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GeneArt Engineered Cell Models The new workhorse cell lines for genome editing The world’s largest collection of engineered cell lines, great for rapid hypothesis testing Get instant access to over 14,000 validated, ready-to-go cell lines engineered using CRISPR-Cas9 technology. The Invitrogen™ GeneArt™ Engineered Cell Models collection offers a superior resource for functional characterization of genes and their role in cellular processes, signaling pathways, disease development and progression, and drug response. These cell lines are validated with defined genome modifications, on the shelf and ready to ship. Traditional cellbased assays compare patient cells with cells from healthy individuals, which can differ genetically in thousands of ways. GeneArt Engineered Cell Models offer a system where the control and mutant cell lines differ only by the mutation you wish to test, enabling greater confidence in your results. Now you can transition from genomic information to functional characterization faster than ever before— accelerate your research with the new workhorse cell lines for genome editing. GeneArt Engineered Cell Models are: •Affordable—academic pricing is comparable to patientderived cell lines •Verified—extensive quality control •Comprehensive—the single largest bank of isogenic cell lines with over 14,000 available •Proven—used in hundreds of research labs and published in top scientific journals •Available—catalog cell lines can be delivered within one week Haploid cellular models represent an attractive alternative for in vitro genetic analyses of molecular, cellular, and developmental events. Haploid cellular models are easier to generate than modified diploid cell lines, as only one allele needs to be targeted to obtain a complete loss-of-function phenotype. The HAP1 cell line is a near-haploid human cell line that allows rapid gene editing to be performed. HAP1 cells are adherent with fibroblast-like morphology (Figure 1). These cells are ideal for applications where a workhorse cell line is needed for rapid hypothesis testing, similar to the way HEK 293 and HeLa cells have been used to accelerate research programs for years. The GeneArt Engineered Cell Models collection includes knockout cell lines generated using HAP1 cells and a wide range of other cell lines. Diploid Haploid Mutation masked by second copy Mutation leads to knockout •Controlled—each cell line comes with a matched parental cell line HAP1 is the workhorse cell line for genome editing experiments Knockout cell lines are the most powerful in vitro models available for understanding gene function. One of the challenges to the rapid generation of knockout cell lines in mammalian cell models is the diploid nature of the genome, which requires mutations that eliminate gene function to be introduced into both copies of the gene to ensure complete knockout. Figure 1 Figure 1. Spectral karyotyping and brightfield imaging of HAP1 cells. HAP1 cells have defined copy number and unambiguous genotyping. Confidence in your results GeneArt Engineered Cell Models consist of a clonal cell line bearing your mutation of choice, which has been verified by Sanger sequencing. All GeneArt Engineered Cell Models ship with the unedited, isogenic parental cell line, providing the perfectly matched control to enable confidence in your results. Wild type cell line Single-cell dilution Cas9 CRISPR-Cas9 genome editing Pre-engineering cell line validation • SNP Array 6.0 • Sanger sequencing • Digital PCR • RT-PCR Post-engineering cell line validation Wild type cell line Clonal mutant cell line Isogenic pair of cell lines Clonal mutant cell line Figure 2. GeneArt Engineered Cell Models undergo extensive characterization before and after gene editing. When appropriate, the originating wild type cell line can be diluted to a single cell ensure a clonal population exists prior to gene editing. This clone goes through cell line validation The isogenically matched cell pairs that Figureto 3 and if successful is taken forward to gene editing. The resulting clonal mutant cell line goes through you receive eliminate the background the same rigorous cell line validation process. Figure 4 genetic variation observed with other cell models and provide you with greater confidence that any differences observed between the mutant and the parental cell line are due to your specific mutation of interest. All of our cell lines undergo extensive QC and validation (Figure 2). Genome modulation and engineering services Looking for something more custom? We can help you from start to finish. For more information, contact us at [email protected] Accelerate your research and search for your cell line at thermofisher.com/engineeredcells For Research Use Only. Not for use in diagnostic procedures. © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO210908 1115