell? How do they get ‘where they are going’? tRNA How does a tRNA 'find' it's amino acid? How does a tRNA find & recognize its codon? How does it know when to give its amino acid to the growing protein? mRNA Where does it come from? What does a codon look like in an mRNA? What is the instruction to start making protein? What is the instruction to stop? synthetase How does it recognize the amino acid it works with? How does it recognize the tRNA it works with? ***if you don't know, just consider the information available to the molecule itself--how COULD it 'know'? ribosome How do the large & small subunits get together with the mRNA? How does it know where to start? How does it 'define' a codon? How does it know when to stop? How does it determine which amino acid to add? termination factor How does it know when to tell the ribosome to quit? How does it avoid telling the ribosome prematurely? Special events of the ribosome & assembly The initial assembly event requires teamwork. Both prokaryotes and eukaryotes require an mRNA, the small ribosomal subunit, and a tRNA that can read the codon AUG, so we will focus on these commonalities. SO!! The ribosomal subunits are allowed to lurk nearby (or better, draw the small subunit on your benchtop and keep the mRNA nearby for ‘scanning’) When small ribosomal subunit + tRNA that matches AUG + mRNA team forms, the LARGE ribosomal subunit is now allowed to join, and the game is ON! As tRNA visitors diffuse by, if any can match the AUG codon near the 5' end of the mRNA, finish drawing the ribosome such that the P site is vacant and the A site (see lab manual p. 4-3) is ready for the next tRNA
